Cardenolides from the methanolic extract of Nerium oleander leaves possessing central nervous system depressant activity in mice

OBJECTIVE: To identify characteristics associated with provision of bystander CPR in witnessed out-of-hospital cardiac arrest cases. METHODS: An observational, prospective, cohort study was performed using cardiac arrest cases as identified by emergency medical services (EMS) agencies in Oakland County. MI, from July 1, 1989, to December 31, 1993. All patients who sustained a witnessed arrest prior to arrival of EMS personnel were reviewed. RESULTS: Of the 927 patients meeting entry criteria, the 229 patients receiving bystander CPR were younger: 60.9 +/- 14.7 vs 67.9 +/- 14.7 years (p < 0.01). Most (76.6%) cardiac arrests occurred in the home. In a multivariate logistic model, only the location of arrest outside the home was a significant predictor of receiving bystander CPR [odds ratio (OR) 3.8; 99% CI 2.5, 5.9]. Arrests outside the home were associated with significantly improved outcome, with 18.2% of out-of-home and 8.2% of in-home victims discharged from the hospital alive (OR 2.5; 99% CI 1.4, 4.4). CONCLUSION: Patients who have had witnessed cardiac arrests outside the home are nearly 4 times more likely to receive bystander CPR, and are twice as likely to survive. This observation emphasizes the need for CPR training of family members in the authors' locale. This phenomenon may also represent a significant confounder in studies of out-of-hospital cardiac arrest and resuscitation.     

Effects of growth factors and basement membrane proteins on the phenotype of U-373 MG glioblastoma cells as determined by the expression of intermediate filament proteins

Various growth factors and basement membrane proteins have been implicated in the pathobiology of astrocytomas. The goal of this study was to determine the relative contribution of these two factors in modulating the phenotype of U-373 MG glioblastoma cells as determined by the expression of the intermediate filament proteins glial fibrillary acidic protein, vimentin, and nestin. For these determinations, cells plated in serum-free medium were treated either with growth factors binding to tyrosine kinase receptors including transforming growth factor-alpha, epidermal growth factor, platelet-derived growth factor-AA, basic fibroblast growth factor, and insulin-like growth factor-1 or with basement membrane proteins including collagen IV, laminin, and fibronectin. The changes in the expression levels of intermediate filament proteins in response to these treatments were analyzed by quantitation of immunoblots. The results demonstrate that collagen IV and growth factors binding to tyrosine kinase receptors decrease the glial fibrillary acidic protein content of U-373 MG cells. Growth factors binding to tyrosine kinase receptors also decrease the vimentin content of these cells but do not affect their nestin content. On the other hand, basement membrane proteins decrease the nestin content of U-373 MG cells but do not affect their vimentin content. The significance of these results with respect to the role played by different factors in modulating the phenotype of neoplastic astrocytes during tumor progression is discussed.     

Effect of separate pod and root zone temperatures on yield and seed composition of three Spanish cultivars of groundnut (Arachis hypogaea L)

The effect of separate pod and root temperature regimes (all four combinations of 28/22 and 40/34 °C day/night temperature), imposed from the time of peg penetration until harvest, on yield and seed composition of three Spanish genotypes of groundnut (Arachis hypogaea L) was investigated. A decrease in pod temperature from 40/34 to 28/22 °C increased yield and oil, starch and protein mass per plant irrespective of root temperature. Additionally, a reduction in pod temperature decreased protein concentration and increased the sum of oil and starch concentration at a root temperature of 28/22 °C, whereas at a root temperature of 40/34 °C a decrease in pod temperature increased protein concentration. Root temperature reduction diminished oil concentration of genotypes AH 6179 and TMV 2 at a pod temperature of 40/34 °C. A decline in pod temperature affected fatty acid composition through a decrease in palmitic acid irrespective of root temperature and an increase in linoleic acid at a root temperature of 28/22 °C. A root temperature effect on fatty acid composition was not detected. It is concluded that field management practices and choice of genotype can influence groundnut yield and seed composition through effects on pod and root temperature. © 2001 Society of Chemical Industry     

Gelatin controversies in food,pharmaceuticals, and personal care products: Authentication methods,current status,and future challenges

Gelatin is a highly purified animal protein of pig, cow, and fish origins and is extensively used in food, pharmaceuticals, and personal care products. However, the acceptability of gelatin products greatly depends on the animal sources of the gelatin. Porcine and bovine gelatins have attractive features but limited acceptance because of religious prohibitions and potential zoonotic threats, whereas fish gelatin is welcomed in all religions and cultures. Thus, source authentication is a must for gelatin products but it is greatly challenging due to the breakdown of both protein and DNA biomarkers in processed gelatins. Therefore, several methods have been proposed for gelatin identification, but a comprehensive and systematic document that includes all of the techniques does not exist. This up-to-date review addresses this research gap and presents, in an accessible format, the major gelatin source authentication techniques, which are primarily nucleic acid and protein based. Instead of presenting these methods in paragraph form which needs much attention in reading, the major methods are schematically depicted, and their comparative features are tabulated. Future technologies are forecasted, and challenges are outlined. Overall, this review paper has the merit to serve as a reference guide for the production and application of gelatin in academia and industry and will act as a platform for the development of improved methods for gelatin authentication.     

