Barcode High Resolution Melting (Bar-HRM) analysis for detection and quantification of PDO “Fava Santorinis” (Lathyrus clymenum) adulterants

Legumes considered as one of the most important crops worldwide. Due to high price as a PDO product, commercial products of “Fava Santorinis” are often subjected to adulterations from other legume products coming from other Lathyrus or Vicia and Pisum species. Using plant DNA barcoding regions (trnL and rpoC) coupled with High Resolution Melting (Bar-HRM) we have developed a method allowing us to detect and authenticate PDO “Fava Santorinis”. Bar-HRM proved to be a very sensitive tool able to genotype Lathyrus and its closed relative species and to detect admixtures, being sensitive enough to as low as 1:100 of non-“Fava Santorinis” in “Fava Santorinis” commercial products. In conclusion, Bar-HRM analysis can be a faster, with higher resolution and cost effectiveness alternative method to authenticate PDO “Fava Santorinis” and to quantitatively detect adulterations in “Fava Santorinis” with other relative commercial “Fava” food products.     

Mesh deformation-based multi-tissue mesh generation for brain images

Multi-tissue meshing is necessary for the realistic building of a biomechanical model of the brain, which has been widely used in brain surgery simulation, brain shift, and non-rigid registration. A two step multi-tissue mesher is developed. First, a coarse multi-tissue mesh is generated by redistributing labels of a body-centered cubic (BCC) mesh. Second, all the surfaces of the submeshes are deformed to their corresponding tissue boundaries. To deform the mesh, two strategies are developed. One is based on a point-based registration (PBR) and the other is based on a robust point matching (RPM). The PBR method explicitly calculates the correspondence, which takes both smoothing and quality into account, then resolves the displacement vector by minimizing an energy function. Unlike PBR method, RPM does not require the correspondence between the source points and the target points to be known in advance. To simultaneously resolve the displacement vector and the correspondence, the Expectation and Maximization optimization is employed to alternately estimate the correspondence and the displacement vector. To effectively cope with outliers, least trimmed square, a robust regression technique, is employed to correct the regression bias induced by outliers. Both methods are effective in deforming the multi-tissue mesh. However, the PBR method favors quality and smoothing, and the RPM method favors fidelity. The resulting mesh is characterized by its flexible control of four mesh properties: (1) tissue-dependent resolution, (2) fidelity to tissue boundaries, (3) smoothness of mesh surfaces, and (4) element quality. Each mesh property can be controlled on a tissue level. Our experiments conducted on synthetic data, clinic MRI, visible human data, and brain atlas effectively demonstrate these features of this multi-tissue mesher.     

Comparing hybrid learning with traditional approaches on learning the Microsoft Office Power Point 2003 program in tertiary education

The purpose of this study was to determine the effectiveness of a hybrid learning approach to deliver a computer science course concerning the Microsoft office PowerPoint 2003 program in comparison to delivering the same course content in the form of traditional lectures. A hundred and seventy-two first year university students were randomly assigned into two teaching method groups: traditional lecture instruction (TLI) and hybrid lecture instruction (HLI). Each group received six 95-min periods of instruction divided into 4 sections: a) 5-min brief outline of the key learning points, b) 40-min lecture on general knowledge c) 45-min constructivist-inspired learning activities and d) 5-min summary on key learning points. In the beginning and the end of this study students completed a 17-item multiple choice knowledge test. Two-way analysis of variances (ANOVA), with repeated measures on the last factor, were conducted to determine effect of method groups (TLI, HLI) and measures (pre-test, post-test) on knowledge test. The measures main effect was significant, as well as the groups x measures interaction effect. Two independent-samples t test were conducted to follow up the significant interaction. Differences in mean ratings of knowledge performance between the two teaching groups were not significantly different at first measure, while the TLI method group yielded a significantly lower mean rating at second measure. The findings indicated that HLI approach might be a superior option for undergraduate students on learning the Microsoft office PowerPoint 2003 program.     

Room‐Temperature Phase Demixing in Bulk Heterojunction Layers of Solution‐Processed Organic Photodetectors: the Effect of Active Layer Ageing on the Device Electro‐optical Properties

Herein, we address the reduction in the external quantum efficiency (EQE) of solution‐processed organic photodetectors caused by the room temperature phase demixing of components in the composite material of the photoactive layer. The reduction takes place under ambient conditions and after the completion of device fabrication. As a model system, we study photoactive blend films that consist of the electron acceptor N,N’‐bis(alkyl)‐3,4,9,10‐perylene tetracarboxylic diimide) (PDI) and the electron donor polymer poly(9,9’‐dioctylfluorene‐co‐benzothiadiazole) (F8BT). The ambient ageing of these photo­active layers is a consequence of the PDI component segregation; however, the final PDI domain size remains smaller than the resolution limit of optical microscopy. We find that the photophysical properties of the aged F8BT:PDI layer and the EQE of the aged device are significantly altered. The fabrication of F8BT:PDI layers from solvents of increasing boiling point allows for the spectroscopic monitoring of the ageing‐induced phase segregation (a‐PSG) process. For each solvent used, the extent of a‐PSG is correlated with the PDI dispersion in the F8BT matrix as received immediately after layer deposition. The tendency for room temperature phase demixing becomes stronger as PDI is more finely dispersed in the freshly spun F8BT:PDI layer. The evolution of the room temperature phase segregation of PDI has a negative impact on the photophysical processes that are essential for charge photogeneration in the F8BT:PDI photoactive layer.     

