Kinetic characterisation and thermal inactivation study of partially purified red pepper (Capsicum annuum L.) peroxidase

Peroxidase (POD) from red sweet pepper cultured under an integrated system was partially purified, using a combination of phase partitioning with Triton X-114 and ammonium sulphate fractionation between 30 and 80%. The enzyme presented a single band in PAGE only when 4-MN was used as substrate. Optimum activity using ABTS as the H-donor was obtained at pH 4.5 and the apparent kinetic parameters V_m and K_M were calculated for both ABTS and H₂O₂, showing a K_M value in the same order in both cases (0.495 and 1.32 mM, respectively). The effect of several reducing agents was studied, ascorbic acid being the most active. The study of thermal inactivation showed a first-order inactivation kinetic, and the Arrhenius plot yielded a straight line with a slope equivalent to an activation energy of 151 kJ/mol. Significant inactivation occurred at temperature >40 °C and the D value for 5 min was 44.5 °C.     

Energy-saving light positioning using heuristic search

A new definition is given to the problem of light positioning in a closed environment, aiming at obtaining, for a global illumination radiosity solution, the position and emission power for a given number of lights that provide a desired illumination at a minimum total emission power. Such a desired illumination is expressed using minimum and/or maximum values of irradiance allowed, resulting in a combinatory optimization problem. A pre-process computes and stores irradiances for a pre-established set of light positions by means of a radiosity random walk. The reuse of photon paths makes this pre-process reasonably cheap. Different heuristic search algorithms, combined to linear programming, are discussed and compared, from the simplest hill climbing strategies to the more sophisticated population-based and hybrid approaches. The paper shows how the presented approaches make it possible to obtain a good solution to the problem at a reasonable cost.     

Assessing experimental visceral pain in dairy cattle: A pilot,prospective, blinded,randomized, and controlled study focusing on spinal pain proteomics

Few studies have verified the validity of behavioral and physiological methods of pain assessment in cattle. This prospective, blinded, randomized controlled experimental study aimed to validate different methods of pain assessment during acute and chronic (up to 21 d postintervention) conditions in dairy cattle, in response to 3 analgesic treatments for traumatic reticuloperitonitis. Cerebrospinal fluid (CSF) biomarkers and mechanical sensitization were measured as indicators of centralized pain. Proteomics in the CSF were examined to detect specific (to pain intensity) and sensitive (responsive to analgesia) markers. Recordings of spontaneous behavior with video analysis, telemetered motor activity, pain scales, electrodermal activity, and plasma cortisol concentration were quantified at regular intervals. Cows were assigned to group 1 (n = 4, standard control receiving aspirin), group 2 (n = 5, test group receiving preemptive tolfenamic acid), or group 3 (n = 3, positive control receiving preemptive multimodal analgesia composed of epidural morphine, plus tolfenamic acid and butorphanol). Rescue analgesia was administered as needed. Generalized estimating equations tested group differences and the influence of rescue analgesia on the measurements. All 3 groups demonstrated a long-term decrease in a CSF protein identified as transthyretin. The decrease in transthyretin expression inversely correlated with the expected level of analgesia (group 1 < 2 < 3). Moreover, in group 1, CSF noradrenaline decreased long term, cows were hypersensitive to mechanical stimulation, and they demonstrated signs of discomfort with higher motor activity and “agitation while lying” recorded from video analysis. Decreased “feeding behavior,” observer-reported pain scales, electrodermal activity, and plasma cortisol concentration were inconsistent to differentiate pain intensity between groups. In summary, changes in CSF biomarkers and mechanical sensitization reflected modulation of central pain in dairy cows. The spontaneous behavior “agitation while lying” was the only behavioral outcome validated for assessing acute and chronic pain in this visceral pain model.     

Study of different nectarine cultivars and their suitability for fresh‐cut processing

Nectarine [Prunus persica (L.) Batsch] is a sought‐after fruit due to its properties. In addition, minimally processed produces are more and more accepted by consumers. Four nectarine cultivars (‘Early Top’, ‘Nectaprima’, ‘Big Top’ and ‘Venus’) were selected to study their suitability for minimal processing. Samples were processed in a clean room at 8 °C, washed them in water (4 °C) and stored at 4 °C in modified atmosphere packaging. The samples were analysed at 0, 3, 6 and 9 days of storage, and quality parameters as well as functional compounds (polyphenols and polyphenol oxidase activity) were studied. Principal component analysis (PCA) was carried out to assess the relationship between nectarine cultivars and determined parameters. In conclusion, ‘Early Top’ and ‘Nectaprima’ cultivars showed the best quality results and therefore a promising suitability for fresh‐cut processing. Nevertheless, the remaining cultivars should not be ruled out due to their functional content, advising additional conservation treatments for them.     

