A study on the antimicrobial activity of thymol intended as a natural preservative

A quantitative investigation on the inhibitory activity of thymol against some microorganisms that could represent a potential spoilage risk both in acid and mild thermally treated foods is presented in this work. In order to assess potential biostatic or biocidal activity of thymol, both the growth kinetics and dose-response profiles were obtained and analyzed. A suitable macrodilution methodology based on a turbidimetric technique was adopted to produce inhibitory data used for characterizing microbial susceptibility against thymol at sub-MIC levels. Microbial growth was monitored through absorbance measurements at 420 nm as a function of contact time with the active compound. Moreover, for each tested microorganism, the noninhibitory concentration (NIC) and the MIC were quantified. Results prove that thymol can exert a significant antimicrobial effect on each phase of the growth cycle. The microbial susceptibility and resistance were found to be nonlinearly dose related. It is worth noting that significant biostatic effects were observed at sub-MIC levels.     

Effects of different vinification technologies on physico-chemical properties and antioxidant activity of ‘Falanghina’ and ‘Bombino bianco’ wines

The present research was aimed to compare the traditional white vinification with a combined cryomaceration–vinification in reductive conditions, by evaluating their effects on chemical and physical indices and on antioxidant compounds of ‘Falanghina’ and ‘Bombino bianco’, two wine grape cultivars of southern Italy. According to the results, the two cultivars showed similar behaviour in response to the same vinification protocol. The processing conditions mainly affected physico-chemical characteristics such as titratable acidity, pH, SO₂, tartaric and malic acids, phenolic concentrations, and antioxidant activity. Titratable acidity decreased with cryomaceration due to the tartrate precipitation, whereas the malic acid was greatly extracted from pulps and skins. In the reductive conditions applied, the SO₂ was protected by the presence of ascorbic acid; thus, the wines exhibited higher free/total SO₂ ratio. Cryomaceration combined with vinification in reductive conditions allowed a greater extraction of phenolic compounds from skins and, consequently, a greater antioxidant activity compared to the traditional winemaking. This behaviour was due to both the protection exerted by the low temperatures and the solubilisation of proanthocyanidins and flavans reactive with vanillin favoured by the high free SO₂ content.     

Mechanisms of the FMR1 Repeat Instability: How Does the CGG Sequence Expand?

A dynamic mutation in exon 1 of the FMR1 gene causes Fragile X-related Disorders (FXDs), due to the expansion of an unstable CGG repeat sequence. Based on the CGG sequence size, two types of FMR1 alleles are possible: “premutation” (PM, with 56-200 CGGs) and “full mutation” (FM, with >200 triplets). Premutated females are at risk of transmitting a FM allele that, when methylated, epigenetically silences FMR1 and causes Fragile X syndrome (FXS), a very common form of inherited intellectual disability (ID). Expansions events of the CGG sequence are predominant over contractions and are responsible for meiotic and mitotic instability. The CGG repeat usually includes one or more AGG interspersed triplets that influence allele stability and the risk of transmitting FM to children through maternal meiosis. A unique mechanism responsible for repeat instability has not been identified, but several processes are under investigations using cellular and animal models. The formation of unusual secondary DNA structures at the expanded repeats are likely to occur and contribute to the CGG expansion. This review will focus on the current knowledge about CGG repeat instability addressing the CGG sequence expands.     

The RRA‐ISA multiple access protocol with and without simple priority schemes for real‐time and data traffic in wireless cellular systems

A multiple access protocol, based on a Reservation Random Access (RRA) scheme, is derived for a wireless cellular network carrying real-time and data traffic. Given a TDMA framed channel and a cellular structure, the aim of the protocol is that of maximizing the one-step throughput over an entire frame. This is achieved by deciding on the access rights at the cell base station, which then broadcasts this information at the beginning of the frame. The decision is made on the basis of binary channel feedback information (collision/no collision) over the previous frames, as well as of long term averages of packet generation rates at the mobile stations, assuming independence in the presence of packets at the latter. The resulting protocol has therefore been termed Independent Stations Algorithm (ISA), and the overall scheme RRA-ISA. As in other RRA protocols, time constrained (e.g., voice) traffic operates in a dynamic reservation mode, by contending for a slot in the frame with the first packet of a burst, and then keeping the eventually accessed slot for the duration of the burst; packets of the time constrained traffic unable to access a slot within a maximum delay are dropped from the input buffer. No such constraint is imposed on data traffic. Together with the “basic” version of the access algorithm, three other variants are presented, which exploit three simple different priority schemes in the RRA-ISA “basic” structure, in order to give a prominence to the voice service. The aim of these variants is to improve the performance in terms of the maximum number of stations acceptable in the system, by slightly increasing the data packets delay. All the proposed schemes are analyzed by simulation in the presence of voice and data traffic. Several comparisons show a relevant performance improvement (in terms of data delay and maximum number of voice stations acceptable within a cell) over other protocols that use ALOHA as a reservation mechanism (RRA-ALOHA or PRMA schemes). This revised version was published online in June 2006 with corrections to the Cover Date.     