Design,construction and performance of a portable handheld electrohydrodynamic multi-needle spray gun for biomedical applications

Electrohydrodynamic (EHD) processing has attracted substantial interest in the technological and pharmaceutical sectors in recent years. Given the complexity of the process, exploring new ideas for EHD electrospraying and electrospinning delivery is a challenge. In this article, the design, construction and testing of a portable handheld EHD multi-needle device are described to produce multifunctional particles and fibers. Solid and encapsulated polymer particles and fibers were generated in order to study the performance of the device. The intrinsic properties of the feed solution/suspension and the processing conditions were adjusted to ensure robustness of the process and give uniform and reproducible products, with diameters ranging from the sub-micrometer scale to a few micrometers. These products have a broad range of applications in many advanced industrial sectors e.g. drug delivery systems, wound dressing patches, low calorie food products and cosmetics.     

Evaluating the combined effect of water activity,pH and temperature on ochratoxin A production by Aspergillus ochraceus and Aspergillus carbonarius οn culture medium and Corinth raisins

The objectives of this study were: (a) to evaluate the potential of the ELISA method in the determination of the produced OTA by Aspergillus ochraceus and Aspergillus carbonarius in malt extract agar (MEA) at different pH (3.9, 5.1, 5.9, 6.8), water activity (a_w) (0.87, 0.93, 0.99), and temperature (10, 15, 20, 25, 30, 40 °C) levels, providing a rapid screening for the optimum and marginal conditions of OTA production, (b) to comparatively evaluate the performance of ELISA and HPLC method, and (c) to evaluate the ability of A. ochraceus to produce OTA in rehydrated Corinth raisins during storage for 36 days. Two independent experiments were carried out to estimate OTA production on MEA and Corinth raisins. The produced OTA was evaluated qualitatively by the ELISA method and selected cases were verified by HPLC. The levels of OTA decreased with water activity, whereas pH seemed to have no specific effect. Furthermore, A. ochraceus produced maximum amounts of OTA on raisins at the 24th day of incubation, indicating that the endogenous microflora may restrictively affect OTA production. The knowledge of optimal and marginal levels of ecological factors in order to optimise post-harvest and storage of food products may significantly affect the production of OTA. Moreover, endogenous microflora of certain foodstuffs may cause OTA detoxification and consequently reduction of OTA levels; a fact that has to be taken into account in food commodities such as raisins, grapes, and wine.     

Characterisation of anthocyanins in red cabbage using high resolution liquid chromatography coupled with photodiode array detection and electrospray ionization-linear ion trap mass spectrometry

The aim of this work was to analyse and tentatively identify anthocyanin species in red cabbage using HPLC/DAD-ESI/Qtrap MS. The extraction was realized by using a pressurized liquid technique and the separation of the pigments was achieved by a high resolution liquid chromatography system with a 1.8 μm particles C-18 column. Photodiode array detection was employed to determine the UV/Vis spectral characteristic of the pigments. Electrospray ionization-linear ion trap mass spectrometry allowed the specific determination of the fragmentation patterns of the anthocyanins, by performing different ion scan modes. Twenty four anthocyanins were separated and identified, all having cyanidin as aglycon, represented as mono- and/or di-glycoside, and acylated, or not, with aromatic and aliphatic acids. Nine anthocyanins were identified for the first time in red cabbage.     

Dielectric properties of porous silicon for use as a substrate for the on-chip integration of millimeter-wave devices in the frequency range 140 to 210?GHz

In this work, the dielectric properties of porous Si for its use as a local substrate material for the integration on the Si wafer of millimeter-wave devices were investigated in the frequency range 140 to 210 GHz. Broadband electrical characterization of coplanar waveguide transmission lines (CPW TLines), formed on the porous Si layer, was used in this respect. It was shown that the dielectric parameters of porous Si (dielectric permittivity and loss tangent) in the above frequency range have values similar to those obtained at lower frequencies (1 to 40 GHz). More specifically, for the samples used, the obtained values were approximately 3.12 ± 0.05 and 0.023 ± 0.005, respectively. Finally, a comparison was made between the performance of the CPW TLines on a 150-μm-thick porous Si layer and on three other radiofrequency (RF) substrates, namely, on trap-rich high-resistivity Si (trap-rich HR Si), on a standard complementary metal-oxide-semiconductor (CMOS) Si wafer (p-type, resistivity 1 to 10 Ω.cm) and on quartz.

PACS

84.40.-x; 77.22.Ch; 81.05.Rm     

On Supporting Secure Information Distribution in Heterogeneous Systems Using Standard Technologies

This paper presents an integrated security architecture for heterogeneous distributed systems. Based on the MPEG-21 standard data structures and the MPEG-M standard services, the proposed architecture provides a unified, fine-grained solution for protecting each information unit circulated in the system. In this context, a novel scheme for translating the access control rules, initially expressed by means of the standard MPEG-21 Rights Expression Language, into Ciphertext-Policy Attribute-Based Encryption access trees is introduced, thereby enabling offline authorization based on the users’ attributes, also encapsulated and certified using MPEG-21 licenses. The proposed framework provides a detailed approach in all the steps of the information protection process, from attribute acquisition to data encryption and decryption.     

Component sizing for an autonomous wind-driven desalination plant

Objective of this paper is to provide insight in the component selection criteria of an autonomous wind-driven desalination plant. For this purpose, a suitable logistic model of such a system is developed, which simulates its steady-state operation, taking into account the power and energy equilibrium in the system. The simulation of the system operation is performed employing two alternative control strategies and a variety of different configurations with respect to the size of its main components (wind turbine, desalination plant and batteries). For each case, the annual water production is calculated and an economic assessment is performed to estimate the expected water production cost, which is the ultimate measure of the feasibility of the stand-alone system. Other important factors, such as the desalination unit start/stop operations are also calculated. Based on the simulation results, conclusions are drawn regarding the optimal sizing of the system components and its recommended operating strategy.