Extension of distance measurement range in a sinusoidal wavelength-scanning interferometer using a liquid-crystal wavelength filter with double feedback control

The optical path difference (OPD) and amplitude of a sinusoidal wavelength scanning (SWS) are controlled with a double feedback control system in an interferometer, so that a ruler marking every wavelength and a ruler with scales smaller than a wavelength are generated. These two rulers enable us to measure an OPD longer than a wavelength. A liquid-crystal Fabry-Perot interferometer (LC-FPI) is adopted as a wavelength-scanning device, and double sinusoidal phase modulation is incorporated in the SWS interferometer. Because of a high resolution of the LC-FPI, the upper limit of the measurement range can be extended to 280 microm by the use of the phase lock where the amplitude of the SWS is doubled in the feedback control. The ruler marking every wavelength is generated between 80 microm and 280 microm, and distances are measured with a high accuracy of the order of a nanometer in real time.     

Study of AC Loss Characteristics of HTS-coated Conductor with?Magnetic Substrate Using FEM Analysis

A high-T _c superconducting (HTS)-coated conductor with a magnetic substrate is promising as a low-cost conductor because Ni alloy can be well texturized by a simple process. However, it has been reported that the magnetism of the substrate makes an AC transport current loss and an AC magnetization loss increase from those losses of the HTS-coated conductor of a nonmagnetic substrate sharply. However, the assembled conductor which bundled many HTS-coated conductors with a magnetic substrate is hardly reported. In this paper, the AC loss and the current distribution in the superconducting layer of the HTS-coated conductor with a magnetic substrate are analyzed using the edge-based hexahedral finite element method (FEM) taking account of both the nonlinear E?CJ characteristics and the nonlinear magnetic property of the substrate at 77 K. Moreover, AC loss characteristics of HTS-coated conductors with a magnetic substrate arranged with an infinite number of the coated conductors are examined and the effect of the gap length between adjacent conductors on the AC loss is also investigated.     

X-ray microdiffraction investigation of crystallinity and strain relaxation in Ge thin lines selectively grown on Si(0 0 1) substrates

We used X-ray microdiffraction (XRMD) to investigate the crystallinity and strain relaxation of Ge thin lines with widths of 100, 200, 500 and 1000 nm selectively grown on Si(0 0 1) substrates using a patterned SiO₂ mask by chemical vapor deposition. The variations of the strain relaxation in the line and width directions were also investigated in Ge thin lines with a width of 100 nm. After growth, crystal domains with very small tilt angles were detected in Ge lines with all four line widths. The tilt angle range was larger in thinner Ge lines. After annealing at 700 °C, the formation of a single, large domain with a specific tilt angle was detected by XRMD for Ge thin lines with widths of 100 and 200 nm. These experimental results reflect the effects of SiO₂ side walls around the Ge thin lines on crystallinity and strain relaxation of Ge.     

Growth arrest and chromosome instability in aneuploid yeast

Aneuploid generation and stability are biologically important. In the present study, we investigated fission yeast aneuploids, focusing on the process through which aneuploidy is resolved into stable euploidy. The viability and growth patterns of aneuploid spores were greatly influenced by culture conditions, including nutrition and temperature. Germ tube formation and DNA synthesis in a major portion of aneuploids were greatly delayed or arrested. Observation of individual spores and their growth profiles revealed that a certain type(s) of aneuploid resolved its aneuploidy into normal euploids through anomalous cell divisions, which in many cases produced dead cells. Another type of aneuploid, disomy of chromosome 3, the only maintainable aneuploid between n and 2n, showed a peculiar cell division arrest phenotype under a certain growth condition. Microcolonies that formed from this type of aneuploid often contained a population of cells that became incompetent for cell division. This cell division arrest was not due to a nutritional limitation. During this peculiar process of colony formation, stable haploids or diploids were frequently produced. All other types of aneuploids are usually inviable, at least under our experimental conditions. To examine the aneuploid issue more systematically, we constructed a system to select for disomy of chromosome 1 or 2 using intragenic complementation of ade6-M210 and -M216 alleles. This genetic selection system revealed that fission yeast aneuploids can be stabilized through structural chromosome changes, including partial duplication and circular mini-chromosomes.     

Tetraphenylporphyrin Enters the Ring: First Example of a Complex between Highly Bulky Porphyrins and a Protein**

Tetraphenylporphyrin (TPP) is a symmetrically substituted synthetic porphyrin whose properties can be readily modified, providing it with significant advantages over naturally occurring porphyrins. Herein, we report the first example of a stable complex between a native biomolecule, the haemoprotein HasA, and TPP as well as its derivatives. The X-ray crystal structures of nine different HasA-TPP complexes were solved at high resolutions. HasA capturing TPP derivatives was also demonstrated to inhibit growth of the opportunistic pathogen Pseudomonas aeruginosa. Mutant variants of HasA binding FeTPP were shown to possess a different mode of coordination, permitting the cyclopropanation of styrene.