Studies of the neutralizing activity and avidity of anti-human immunodeficiency virus type 1 Env antibody elicited by DNA priming and protein boosting

DNA vaccination is an effective means of eliciting strong antibody responses to a number of viral antigens. However, DNA immunization alone has not generated persistent, high-titer antibody and neutralizing antibody responses to human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env). We have previously reported that DNA-primed anti-Env antibody responses can be augmented by boosting with Env-expressing recombinant vaccinia viruses. We report here that recombinant Env protein provides a more effective boost of DNA-initiated antibody responses. In rabbits primed with Env-expressing plasmids, protein boosting increased titer, persistence, neutralizing activity, and avidity of anti-Env responses. While titers increased rapidly after boosting, avidity and neutralizing activity matured more slowly over a 6-month period following protein boosting. DNA priming and protein immunization with HIV-1 HXB-2 Env elicited neutralizing antibody for T cell line-adapted, but not primary isolate, viruses. The most effective neutralizing antibody responses were observed after priming with plasmids which expressed noninfectious virus-like particles. In contrast to immunizations with HIV-1 Env, DNA immunizations with the influenza virus hemagglutinin glycoprotein did not require a protein boost to achieve high-titer antibody with good avidity and persistence.     

对梁,底组合步进式加热炉半热滑轨的改进实践

介绍了间断式半热滑轨的研制和应用过程，以及经过节能技术改造，用新型半热滑轨代替老式滑轨的使用。实践表明，不但解决了以往的水梁漏水的问题，而且使线Ｃ级品率提高了３．３９％，每年可增产３５００ｔ线材，吨钢热涌单耗降低０．１４ＧＪ，年创经济效益２３９万元。     

不同光照下梅尼小环藻对昼夜温差变化的生理响应

为探讨硅藻在不同环境中对昼夜温差变化的响应机制,以梅尼小环藻为代表,研究了在高光照与低光照两种条件下,3种不同昼夜温差对梅尼小环藻生物量、光合活性、营养盐吸收利用的影响。试验结果表明:在5~10 ℃范围内,昼夜温差的减小能促进梅尼小环藻细胞密度与叶绿素a的增长,使其比生长速率增加;在高光照下,各组梅尼小环藻的最大量子产量(F_v/F_m)差异显著,降幅范围为36.3%~65.2%(P<0.05),而低光照下各组F_v/F_m的组间差异不显著(P>0.05);此外,随着温差减小,培养基中PO^3-₄-P的净减小值越大;不同处理组梅尼小环藻对硅的亲和力也表现出差异:高光照下,5 ℃温差组的硅亲和力最高,其半饱和常数(K_m)为1.755 ng/L,而低光照下,恒温组的亲和力最高,其K_m为4.673 ng/L;不同光照条件下,梅尼小环藻的生理习性对昼夜温差的响应不同,但总体上一定范围内昼夜温差减小有利于梅尼小环藻的生长繁殖。     

Rate-smoothed schedule with tolerable data dropping for video coding stream

Most algorithms of smoothing schedule compute the required bit rate of video transmission to satisfy all the transmitted data. In this paper, our proposed tolerable data dropping algorithm can adjust transmitting data to fit available bit rate. MPEG-4 with fine grained scalability (FGS) can support partial data dropping to adapt to available bandwidth network. The algorithm is based on the minimum variance bandwidth allocation (MVBA) algorithm proposed by Salehi et al. to compute the bit rate such that still ensuring that the buffer never underflows and overflows for MPEG-4 FGS streams under the limited bandwidth resource. We prove that our proposed algorithm, named MVBADP, is smoother than the MVBA algorithm. The experimental results show the peak rate, the number of rate changes, and the ratio of total dropping data, and the PSNR for four test sequences with different content characteristics. They are varied by buffer sizes and tolerable dropping ratios. We found that the MVBADP algorithm can reduce the peak rate and the number of changes when the transmitted data are dropped by tolerable dropping ratio, especially on the video sequences with the high motion and complex texture characteristic and larger size change of the consecutive frame.     

“绕射积分偏移”的成象原理及提高其偏移剖面质量的探讨

“绕射积分偏移”的过程一直都是用绕射波收敛理论来描述的.但是这个理论不能解释为什么经过“绕射积分偏移”的剖面会有信噪比降低和弧形干扰的现象.在这时,我们用一个简单的数学模型,并理想化了一些条件,弄清了“绕射积分偏移”成像的真正原理.我们发现在任一CDP点下面的反射界面点的位置应与某一双曲线的顶点时间相当,这个双曲线正好处在与相应的反射t_0剖面上各道波至所形成的直线相切的位置上.在此结果的基础上,本文提出了减少干扰和改善剖面质量的有效措施.我们希望它能尽早为实际工作所采用.另外,本文也对目前流行的几种类型的所谓“克希霍夫积分偏移”进行评述并指出它们的缺点.     

N3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling

There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N₃-kethoxal (N₃K)-based bioorthogonal intracellular RNA functionalization. With N₃K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells.     

单,双向拉伸各性能指标间的相关性研究

采用９种板料进行了单拉及液压胀形（双向拉伸）试验，分别研究了双向拉伸各性能指标间及单、双向伸各性能指标间的相关关系，得到了一系列有意义的结果。     

溶剂抽提偏三甲苯-正辛烷三元体系液-液相平衡的对比分析

在70℃下采用浊点法分别测定了二甲亚砜、N-甲酰吗啉、四甘醇与偏三甲苯-正辛烷三元体系液-液相平衡数据，得到体系的液-液分层曲线和结点数据，并用液-液分层曲线和体系物性（折光指数、密度）相结合的方法对平衡数据进行了分析。结果表明：溶剂的溶解能力的次序为：N-甲酰吗啉＞二甲驱砜＞四甘醇；溶剂的选择性次序为：二甲亚砜＞四甘醇＞N-甲酰吗啉，三种溶剂中二甲亚砜同时具有较高的溶解能力和选择性，较适合萃取偏三甲苯-正辛烷的混合溶液。研究结果可以为溶剂抽提过程相平衡数据库提供基础数据，并为重芳烃抽提的溶剂选择提供参考。     

高效液相色谱法测定止痛养胃软胶囊中延胡索乙素的含量

测定止痛胃软胶囊中延索乙素的含量,可用高效液相色谱法。选用甲醇—磷酸缓冲溶液(40∶60)为流动相,测定波长280nm。结果:延胡索乙素浓度在0.02140～1.37mg/mL范围内,与峰面积线性关系良好,y=(8.390×106)x+7.505×103(r=0.9997)。结论:该法操作简便、快捷、特异性强,可用于控制止痛养胃软胶囊的质量。