Effects of dissolved carbonates and carboxylates on the sorption of thiuram disulfide pesticides on humic acids and model surfaces

The sorption of a hydrophobic pesticide, thiram, on humic acid (HA) occurs via a specific pH-dependent binding of thiram at the deprotonated carboxylates of humic acid, forming a species thiram-[HACOO-] with K = 0.69. Similarly, thiram was sorbed by two model polycarboxylate-{SiO2COOH} materials via the formation of a surface species thiram-{SiO2COO-} with K = 0.45 between thiram and the eprotonated carboxylates grafted on SiO2 particles. In all cases, allowance of presence of bicarbonate at natural concentration caused severe inhibition of thiram's sorption. Oxalate and formate mimic the inhibitive effect of bicarbonate. Theoretical fit of the data showed that the inhibitive effect of HCO3- is due to the formation of the anionic species [thiram-HCO3](-1) (with K = 0.90) which is water soluble and competes with the bound species thiram-{HACOO-}. The same phenomena were observed for the sorption of disulfiram. The specific interaction phenomena reported here bear relevance to the sorption properties of thiram and disulfiram on real soils and, therefore, may determine their environmental fate.     

Simulation of façade and envelope design options for a new institutional building

This paper presents a simulation case study of façade and envelope preliminary design options for the new Engineering building of Concordia University in Montreal. A major principle of the analysis was to create a high quality building envelope in order to optimally control solar gains, reduce heating and cooling energy demand and reduce electricity consumption for lighting, while at the same time maintain a comfortable and pleasant indoor environment. The stated approach of the design team was to aim for an energy-efficient building, employing innovative technologies and integrating concepts such as daylighting and natural ventilation. Detailed energy simulations were therefore performed from the early design stage, in order to present recommendations on the choice of façade, glazings, shading devices, lighting control options, and natural ventilation. Integrated thermal studies, a daylighting analysis and the impact of the above on HVAC system sizing were considered. Simulation results showed that, using an optimum combination of glazings, shading devices and controllable electric lighting systems, the energy savings in perimeter spaces can be substantial. Perimeter heating could be eliminated if a high performance envelope is used. The building is currently being commissioned.     

Calcium chloride irrigation influence on yield,calcium content,quality and shelf‐life of the white mushroom Agaricus bisporus

Seven CaCl₂ irrigation treatments (0.15, 0.25, 0.35, 0.45, 0.55, 0.75 and 1.00%) were applied to the Agaricus bisporus white strain A15 (Sylvan) in order to evaluate, in comparison with the water‐irrigated crop, their effect on production characteristics, ie yield, sporophore number and size mix, average mushroom weight as well as pileus and stipe Ca content. In addition, initial mushroom colour and texture in three successive flushes and their variations during post‐harvest storage for 8 days at 4 °C and for 2 days at 18 °C were determined. Statistical analysis of data revealed that crop yields were not affected by the individual treatments, whereas the mushroom number was reduced and the average sporophore weight was enhanced. A strong positive correlation was established between CaCl₂ dosage and Ca content of fresh mushrooms, which increased considerably in the third flush by accumulating in both pileus and stipe parts (the former containing less Ca than the latter) and presented a maximum for the 0.75 and 1.00% CaCl₂ treatments. At harvest, colour of second‐ and third‐break mushrooms was significantly improved by all CaCl₂ treatments. This beneficial effect was more pronounced after 8 days of cold storage and during shelf‐life in sporophores treated with 0.35, 0.75 and 1.00% CaCl₂. No consistent correlation was found between Ca content and texture of fresh and stored mushrooms. However, although softer mushrooms were recorded at harvest in most CaCl₂‐irrigated crops, 0.25, 0.75 and 1.00% concentrations appeared to retard mushroom softening, promoting firmness at the 8th day of cold storage and during shelf‐life. © 2001 Society of Chemical Industry     

A monolithic silicon optoelectronic transducer as a real-time affinity biosensor

An optical real-time affinity biosensor, which is based on a monolithic silicon optoelectronic transducer and a microfluidic module, is described. The transducer monolithically integrates silicon avalanche diodes as light sources, silicon nitride optical fibers, and p/n junction detectors and efficiently intercouples these elements through a self-alignment technique. The transducer surface is hydrophilized by oxygen plasma treatment, silanized with (3-aminopropyl)triethoxysilane and bioactivated through adsorption of the biomolecular probes. The use of a microfluidic module allows real-time monitoring of the binding reaction of the gold nanoparticle-labeled analytes with the immobilized probes. Their binding within the evanescent field at the surface of the optical fiber causes attenuated total reflection of the waveguided modes and reduction of the detector photocurrent. The biotin-streptavidin model assay was used for the evaluation of the analytical potentials of the device developed. Detection limits of 3.8 and 13 pM in terms of gold nanoparticle-labeled streptavidin were achieved for continuous- and stopped-flow assay modes, respectively. The detection sensitivity was improved by silver plating of the immobilized gold nanoparticles, and a detection limit of 20 fM was obtained after 20-min of silver plating. In addition, two different analytes, streptavidin and anti-mouse IgG, were simultaneously assayed on the same chip demonstrating the multianalyte potential of the sensor developed.     