Extracellular pancuronium affects sodium current in chick embryo sensory neurones

1. The action of pancuronium on transmembrane sodium conductance was investigated in dorsal root ganglion neurones of chick embryos. The Na+ current was measured by use of the patch-clamp technique in whole-cell configuration. 2. Externally perfused pancuronium (50 microM to 1 mM) reversibly inhibited the current by a fast mechanism of action. Inhibition was concentration-dependent (with a half-effective dose of 170 microM) but not voltage-dependent. 3. The activation and inactivation kinetics of the Na+ current were estimated in pancuronium and in control solution by fitting experimental data with a Hodgkin-Huxley theoretical model. 4. The activation time constant tau m, at negative membrane voltages, was larger in the presence of pancuronium than in the control. In contrast, the inactivation time constant tau h was smaller during drug perfusion at membrane voltages < -10 mV. The steady-state inactivation h infinity was not affected by pancuronium. 5. These results suggest that pancuronium may reduce the sodium current by interacting with the sodium channels in both the resting and open states.     

Time-compressed video pictures for vision research

An interface is presented that uses time compression to allow static TV pictures (picked up by standard videoprocessing systems or synthesized by software) to be displayed at a rate of about 500 frames/s. Time compression of the video signal is achieved in two steps. The digitized video information is first loaded into the memory buffer or the device, the video memory. After completing a computer-aided loading cycle, the device is switched to the automatic reading mode, in which the video memory is read sequentially at a rate of 8 million words/s. Since the format of the picture is 128×128 pixels, the time compression factor, as compared to standard video signals, is about ten. The values of the device parameters (repetition rate, format, and gray tones number) were chosen to meet the requirements of a specific application in the field of vision research: the presentation of complex figures with high information content as stabilized images or, more generally, in the variable retinal feedback condition     

Comparison of measures of visual acuity in infants: Teller acuity cards and sweep visual evoked potentials

PURPOSE: This study compares the development of acuity in the same infants during one testing session using Teller acuity cards (TAC) and sweep visual evoked potentials (sVEP). We asked whether different testing methods in two centers would produce different developmental time courses. METHODS: Forty-eight infants were tested in two centers. The standard procedure for TAC was used. For sVEP acuity, the amplitude response curve derived from time-locked cortical activity was used to extrapolate to zero response, giving an acuity estimate for each infant. RESULTS: sVEP acuity was generally higher than TAC acuity. The rate of development was steeper for TAC than sVEP acuity with TAC starting at a much lower level. The ratio of sVEP to TAC acuity decreased exponentially with age reaching an asymptote of about 1.44 at 6 months. CONCLUSIONS: Results were indistinguishable between centers suggesting that comparison of acuity measures obtained using variations of these methods across centers is possible.     

Inhibition of human angiogenin by DNA aptamers: nuclear colocalization of an angiogenin-inhibitor complex

Specific ligands (aptamers) for angiogenin were selected from a 72-mer oligodeoxynucleotide library consisting of 28 randomized positions flanked by two constant regions of 22 residues each. From a starting pool of approximately 10(14) molecules, 19 angiogenin-binding ligands were obtained. Among them, two oligonucleotides showed significant inhibition of the ribonucleolytic activity of angiogenin with apparent Kis of 0.65 and 0.60 micro M, respectively. One of them was shortened on the basis of its secondary structure to provide a 45-mer oligonucleotide that retained much of the inhibitory properties of the parent molecule. It inhibits both the angiogenic and cell proliferative activities of angiogenin but does not interfere with its nuclear translocation in human endothelial cells. Importantly, the inhibitor is cotranslocated to the nucleus with angiogenin in a approximately 1:1 stoichiometric ratio. These results demonstrate that the inhibition of angiogenin-induced cell proliferation and angiogenesis by the oligonucleotide is due to suppression of the ribonucleolytic activity of angiogenin, an event that occurs most likely within the cell nucleus.     

ginSODA: massive parallel integration of stiff ODE systems on GPUs

The Journal of Supercomputing - Ordinary differential equations (ODEs) are a widespread formalism for the mathematical modeling of natural and engineering systems, whose analysis is generally...