Dummy humanoid robot simulating several trunk postures and abdominal shapes – Report of element technologies

Development of clothing in consideration of the shape and body function of a person with spinal cord injury is an important task. Then, a dummy robot with a deformation mechanism was developed in this study for evaluating the comfortable level of clothings. Specifically, a trunk joint mechanism and an abdominal mechanism that can realize various deformations of the abdominal area and various trunk poses were developed. The trunk joint mechanism was implemented in order to simulate the seated posture of persons with spinal cord injury. The abdominal deformation mechanism was implemented using linear actuators and rotating servomotors in order to simulate abdominal obesity of persons with spinal cord injury. Further, a tactile sensor system was developed for measuring the clothing pressure on the abdominal area and evaluating the comfort or discomfort of clothing.     

Affinity capture and elution/electrospray ionization mass spectrometry assay of phosphomannomutase and phosphomannose isomerase for the multiplex analysis of congenital disorders of glycosylation types Ia and Ib

We report a new application of affinity capture-elution electrospray mass spectrometry (ACESI-MS) to assay the enzymes phosphomannomutase (PMM) and phosphomannose isomerase (PMI), which when deficient cause congenital disorders of glycosylation CDG-type Ia and type Ib, respectively. The novel feature of this mass-spectrometry-based assay is that it allows one to distinguish and quantify enzymatic products that are isomeric with their substrates that are present simultaneously in complex mixtures, such as cultured human cell homogenates. This is achieved by coupled assays in which the PMM and PMI primary products are in vitro subjected to another enzymatic reaction with yeast transketolase that changes the mass of the products to be detected by mass spectrometry. The affinity purification procedure is fully automated, and the mass spectrometric analysis is multiplexed in a fashion that is suitable for high-throughput applications.     

Sequence analysis of small cryptic plasmids isolated from Selenomonas ruminantium S20

The degradation kinetics of five glutamine dipeptides in aqueous solution, i.e. glycyl-L-glutamine (Gly-Gln), L-alanyl-L-glutamine (Ala-Gln), L-valyl-L-glutamine (Val-Gln), L-leucyl-L-glutamine (Leu-Gln) and L-isoleucyl-L-glutamine (Ile-Gln), were studied. Stability tests were performed using a stability-indicating high-performance liquid chromatographic assay. Two different Ala-Gln degradation routes, i.e. the cleavage of a peptide bond and the deamination of an amide group, were observed. The degradation was adequately described by pseudo-first-order kinetics. The maximum stability of Ala-Gln was obtained at an approximate pH of 6.0. The pH-rate profile described by specific acid-base catalysis and hydrolysis by water molecules agreed with the experimental results. The activation energy of Ala-Gln at pH 6.0 was determined to be 27. 1kcal mol-1, and the shelf-life (90% remaining) at 25 and 40 degrees C was predicted to be 5.3 years and 7.1 months, respectively. The rate constants of the glutamine dipeptides were influenced by the N-terminal amino acid residue and decreased in the order: Gly-Gln, Ala-Gln, Leu-Gln, Val-Gln and Ile-Gln.     

Satellite direct broadcast

A brief history and commentary on the development of satellite direct broadcast (direct broadcast satellite, or DBS) to small user terminals is given, and the interrelations among the technical, economic, and political aspects are discussed. The fundamental technical problems in the transmission of video and audio directly to small user terminals are reviewed at a basic level, and points of particular interest relating to DBS are highlighted. The effects of improved transmission systems using multiplexed analog components and of high-definition television are related to the satellite transmission problems. The conflicting requirements of performance, compatibility with existing equipment, competition with other means of transmission, and vested political interests are pointed out. A reasonably complete summary of existing programs, both true DBS and near DBS, is given     

Oscillating thromboemboli within the extracranial internal carotid artery demonstrated by ultrasonography in patients with acute cardioembolic stroke

BACKGROUND: Gunshot wounds to the kidney have been managed traditionally by surgical exploration. Concerns over increased nephrectomy rates and unnecessary explorations have placed this method under scrutiny. Selective renal exploration based on solid clinical and radiographic criteria may be a safer alternative and deserves evaluation. METHODS: The medical records of 52 consecutive patients with renal gunshot wounds, who were managed between September 1994 and August 1995 by a protocol of selective exploration, were reviewed. Renal injuries were explored only if they involved the hilum or were accompanied by signs of continued bleeding. Main outcome measures were the numbers of kidneys lost, as well as the morbidity and mortality related to the management of the renal injury. RESULTS: Three patients died from associated injuries shortly after admission. Fifteen patients suffered complications but only two had complications directly associated with the renal injury. Thirty-two patients underwent renal exploration and 17 of them required nephrectomy for major renovascular or parenchymal trauma (grade IV and V). Renal exploration was successfully avoided in the remaining 20 patients. No kidneys were lost unnecessarily as a result of this policy. CONCLUSION: Mandatory exploration of all gunshot wounds to the kidney is not necessary. Injuries that produce stable peripheral haematomas do not require exploration.     

