NEUTRAL AND ALKALINE MUSCLE PROTEASES OF MARINE FISH AND INVERTEBRATES A REVIEW

Muscle proteases active at neutral and alkaline pH's include calcium-activated neutral proteases, heat-activated thiol proteases, serine proteases, and metallo-proteases. They participate to different extents in postmortem degradation of fish muscle myofibrillar and scaffold proteins. Their activity in fish, the presence of endogenous activators and inhibitors, pH, and temperature. Recent studies indicate that neutral and alkaline proteases have more impact on the postmortem deterioration in quality of fish muscles than the cathepsins active at acid pH. Significant quality losses are caused by enzymatic collagen degradation in raw tissues and by heat-activated enzymes in fish gels.     

Antibacterial activity of sourdough lactic acid bacteria: isolation of a bacteriocin-like inhibitory substance fromLactobacillus sanfranciscoC57

A total of 232 strains from nine species ofLactobacillusisolated from sourdoughs were screened for antagonistic activity against sourdough-related micro-organisms. Seventy-seven strains on agar medium and 52 in culture supernatants, re-adjusted to pH 6.5 and catalase-treated, showed antagonistic activity. The activities were species and strain specific showing different spectra of inhibition against sourdough lactobacilli. All the strains were inhibitory toBacillus subtilisbut not against sourdough yeasts and moulds.Lactobacillus sanfranciscoandLactobacillus plantarumstrains had the largest inhibitory spectrum. All the antimicrobial compounds produced from strains of different species contained a protein moiety and were differently sensitive to different proteinases. A bacteriocin-like inhibitory substance, which was heat-stable (100°C for 20 min), insensitive to lipase and α-amylase, of a protein nature, with an inhibitory spectrum centred about lactic acid bacteria and a bactericidal or bacteriolytic mode of action was isolated fromLb. sanfranciscoC57. The antimicrobial substance also inhibitedListeria monocytogenes, and was mainly produced in the stationary phase of growth and at pH 4.0–5.0.Lb. sanfranciscoC57 variants, which did not contain the nativec. 17 kbp plasmid, maintained their antagonistic activity, therefore, the gene encoding for the bacteriocin-like inhibitory substance fromLb. sanfranciscoC57 is chromosomally located.     

Epstein-Barr virus recombinants from overlapping cosmid fragments

Five overlapping type 1 Epstein-Barr virus (EBV) DNA fragments constituting a complete replication- and transformation-competent genome were cloned into cosmids and transfected together into P3HR-1 cells, along with a plasmid encoding the Z immediate-early activator of EBV replication. P3HR-1 cells harbor a type 2 EBV which is unable to transform primary B lymphocytes because of a deletion of DNA encoding EBNA LP and EBNA 2, but the P3HR-1 EBV can provide replication functions in trans and can recombine with the transfected cosmids. EBV recombinants which have the type 1 EBNA LP and 2 genes from the transfected EcoRI-A cosmid DNA were selectively and clonally recovered by exploiting the unique ability of the recombinants to transform primary B lymphocytes into lymphoblastoid cell lines. PCR and immunoblot analyses for seven distinguishing markers of the type 1 transfected DNAs identified cell lines infected with EBV recombinants which had incorporated EBV DNA fragments beyond the transformation marker-rescuing EcoRI-A fragment. Approximately 10% of the transforming virus recombinants had markers mapping at 7, 46 to 52, 93 to 100, 108 to 110, 122, and 152 kbp from the 172-kbp transfected genome. These recombinants probably result from recombination among the transfected cosmid-cloned EBV DNA fragments. The one recombinant virus examined in detail by Southern blot analysis has all the polymorphisms characteristic of the transfected type 1 cosmid DNA and none characteristic of the type 2 P3HR-1 EBV DNA. This recombinant was wild type in primary B-lymphocyte infection, growth transformation, and lytic replication. Overall, the type 1 EBNA 3A gene was incorporated into 26% of the transformation marker-rescued recombinants, a frequency which was considerably higher than that observed in previous experiments with two-cosmid EBV DNA cotransfections into P3HR-1 cells (B. Tomkinson and E. Kieff, J. Virol. 66:780-789, 1992). Of the recombinants which had incorporated the marker-rescuing cosmid DNA fragment and the fragment encoding the type 1 EBNA 3A gene, most had incorporated markers from at least two other transfected cosmid DNA fragments, indicating a propensity for multiple homologous recombinations. The frequency of incorporation of the nonselected transfected type 1 EBNA 3C gene, which is near the end of two of the transfected cosmids, was 26% overall, versus 3% in previous experiments using transfections with two EBV DNA cosmids. In contrast, the frequency of incorporation of a 12-kb EBV DNA deletion which was near the end of two of the transfected cosmids was only 13%.(ABSTRACT TRUNCATED AT 400 WORDS)     

