Structural Study of the Partially Disordered Full‐Length δ Subunit of RNA Polymerase from Bacillus subtilis

The partially disordered δ subunit of RNA polymerase was studied by various NMR techniques. The structure of the well‐folded N‐terminal domain was determined based on inter‐proton distances in NOESY spectra. The obtained structural model was compared to the previously determined structure of a truncated construct (lacking the C‐terminal domain). Only marginal differences were identified, thus indicating that the first structural model was not significantly compromised by the absence of the C‐terminal domain. Various ¹⁵N relaxation experiments were employed to describe the flexibility of both domains. The relaxation data revealed that the C‐terminal domain is more flexible, but its flexibility is not uniform. By using paramagnetic labels, transient contacts of the C‐terminal tail with the N‐terminal domain and with itself were identified. A propensity of the C‐terminal domain to form β‐type structures was obtained by chemical shift analysis. Comparison with the paramagnetic relaxation enhancement indicated a well‐balanced interplay of repulsive and attractive electrostatic interactions governing the conformational behavior of the C‐terminal domain. The results showed that the δ subunit consists of a well‐ordered N‐terminal domain and a flexible C‐terminal domain that exhibits a complex hierarchy of partial ordering.     

Design and performance analysis of a nanoscaled inverter based on wrap-aroundgate nanowire MOSFETs

The design and analysis of a silicon nanowire inverter with a wrap-around-gate nMOS is presented and its performance is compared with that of a conventional inverter. The analysis shows that the nano-channel structure design can improve carrier mobility by suppressing the transverse component of the electric field. This results in an enhancement in the current drive of the nMOS, and contributes to lowering power consumption and the switching delay. Simulated power consumption and rise time of the proposed design was found to be about 20 μW and 0.5 ns, respectively, compared with 2.5 mW and 1.5 ns achievable with conventional planar MOSFETs. Investigation of the gate length shows that a nMOS with shorter gates have an improved switching response compared with long channel devices.     

Partition coefficients of environmentally important phenols in a supercritical carbon dioxide-water system from cocurrent extraction without analysis of the compressible phase

Partition coefficients of phenol, salicylic acid, and several environmentally important chloro- and nitrophenols in a supercritical CO2-water system were measured using direct cocurrent extraction of aqueous solutions of the individual solutes with CO2. Partitioning data on the nitrophenols and salicylic acid were obtained for the first time. To bypass the troublesome and error-prone analysis of the CO2-rich phase, the present method employed only the solute concentrations in the aqueous phase before and after extraction to determine the partition coefficient. Unlike most previous engineering studies of phenol partitioning in a CO2-water system, the concentrations of phenolic solutes approached infinite dilution in both phases. This makes the results relevant to analytical-scale SFE of environmental water samples with CO2. Because of effective infinite dilution of the solutes, the partition coefficients provide a direct measure of relative CO2-philicity/hydrophilicity of the individual phenols. Compared to the octanol-water partition coefficients of substituted phenols, the CO2-water partition coefficients are more sensitive to substitution in the position neighboring the hydroxyl group.     

Electrochemical recognition of chiral species using quaternary ammonium binaphthyl salts

The use of ephedrine-substituted quaternary ammonium binaphthyl salts as molecular receptors is demonstrated. The electrochemical oxidation of the receptor is affected by the binding of an analyte in solution. The binding site on the binaphthyl salt has been determined using computer modeling and confirmed using 1D and 2D NMR studies. It is shown that the sensitivity of the receptor is related to the size of the analyte. Axially chiral binaphthyl salts are shown to bind chiral analytes in a different manner and this is demonstrated using lactic and mandelic acid. The presence of a polar functional group on the analyte is also shown to have an effect on the guest-host interaction.     

Systemic classification of concern-based design methods in the context of enterprise architecture

Enterprise Architecture (EA) is a relatively new domain that is rapidly developing. “The primary reason for developing EA is to support business by providing the fundamental technology and process structure for an IT strategy” [TOGAF]. EA models have to model enterprises facets that span from marketing to IT. As a result, EA models tend to become large. Large EA models create a problem for model management. Concern-based design methods (CBDMs) aim to solve this problem by considering EA models as a composition of smaller, manageable parts—concerns. There are dozens of different CBDMs that can be used in the context of EA: from very generic methods to specific methods for business modeling or IT implementations. This variety of methods can cause two problems for those who develop and use innovative CBDMs in the field of Enterprise Architecture (EA). The first problem is to choose specific CBDMs that can be used in a given EA methodology: this is a problem for researchers who develop their own EA methodology. The second problem is to find similar methods (with the same problem domain or with similar frameworks) in order to make a comparative analysis with these methods: this is a problem of researchers who develop their own CBDMs related to a specific problem domain in EA (such as business process modeling or aspect oriented programming). We aim to address both of these problems by means of a definition of generic Requirements for CBDMs based on the system inquiry. We use these requirements to classify twenty CBDMs in the context of EA. We conclude with a short discussion about trends that we have observed in the field of concern-based design and modeling.     

COCOVILA – Compiler-Compiler for Visual Languages

A compiler-compiler for visual languages is presented. It has been designed as a framework for building visual programming environments that translate schemas into textual representation as well as into programs representing the deep meaning of schemas. The deep semantics is implemented by applying attribute grammars to schema languages; attribute dependencies are implemented as methods of Java classes. Unlike compiler-compilers of textual languages, a large part of the framework is needed for support of interactive usage of a visual language.     

Improved pump-beam distribution in a diode side-pumped solid-state laser with a highly diffuse, cross-axis beam delivery system

A new cavity for diode side-pumped solid-state lasers was designed, built, and tested. The results were efficient absorption of pumped light in the media and homogeneous pumped-beam distribution under various pump-power levels, which generated Nd:YAG active media of different radii and concentrations and shifts of the diode wavelength. Full-multimode quasi-continuous pump operation with a slope efficiency of 40.5% was obtained from a plane-plane resonator equipped with two Nd:YAG rods. At a maximum average pump power of 1.33 kW, the optical-to-optical efficiency was 31.4%. Single-transverse-mode operation (M(2) factor of less than 1.1) of 163 W was demonstrated.     

Poly(methyl methacrylate) and polyacrylonitrile dispersions stabilized by gelatin

Methyl methacrylate was polymerized in an aqueous medium in the presence of gelatin using potassium persulfate as initiator. The dispersion mode of polymerization, when the monomer is completely miscible with water, was investigated and compared with an emulsion process, which proceeds at higher monomer concentration. Spherical and relatively uniform polymer particles were formed. Macroscopic precipitation of polymer is prevented by combination of the steric stabilization by grafted gelatin and of repulsive electrostatic interactions from the initiator residues attached to the particle surface. Static and dynamic light scattering have been used to determine the molar mass (molar mass of the whole dispersion particle, M_wD ～ 10⁸-10⁹ g mol^?1) and hydrodynamic radius (R_hD ～ 50-120 nm) of the particles. The number of particles per unit volume does not depend on overall monomer concentration, and it is higher, and therefore the particle size is smaller, than that observed for the soapless emulsion polymerization. The addition of gelatin may be thus used to modify the particle size. Acrylonitrile dispersions were prepared under similar conditions. Unlike methyl methacrylate, this monomer does not swell the polymer particles. While poly(methyl methacrylate) particles are spherical and relatively uniform, the polyacrylonitrile dispersions consist of polydisperse aggregates of tiny polymer particles.