Promising Biomarkers in Head and Neck Cancer: The Most Clinically Important miRNAs

Head and neck cancers (HNCs) comprise a heterogeneous group of tumors that extend from the oral cavity to the upper gastrointestinal tract. The principal etiologic factors for oral tumors include tobacco smoking and alcohol consumption, while human papillomavirus (HPV) infections have been accused of a high incidence of pharyngeal tumors. Accordingly, HPV detection has been extensively used to categorize carcinomas of the head and neck. The diverse nature of HNC highlights the necessity for novel, sensitive, and precise biomarkers for the prompt diagnosis of the disease, its successful monitoring, and the timely prognosis of patient clinical outcomes. In this context, the identification of certain microRNAs (miRNAs) and/or the detection of alterations in their expression patterns, in a variety of somatic fluids and tissues, could serve as valuable biomarkers for precision oncology. In the present review, we summarize some of the most frequently studied miRNAs (including miR-21, -375, -99, -34a, -200, -31, -125a/b, -196a/b, -9, -181a, -155, -146a, -23a, -16, -29, and let-7), their role as biomarkers, and their implication in HNC pathogenesis. Moreover, we designate the potential of given miRNAs and miRNA signatures as novel diagnostic and prognostic tools for successful patient stratification. Finally, we discuss the currently ongoing clinical trials that aim to identify the diagnostic, prognostic, or therapeutic utility of miRNAs in HNC.     

A comparative presentation of implementation of ISO 22000 versus HACCP and FMEA in a small size Greek factory producing smoked trout: a case study

ISO 22000 is the new standard bound to replace HACCP on issues related to food safety. Although several companies, especially the big ones, have either implemented or are on the point of implementing ISO 22000, there are many others which are rather timid and/or reluctant to implement it. The main reason behind that is the lack of information and the fear that the new standard is too demanding in terms of bureaucratic work. This paper aims at making a comparative presentation of how the two systems can be applied to a small smoked salmon producing company thereby facilitating the emergence of the differences. The main difference is that in ISO 22000 systems like Good Manufacturing Practice and Good Hygiene Practice are prerequisites thus leading to lower number of CCPs. In this case study for example, the number of CCPs dropped from eight (8) in HACCP to four (4) in ISO 22000. Furthermore, the Failure Mode and Effect Analysis was applied to the smoked trout manufacturing process in an attempt to calculate quantitatively the Risk Priority Number (RPN) and to find out whether it can be effectively correlated to ISO 22000 and/or HACCP. RPN was found to be higher than 130 for eight steps, in close agreement with HACCP, thereby indicating that corrective actions will have to be undertaken.     

The anticoagulant activity of enoxaparin sodium during on-line hemodiafiltration and conventional hemodialysis

To study and compare the anticoagulant activity of enoxaparin sodium during on-line hemodiafiltration (OL-HDF) and conventional hemodialysis (C-HD). Enoxaparin was administered as an anticoagulant to 21 hemodialysis patients at the beginning of a single 4-hour OL-HDF session as an intravenous bolus dose of 80 mg/kg. On-line hemodiafiltration was performed using a high-flux polyester polymer alloy dialyzer and a total of 18 L replacement fluid (session A). One week later, the study was repeated in the same patients during a single 4-hour session of C-HD using a low-flux polysulfone dialyzer (session B). Blood samples for the measurement of Hb, blood urea and nitrogen (BUN), activated partial thromboplastin time (APTT), and anti-Xa levels were taken before each study session and 5-minute postdialysis. In 13 more patients, the same study was performed during OL-HDF using a high-flux polysulfone dialyzer (session C). No differences were found between sessions A, B, and C when predialysis values for Hb, BUN, APTT, and anti-Xa were compared. The mean postdialysis APTT and anti-Xa values were 32.5±3.8 seconds and 0.19±0.11 IU/mL, respectively, in session A, 39.0±5.0 seconds and 0.71±0.17 IU/mL in session B, and 33.8±3.1 seconds and 0.35±17 IU/mL in session C (A vs. B, P<0.0001, for both parameters, A vs. C, P<0.003 for anti-XA, and B vs. C, P<0.005, for both parameters). The anticoagulant activity of enoxaparin sodium is decreased significantly during a 4-hour OL-HDF session compared with to a similar session of C-HD. The degree of the reduction seems to depend on the dialyzer's membrane.