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排序方式: 共有402条查询结果,搜索用时 265 毫秒
91.
Heterologous expression of specific K+ channels in T lymphocytes: functional consequences for volume regulation 总被引:1,自引:0,他引:1
It has been postulated that the K+ channel isoform Kv1.3 plays a role in regulatory volume decrease (RVD) in response to hypotonic shock. We show that a mouse cytotoxic T-lymphocyte line, CTLL-2, is devoid of voltage-dependent K+ channels and is unable to volume regulate. Transient transfection of these cells with Kv1.3 reconstitutes their ability to volume regulate. As predicted by our model, this ability depends critically on volume-induced changes in membrane potential and the isoform of the K+ channel used. When the cells were transfected with Kv3.1, an isoform believed to be expressed in a specific subclass of mouse thymocytes, the CTLL-2 cells did not show RVD. The difference in the ability of the two isoforms to confer the capacity for RVD is expected from differences in the voltage dependence of activation of the channels, according to our proposed model for RVD. The experimental approach that we use, transient transfection and panning to select positive transfectants, is highly effective; it has a > 95% efficiency. This method, and this cell line, may be important tools in studying lymphocyte K+ channels and their function in situ. 相似文献
92.
Metastatic carcinoma to the testis is very rare. Metastasis of prostate adenocarcinoma to testis was detected incidentally after bilateral orchiectomy for hormonal management of metastatic prostate carcinoma. The metastatic lesion was not identified in physical examination or in macroscopic dissection of the testis after surgery. Microscopy revealed an adenocarcinoma which, given the history of the patient and a positive immunohistochemical stain for PSA, was identified as metastatic prostatic adenocarcinoma. 相似文献
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E Deutsch 《Canadian Metallurgical Quarterly》1997,91(7):698-700; discussion 701
Clinical research can be therapeutic or purely scientific. The therapeutic trial is usually chosen to obtain treatment liberty. On the other hand, the physicians in controlled clinical trials have relatively little choice of treatments. In Germany, special statutes and the general law limit clinical trials. The sec. 41 AMG, 18 MPG allow therapeutical trials under special circumstances. There are requirements for clinically controlled trials, especially the provision to seek the approval of an ethic commission. 相似文献
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TN Alim RB Rosse FJ Vocci T Lindquist SI Deutsch 《Canadian Metallurgical Quarterly》1995,18(2):183-195
Fifty cocaine-dependent patients completed a 2-week double-blind, double-dummy, parallel-group comparison of four dosage levels of diethylpropion and placebo. This clinical trial was designed to evaluate both diethylpropion's ability to attenuate cocaine cue-induced craving and its potential for development as a medication with cocaine-agonist properties. The results indicated that diethylpropion was not superior to placebo and confirmed earlier reports that craving for cocaine diminishes over the course of an inpatient hospitalization. Moreover, the results showed that the cocaine cue-induced craving paradigm employed is effective in stimulating craving for cocaine. Medications that are effective in attenuating this type of "conditioned" craving may have relevance to the breaking of the cycle of relapse and long-term treatment of cocaine dependence. Diethylpropion may not be an appropriate candidate for future medication development because of its lack of obvious therapeutic efficacy and the emergence of a significant number of side effects. However, a cocaine-agonist medication strategy may be appropriate for a subgroup of cocaine-dependent patients with coexisting attention deficit-hyperactivity disorder. 相似文献
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RS Meyer S Deutsch JC Maymir DB Geselowitz JM Tarbell 《Canadian Metallurgical Quarterly》1997,25(6):1081-1091
Three-dimensional laser Doppler velocimetry measurements were acquired in a mock-circulatory loop proximal to a Bj?rk-Shiley monostrut valve in the mitral position, and synchronous ensemble-averaging was applied to form an "average" beat. Two axial locations in the regurgitant flow region of the valve (in the minor orifice) were mapped, and maximum Reynolds shear stresses were calculated. A large spike in regurgitant flow was noted at the beginning of systole, which may be the squeeze flow phenomenon computed by other researchers. A region of sustained regurgitant flow 50 msec later was the focus of this study. Maximum velocities of approximately 3.7 mps were noted, and maximum Reynolds shear stresses of approximately 10,000 dyne/cm2 were calculated. Comparisons were made of two-dimensional (ignoring tangential component) versus three-dimensional shear stresses, and, in this case, in regions of high stress, the differences were insignificant. This suggests that the tangential component of velocity can probably be ignored in similar measurements where the tangential velocity is likely to be small. 相似文献
99.
Shao HM Truong TK Deutsch LJ Yuen JH Reed IS 《IEEE transactions on computers. Institute of Electrical and Electronics Engineers》1985,(5):393-403
A pipeline structure of a transform decoder similar to a systolic array is developed to decode Reed-Solomon (RS) codes. An important ingredient of this design is a modified Euclidean algorithm for computing the error-locator polynomial. The computation of inverse field elements is completely avoided in this modification of Euclid's algorithm. The new coder is regular and simple, and naturally suitable for VLSI implementation. An example illustrating both the pipeline and systolic array aspects of this decoder structure is given for a RS code. 相似文献
100.