In vitro synergism of antimutagenic and antioxidant activities of Phoenix dactylifera fruit

The availability of total phenolics of date flesh (Pheonix dactylifera) in aqueous and acidified methanol and in vitro synergism of their antimutagenic and antioxidant activities were investigated. The antimutagenic activities of date flesh extracts tested against Salmonella strains TA-98 and TA-100 using Ames bacterial testing were in the range of 36.47 to 79.74% against the standard mutagens potassium dichromate and sodium azide. The antioxidant activities assessed as maximal inhibitory concentrations (IC₅₀) for DPPH radicals and linoleic acid peroxidation inhibition were 812.90 to 2,076.10 μg/mL and 54.43 to 80.89%, respectively. The total phenolic contents of date flesh extracts (35.76 to 114.09mg/g of gallic acid equivalents) were found to be correlated with the biological activities. Use of 0.5 N acidified methanol was efficient for extraction of phenolic compounds with retained antioxidant and antimutagen activities. Dates are a good candidate as a source for development of chemotherapeutic drugs, nutracueticals, and functional food ingredients.     

Numerical techniques for solving solidification and melting phase change problems

This article reviews several mathematical formulations including the corresponding boundary conditions for numerical predictions of solidification and melting. Emphasis is on techniques that are used in solving solid–liquid interface phenomena. The fixed grid enthalpy method is reviewed based on the solution techniques of conduction and convection related phase change problems. Variable grid methods are categorized and then analyzed based on their accuracy, computational efforts and convergence characteristics. The article concludes with some guidance for selecting the accurate solution techniques for solving solidification and melting problems.     

Thermal performance of a novel rooftop solar micro-concentrating collector

Concentrating solar thermal systems offer a promising method for large scale solar energy collection. Although concentrating collectors are generally thought of as large-scale stand-alone systems, there is a huge opportunity to use novel concentrating solar thermal systems for rooftop applications such as domestic hot water, industrial process heat and solar air conditioning for commercial, industrial and institutional buildings. This paper describes the thermal performance of a new low-cost solar thermal micro-concentrating collector (MCT), which uses linear Fresnel reflectors, and is designed to operate at temperatures up to 220 °C. The modules of this collector system are approximately 3 m long by 1 m wide and 0.3 m high. The objective of the study is to optimise the design to maximise the overall thermal efficiency. The absorber is contained in a sealed enclosure to minimise convective losses. The main heat losses are due to natural convection inside the enclosure and radiation heat transfer from the absorber tube. In this paper we present the results of a computational and experimental investigation of radiation and convection heat transfer in order to understand the heat loss mechanisms. A computational model for the prototype collector has been developed using ANSYS–CFX, a commercial computational fluid dynamics software package. The numerical results are compared to experimental measurements of the heat loss from the absorber, and flow visualisation within the cavity. This paper also presents new correlations for the Nusselt number as a function of Rayleigh number.     

Novel multiplex PCR-RFLP assay discriminates bovine,porcine and fish gelatin substitution in Asian pharmaceuticals capsule shells

Gelatin is widely used in pharmaceuticals as a protective coating, such as soft and hard capsule shells. However, the animal source of gelatin is a sensitive issue because certain gelatins such as porcine and bovine gelatins are not welcome in Halal, Kosher and Hindus’ consumer goods. Recently, we have documented DNA barcoding and multiplex PCR platforms for discriminating porcine, bovine and fish gelatins in various fish and confectionary products; but those assays were not self-authenticating and also not tested in highly refined pharmaceutical products. To address this knowledge gap, here we report a self-authenticating multiplex PCR-restriction fragment length polymorphism (RFLP) assay to identify animal sources of various gelatin in pharmaceutical capsules. Three different restriction enzymes, BsaAI, Hpy188I and BcoDI were used to yield distinctive RFLP patterns for gelatin-based bovine (26, 94 bp), fish (97, 198 bp) and porcine (17, 70 bp) DNA in control experiments. The specificity was cross-tested against 16 non-target species and the optimised assay was used to screen gelatin sources in 30 halal-branded pharmaceuticals capsule shells. Bovine and porcine DNA was found in 27 and 3 of the 30 different capsules products. The assay was suitable for detecting 0.1 to 0.01 ng total DNA extracted from pure and mixed gelatins. The study might be useful to authenticate and monitor halal, kosher, vegetarian and Hindu compliant pharmaceuticals, foods and cosmetics.     

Optimization and surface modification of silk fabric using DBD air plasma for improving wicking properties

It is necessary to study the suitable effect of plasma parameters, such as exposure time and applied voltage on the wicking properties of silk fabrics. Wicking property of silk fabric has been improved by using air dielectric barrier discharge plasma treatment. The plasma treatment has been utilized to modify the surface properties of silk fabrics. Untreated and plasma-treated fabrics have been characterized by contact angle Goniometer, wicking test, Wet-out time, scanning electron microscope, Attenuated Total Reflection Fourier Transforms Infrared spectrometer (ATR-FTIR) and dye uptake test. The ATR-FTIR characterization shows that the hydroxyl functional group has been increased after plasma treatment of silk fabric. The experimental results of wicking rate and Wet-out time of the plasma-treated silk fabrics have shown significant improvement in hydrophilic properties that could be confirmed by contact angle measurement which is close to 0°. The tensile strength of untreated and plasma-treated fabrics has been measured to confirm the enhanced surface property.