Ornithine-delta-aminotransferase expression and ornithine metabolism in cultured epidermal keratinocytes: toward metabolic sink therapy for gyrate atrophy

There is now strong evidence that the chorioretinal degeneration associated with ornithine-delta-aminotransferase (OAT) deficiency is a consequence of hyperornithinemia. Therefore development of a metabolic system for clearing ornithine from the circulation is being pursued as a potential treatment. The skin is considered an attractive location for such a metabolic system because autologous cells can be safely and easily utilized. This study was undertaken to determine the ornithine metabolizing capacity of epidermal keratinocytes expressing normal and superphysiologic amounts of OAT. The data show that overexpression of OAT in keratinocytes cultured from a gyrate atrophy patient restores ornithine metabolism and results in a rate of ornithine disappearance from the medium that is significantly higher than the rate of disappearance from the medium bathing normal keratinocytes. In addition, OAT activity determined in soluble protein prepared from sonicates suggests that the capacity to maintain plasma ornithine within the normal range is contained within an accomplishable graft of keratinocytes overexpressing OAT. However, the actual rate of ornithine disappearance from the media was significantly less than predicted from enzyme activity assays. Following ornithine metabolite production by intact cells suggests that ornithine metabolism is limited primarily by clearance of downstream metabolites, as opposed to substrate delivery.     

Numerical modeling of near corona wire electrohydrodynamic flow in a wire-plate electrostatic precipitator

Numerical modelling of the flow velocity fields for the near corona wire electrohydrodynamic (EHD) flow was conducted. Solutions of the steady, two-dimensional momentum equations have been computed for a wire-plate type electrostatic precipitator (ESP). The equations were solved in the conservative finite-difference form on a fine uniform rectilinear grid of sufficient resolution to accurately capture the momentum boundary layers. The numerical procedure for the differential equations was used by SIMPLEST algorithm. The CFD code coupled with Poisson's electric field, ion transport equations and the momentum equation with electric body force, was used for the numerical simulation with the Chen-Kim k-epsiv turbulent model. The numerical results show that EHD secondary flow was clearly visible in the downstream regions of the corona wire despite the low Reynolds number for the electrode (Re _cw=12.4). Secondary flow vortices caused by the EHD increases with increasing discharge current or EHD number, hence pressure drop of ESP increases     

Rapid relief of anxiety in cancer patients with both alprazolam and placebo

This study evaluated 36 cancer patients who were enrolled in a randomized, double-blind, placebo-controlled trial conducted over a 4-week period to evaluate the efficacy of alprazolam in the treatment of anxiety associated with cancer. Hamilton Anxiety Scale scores declined significantly between baseline and the end of the first week of the study in both treatment groups. There was no significant difference in response between the patients receiving alprazolam and placebo. Similar results were obtained from other instruments. These results suggest that nondrug factors or spontaneous improvement may play a more important role than pharmacotherapy in the treatment of anxiety associated with cancer.     

Physical model of drain conductance, gd, degradation ofNMOSFET's due to interface state generation by hot carrierinjection

Degradation of analog device parameters such as drain conductance, g_d, due to hot carrier injection has been modeled for NMOSFET's. In this modeling, mobility reduction caused by interface state generation by hot carrier injection and the gradual channel approximation were employed. It has been found that g_d degradation can be calculated from linear region transconductance, g_m, degradation which is usually monitored for hot carrier degradation of MOSFET's. The values of g_d degradation calculated from g_m degradation fit well to the measured values of g_d degradation The dependence of the g_d degradation lifetime on L_eff has been also studied, this model also provides an explanation of the dependence on L_eff. The model is then useful for lifetime predictions of analog circuits in which g_d degradation is usually more important than g_m degradation     

Spectrally stable Er-fiber laser for application in phase-sensitive optical time-domain reflectometry

A high degree of frequency stability in an Er-fiber laser with the Fabry-Perot configuration was achieved using a long (25-km) optical-feedback loop. The frequency-drift rate was determined to be <1 MHz/min by observing temporal fringes in an unbalanced Mach-Zehnder interferometer. Instantaneous linewidth measured with a delayed self-heterodyne interferometer was less than the 3-kHz resolution limit. The laser was utilized in an optical time-domain reflectometry experiment to detect and locate a time-varying phase perturbation applied to an optical fiber.     

Deactivation of copper-ion-exchanged hydrogen-mordenite-type zeolite catalyst by SO2 for no reduction by NH3

Deactivation of copper-ion-exchanged hydrogen-mordenite-type zeolite catalyst by SO₂ for NO reduction by NH₃ was examined in a fixed-bed flow reactor. The deactivation of the catalyst was strongly dependent on reaction temperature. At high reaction temperatures over 300°C, the catalyst did not lose its initial activity up to 50 h of operation, regardless of SO₂ feed concentration from 500 to 20,000 ppm. However, at low reaction temperatures near 250°C, apparent deactivation did occur. Changes in the physicochemical properties such as surface area and sulfur content of deactivated catalyst well correlated with catalyst activity, depending upon reaction temperatures. The deactivation was due to pore blocking and/or filling by deactivating agents, which plugged and/or filled the pores of catalyst. The deactivating agents deposited on the catalyst surface were presumed to be (NH₄)₂SO₄ and/or (NH₄)HSO₄ from the results of TGA and ion-chromatography measurement.     

A novel high-efficiency linear transmitter using injection-locked pulsed oscillator

This letter presents a novel high-efficiency linear transmitter using pulse-width modulation (PWM). An envelope of radio frequency (RF) input signal is modulated by the PWM. The modulated signal is applied to the gate bias of a class F injection-locked power oscillator and switches it on and off. By filtering the pulsed oscillating output signal of the injection-locked oscillator using high-Q bandpass filter, the input signal is restored. This technique enables the transmitter to have high efficiency with good linearity. Also, there is little distortion near saturation point of an active device. The measured results show efficiency of 54.6% and very good linearity in PCS band at 26.4-dBm output power.     

R.A.P.I.D. Reconstructive alternative for prosthetics in implant dentistry

As stated at the outset, this technique is not for every case. Each case must be selected with great care, consideration and careful planning. In concert with observing the Implant Lighthouses, this technique can produce a successful case. It can be done to save time, without compromising the prescribed techniques and quality.