In vitro parathyroid hormone release in patients with secondary hyperparathyroidism

BACKGROUND: The purpose of this study was to determine the precise endocrine characteristics of parathyroid function in secondary hyperparathyroidism (sHPT). METHODS: We examined the effects of extracellular ionized calcium (Ca2+) varying from 0.5 to 2.0 mM on parathyroid hormone (PTH) release in parathyroid cell suspensions using a mid-regional PTH assay. Cells were obtained from 26 patients with sHPT who were divided into two groups according to the type of hyperplasia they exhibited, either nodular (n = 16) or diffuse (n = 10). For comparison, we also analyzed data from nine patients with primary hyperparathyroidism (pHPT; adenomas). RESULTS: Significant in vitro suppression of PTH release by Ca2+ was observed in the majority of subjects, regardless of the histologic abnormality. The pHPT group exhibited no significant relationship between clinical and in vitro data. In contrast, in the sHPT group (taken as a whole), suppression of PTH release by Ca2+ exhibited a plateau at a total serum calcium concentration of 2.5 mmol/L, and a parathyroid gland weight of 2 g. CONCLUSIONS: These findings suggest that there is a curvilinear relationship in sHPT, but not pHPT, between the in vitro calcium sensitivity of parathyroid cells and total serum calcium, as well as gland weight. The in vitro calcium sensitivity in sHPT remains constant when the total serum calcium concentration exceeds 2.5 mmol/L, or when the gland weight exceeds 2 g.     

Portal-hepatic venous shunt through a portal aneurysm complicated by hepatic encephalopathy and pulmonary hypertension

We report a rare case of portal-hepatic venous shunt through an enormous portal aneurysm complicated by pulmonary hypertension. A 66-year-old woman was admitted to our hospital for hepatic encephalopathy. Chest roentgenography revealed pulmonary hypertension. Computed tomography and ultrasound examination demonstrated a shunt between the portal and hepatic veins through an enormous portal aneurysm. The diagnoses of portal-hepatic venous shunt and pulmonary hypertension were confirmed by hepatic venous catheterization and cardiac catheterization. Pulmonary hypertension might result from the effects of vasoconstrictive agents, which should be metabolized by the liver in normal subjects, passing through the intrahepatic shunt into the lung.     

Continuous reactor system of monosized colloidal particles

A reactor system, which continuously hydrolysed the metal alkoxide in an alcohol solution, was designed using an electromagnetic stirrer and an ageing tube. Several monosized colloidal particles were produced by this reactor system, which had high reproducibility and reliability for long-term production. The relation between powder characteristics and experimental parameters such as reagent concentration, mixing rate, ageing time, temperature, was investigated. These parameters had an effect on the particle size, size distribution, morphology and state of agglomeration. It is possible to control the particle size to between 0.1 and 1.0 μm by varying the experimental conditions. A narrower size distribution of powders was obtained by using an electromagnetic stirrer with greater flow rate. Physical and chemical properties of monosized colloidal particles obtained by this reactor were comparable to those of monosized colloidal particles obtained by the batch process.     

The dependency of the crack growth rate on the loading pattern and temperature in stress corrosion cracking of strain-hardened 316L stainless steels in a simulated BWR environment

The stress corrosion cracking (SCC) growth rate of a warm-rolled (WR) 316L stainless steel contoured double cantilever (CDCB) specimen was measured in high purity water at various temperatures and under various loading patterns. An alternating current potential drop (ACPD) technique was used to monitor the crack growth kinetics throughout the tests. The fracture surface exhibited typical intergranular SCC characteristics. Depending on the test conditions, three kinds of crack growth kinetics, i.e., increasing with time then becoming steady, being constant during the whole period, decreasing with time then becoming steady, were identified and are described. The steady state crack growth rate (CGR) values are used to quantify the effects of the loading pattern and the environmental temperature. A moderate increase in the crack growth rate was encountered by employing periods of unloading and reloading to form a trapezoidal loading pattern and the enhancement factor was found to depend on the holding time and the times for unloading and reloading. It was found that the crack growth is thermally activated; however, the apparent activation energy is not constant but seems to be greater at higher temperatures. Several types of temperature-dependent crack growth kinetics are proposed based on the rate-determining step for the crack growth. The present experimental results can be rationalized by considering multiple element processes such as aqueous mass transport and solid-state mass transport in the crack growth. The cracking mode, the temperature dependence of the crack growth rate, and the transient crack growth behavior for WR 316L SS after changing the environmental temperature are quite similar to those for a cold-worked(CW) 316L SS tested in the same environment, despite their different absolute crack growth rate values. The effect of yield strength on CGR is more significant at lower temperatures and the apparent activation energy for the crack growth rate seems to be lower in the material with a higher yield strength. Time-lag crack growth behavior was found at points during several test steps on WR 316L SS, for example, just after in situ pre-cracking and after increasing or decreasing the temperature, which is quite consistent with the results obtained with CW 316L SS. The importance of in situ monitoring of the crack growth for obtaining steady state crack growth rates is emphasized, especially for those steps for which a nonlinear crack growth period occurs after changing the test condition.