Effects of α-, β- and maltosyl-β-cyclodextrins use on the glucoraphanin–sulforaphane system of broccoli juice

Cyclodextrins (CDs) are macromolecules with several industrial applications, being particularly used in the food industry as health-promoting compounds protection agents, as flavour stabilizers, or to eliminate undesired tastes and browning reactions, among others. This study shows the effects of α- (10, 30 and 40 mmol L⁻¹), β- (3, 6 and 10 mmol L⁻¹) and maltosyl-β-CDs (30, 60 and 90 mmol L⁻¹) use on the health-promoting glucoraphanin–sulforaphane system of a broccoli juice up to 24 h at 22 °C. Maltosyl-β-CD (90 mmol L⁻¹) highly retained glucoraphanin content after 24 h at 22 °C, showing better effectiveness than β-CD (10 mmol L⁻¹). Sulforaphane was efficiently encapsulated with β-CD at just 3 mmol L⁻¹, and the sulforaphane formed was stable during 3 h at 22 °C. On the other hand, 40 mmol L⁻¹ α-CD retained a high glucoraphanin content in broccoli juice. In contrast, glucoraphanin levels in juice without CDs decreased by 71% after 24 h. Consequently, CDs addition may potentially preserve glucoraphanin in this broccoli juice during industrial processing with the possibility to be later transformed by endogenous myrosinase after ingestion to the health-promoting sulforaphane. © 2018 Society of Chemical Industry     

Animal welfare and meat quality: The perspective of Uruguay,a “small” exporter country

Public sensitivity towards animal welfare has risen in recent years. Uruguay is a primary meat exporter. Therefore, it is compulsory not only to provide good quality and safe meat, but also to project a welfare friendly image. Uruguayan meat production systems are mainly based on rangeland pastures but, due to international meat prices and the opening of new markets, intensive fattening systems increased. These systems include a wide range of feeding alternatives between pasture and concentrate utilization, involving differences in terms of animal welfare, carcass and meat quality, that require to be studied. Accordingly, some husbandry practices associated mainly with extensive systems must be evaluated, as well as their applicability to international recommendations related to pre-slaughter handling which may not be suitable for local conditions. In the present paper we share scientific results related to the impact of different production systems, husbandry practices and pre-slaughter procedures associated to animal welfare and meat quality in Uruguayan conditions.     

Production of a potential collagenolytic protease by nejayote fermentation with Aspergillus oryzae

Nejayote is the wastewater obtained from maize lime cooking. It was used for Aspergillus oryzae growth and enzyme production. Protein production and collagenolytic activity were evaluated to test the effects of pH, spore inoculum and nejayote content in a 3³ factorial design. No collagenolytic activity was observed in the supernatant of the fermentation without nejayote. Using nejayote at 5% increased 1.5 times the fungus growth in comparison with nejayote 1% and 3%; and the maximum collagenolytic activity was reached after 3 days at pH 8 (P < 0.05). After sequencing, a 32 kDa alkaline protease was identified. Along with the alkaline conditions produced by the inclusion of nejayote solids, this waste is an important source of carbohydrates that improved the growth of A. oryzae to produce a protease that has the potential to break collagen. This permits a prospective revalorisation of a waste material to get a product with multiple biotechnological uses.     

The impact of wind resource spatial variability on floating offshore wind farms finance

Wind resource availability determines the financial performance of wind farms as it is directly related to production. Offshore wind developers require great investments to design, build, operate and dismantle offshore wind farms. Furthermore, the investments in the offshore floating wind sector are expected to increase in the future. Because of that, the assessment of the variability of the investments, mainly because of the wind resource variability, seems to be a crucial step in the design methodology. Consequently, a flexible methodology for supporting offshore floating wind farm optimal location assessment is presented in this paper. The proposed methodology is focused on including the offshore wind resource variability and its influence on the power production of floating wind farms, as well as on the main financial indicators (internal rate of return, net present value, pay‐back period and cost of energy). The methodology is applied to the north coast of Spain, and it allows to identify the most promising sites for offshore wind farms deployment. Differences on the cost of energy up to 100% can be found in the area under study. The methodology proposed has been conceived to be site‐independent and applied at any spatial and time horizon. Copyright © 2017 John Wiley & Sons, Ltd.