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61.
This paper examines governance processes shaping the experiences of neighbourhood restructuring-induced residential relocation in Housing Market Renewal (HMR) areas in England. Since the 1950s and 1960s, residential relocation has been examined as a matter of social and political debate, especially in gentrification studies focusing mainly on negative residential relocation outcomes long after the process was over. This paper argues that such a focus had led researchers to ignore subtle, practical dimensions of relocation delivery and the causal relationships between these and often very diverse relocation experiences. Based on the HMR case, the study shows that residential relocation in a differentiated polity is delivered by complex networks of actors and that residential relocation outcomes are the result of cooperation or non-cooperation of network members. Key innovation rests in devising a theoretical vehicle that shows how governance has a profound impact on relocation delivery and residential relocation experiences.  相似文献   
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63.
SP1 protein-based nanostructures and arrays   总被引:1,自引:0,他引:1  
Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.  相似文献   
64.
Here we present a set of resources (bacterial expression plasmids and antibodies) for the interrogation of proteins involved in yeast MAPK signalling. We constructed bacterial protein expression plasmids for 25 proteins involved in MAPK signalling in budding yeast. From these constructs we expressed and purified proteins and generated rabbit polyclonal antibodies against 13 proteins in the pheromone MAPK pathway. We verified the specificity of the antibodies and employed them to follow pathway proteins in cells stimulated with pheromone. We show that these reagents can be used to detect pheromone‐induced post‐translational modifications and changes in the oligomeric state of pathway proteins. In addition to recognizing their target proteins in Saccharomyces cerevisiae, these antibodies allow the detection of predicted orthologues in the distant evolutionary relatives Kluyveromyces lactis and Schizosaccharomyces pombe. These antibodies are new tools for investigating MAPK signalling in model yeast species and may be useful for studying MAPK signalling in higher eukaryotes. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   
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66.
Suicidal, psychiatric, and normal adolescent girls and boys, ages 15–17 yrs, participated in a study on five types of death fears. A mixed within-between multivariate analysis of variance (5 factors?×?3 groups) yielded significant interactive effects indicating that there were different profiles of fears among the three groups: Relative to the other groups, suicidal adolescents displayed little differentiation between facets of fear, with differences in the magnitude of fear limited to the intrapersonal facets. Correlates of fear varied between groups: Fear was positively correlated with suicidality in normal Ss, negatively correlated with suicidality among suicidal ones, and unrelated to suicidality in the psychiatric group. It is suggested that fear of death is processed and experienced differently by suicidal and nonsuicidal adolescents to serve as a facilitator or inhibitor of suicidal behavior. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
67.
The ability to imitate facial expressions was examined in 26 newborns. Each subject observed a model showing tongue protrusion or a happy, sad, or suprised face. The frequencies of reproduction of a modeled act were compared with the average frequencies of the act during periods when other actions were modeled. A trials-to-criterion design was used. When infants observed the emotional facial expressions (happy, sad, surprise), they often responded by opening their mouths or showing lip pouts, but did not show imitative-like matching of these modeled expressions. However, when tongue protrusion was modeled, the infants did reproduce the modeled gesture. These data raise the question of whether the infants' responses to modeled facial expressions reflect true imitation, stimulus-evoked elicitation, or a stereotyped "facial gesture." (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
68.
We proposed that help-seeking perceptions and behaviors will be more adaptive under salient task goals relative to ego achievement goals. A total of 159 2nd- and 6th-grade Israeli children could request help as they worked on difficult puzzles in either a task or an ego goal condition. As predicted, children were more likely to request help and to explain help avoidance as guided by strivings for independent mastery in the task-focus condition. In contrast, more children in the ego-focus condition explained help avoidance in terms of masking incapacity. Skill level moderated help seeking only in the ego-focus condition, wherein requests for help were more frequent at intermediate than at both high and low skill levels. The results clarify the role of motivational factors in promoting or undermining academic help seeking and can help resolve theoretical controversy and inconsistent empirical findings concerning the relation between competence and help seeking. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
69.
Three patients with thyrotoxicosis are described, in whom the presenting symptom was severe cardiac pain at rest or on effort and who were admitted to hospital with suspected or proven myocardial infarction. All patients were studied by selective coronary arteriography and left ventriculography after thyroid function tests which confirmed thyrotoxicosis. There was no demonstrable disease of the major coronary arteries in any of the patients, yet myocardial infarction and left ventricular aneurysm were shown to be present in 1, and there was definite electrocardiographic evidence of ischaemia in all 3. In addition, under stress the myocardium of all 3 patients produced lactate. It is recommended that thyrotoxicosis be seriously considered in the differential diagnosis of cardiac pain, particularly in younger women. The cause of the pain seems related to the cellular effects of thyrotoxicosis on the myocardium and current views of these effects are summarised. Of the 3 patients, 1 died suddenly 6 months after becoming euthyroid, indicating that the disease may not be as benign as expected. A guarded prognosis and continued medical follow-up are recommended when thyrotoxicosis presents with angina pectoris even when normal coronary arteries have been demonstrated.  相似文献   
70.
A newly designed mixer, the Alberta polymer asymmetric minimixer (APAM), was compared to the MiniMAX molder with flow simulation and flow visualization techniques to evaluate the performance of the mixers. The APAM has a unique, asymmetric design consisting of a varying clearance between the rotor blade tips and the cup wall, which enables the material to be squeezed, stretched, and kneaded in high‐shear and converging zones. Flow simulation showed that substantial folding and axial movement occurred in this mixer and that the pressure and velocity profiles exhibited high values at the rotor tip with the smallest rotor tip/cup clearance. In contrast, the MiniMAX molder had very simple flow patterns, which were insufficient to induce good dispersive and distributive mixing. These results concurred with those from an earlier work that studied the structure of blends and nanocomposites processed in the APAM compared to other polymer processing equipment. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 97: 136–142, 2005  相似文献   
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