METHODS FOR BRAZING CERAMIC AND METAL-CERAMIC JOINTS

Owing to improved manufacturing processes technical ceramics on the basis of oxide as well as nonoxide ceramics have gained an increasing technical potential. Among other joining techniques brazing has proved to be the most flexible joining process that can easily be adapted to various ceramic-metal-combinations. In order to induce a wetting reaction there are two different approaches. The more complicated process encircles a pre-metallization process and a subsequent brazing process. The less sophisticated process is the 'active brazing process' where special brazing alloys are employed that are able to wet ceramic base materials. Although both joining processes are very flexible there are restrictions regarding the filler metals to be used, the premetallization process and the actual (active-) brazing process.     

Interactions in the complement-mediated lysis of blood group AB erythrocytes sensitized simultaneously with anti-A and anti-B monoclonal antibodies

The lysis of group AB erythrocytes by human complement was studied by different anti-A and anti-B IgM monoclonal antibodies (mabs) in a 51Cr-release assay. The concentration of membrane-bound immunoglobulin was detected by ELISA, and the amount of C1q and C3 bound to sensitized red cells was measured by using purified, 125I-labelled molecules. We have demonstrated that there is an exponential relationship between the concentration of the sensitizing IgM mabs and C1q binding to the sensitized AB cell. The efficiency of binding was related to the number of antibodies bound; thus, anti-A sensitized cells bound 3-6 times more C1q than anti-B sensitized cells did. AB cells, on the other hand, bound similar amounts of C3 whether anti-A or anti-B was present. The lytic efficiencies of the various IgM mabs during short incubation times were different, suggesting that the complement activation rates vary widely with different antibodies on the AB cell membrane. The binding of C1q to an antibody-sensitized target activates a cascade, whose components may migrate away from the sensitizing antibody; interactions between the activation processes generated by the anti-A and anti-B antibodies may thus occur. Choosing appropriate pairs of anti-A and anti-B mabs for the simultaneous sensitization of AB cells has indeed resulted in stimulation in some and inhibition in other combinations of mabs. It is suggested that stimulation is observed when the activated intermediates are produced in excess, whereas inhibition occurs when a shortage of activated intermediates prevents mutual utilization.     

Power engineering industry interaction at Clemson University

The authors present a successful model of industry/university cooperation in establishing a strong power system curriculum in both the graduate and undergraduate level. Numerous long-term and short-term research projects have been developed to satisfy the university mission and to tackle challenging problems facing the power industry. A unique structure for the Clemson University Electric Power Research Association (CUEPRA) has been established to promote electric power system research and to meet the need for a working communication link between the power industry and the academic community. The power industries involvements in the power program at Clemson University and the strategic improvements that have been accomplished in research and education are outlined     

Neuramide stimulates adrenocorticotropin but not prolactin release from rat pituitary

Neuramide (NMD), a substance found in crude preparations of porcine stomach extract, is a viral inhibitor that also has putative immunostimulatory effects. The effects of NMD on stress-hormone (ACTH and prolactin-PRL) release were assessed in in vivo and in vitro studies. In the former, blood levels of corticosterone and PRL were measured in NMD-treated male rats. In vitro experiments were performed to evaluate the effects of NMD and three of its fractions (obtained with high performance liquid chromatography) on ACTH and PRL release from perfused rat pituitary slices. NMD increased plasma corticosterone levels in vivo and produced dose-dependent increases in in vitro pituitary release of ACTH. No effects on PRL secretion were observed in vivo or in vitro. The stimulatory effects on ACTH release were caused by the NMD fraction with a molecular weight of > 5000 < 10000 Da.     

Setting the record straight.

Replies to comments by H. W. Marsh and L. A. Roche (see record 1997-43129-003) on the author's article (Educational Policy Analysis Archives, 1997) on student evaluation and academic freedom. The author expresses his dismay that Marsh and Roche misrepresented (not misinterpreted) the article without documenting their misrepresentation. A documented response to this undocumented misrepresentation is provided. (PsycINFO Database Record (c) 2010 APA, all rights reserved)