Dietary linoleic acid increases and palmitic acid decreases hepatic LDL receptor protein and mRNA abundance in young pigs

The present study was conducted to determine the effects of dietary fatty acids on hepatic LDL receptor (LDLr) protein abundance and mRNA levels. Sixty pigs were randomized into 10 groups and fed corn-soybean meal diets containing three cholesterol levels (0.25%, 0.5%, and 1.0%, w/w) with no added fat, or fats rich (30% of calories) in palmitic acid or linoleic acid. A control group was fed the base diet with no added fat. After 30 days, plasma LDL-cholesterol (LDL-C) levels increased as the dietary cholesterol increased (P < 0.05); however, there was no significant effect of either fatty acid. Dietary fatty acids, however, had distinctly different effects on hepatic LDLr protein (analyzed by ELISA) and mRNA (analyzed by Northern blot) abundance. When pigs consumed diets containing 0.25% cholesterol, linoleic acid increased hepatic LDLr protein 40% whereas palmitic acid reduced it 40% (P < 0.05). These changes in LDLr protein abundance were accompanied by parallel changes in hepatic LDLr mRNA; linoleic acid increased LDLr mRNA 2-fold (P < 0.01), whereas palmitic acid decreased it 60% (P < 0.01). The differential effects of fatty acids on LDLr expression were only observed at 0.25% cholesterol, suggesting that higher intakes of cholesterol have a dominant and repressive effect on regulation of LDLr expression. Cholesterol intake increased hepatic total cholesterol levels (P < 0.01) while dietary fatty acids had no effect on hepatic sterols. In summary, our results indicate that dietary linoleic acid and palmitic acid have markedly different effects on hepatic LDLr protein abundance that are mediated by differential effects on LDLr mRNA and protein levels. Further studies are needed to fully elucidate the molecular mechanisms by which fatty acids regulate LDLr mRNA and protein levels.     

Use of tilmicosin in a severe outbreak of respiratory disease in weaned beef calves

In a liability lawsuit an expertise had to answer the question whether a mania in the course of an affective psychosis could have been caused by chronic mercury intoxication resulting from dental amalgam fillings. On the basis of current psychiatric and toxicological knowledge, such an association can be disproved. Mercury intake from amalgam fillings does not lead to toxic concentrations in organs or body fluids. Therefore physicians and dentists should avoid alarming patients and thus causing iatrogenic harm.     

Visualizing diversity and depth over a set of objects.

In many domains, the user is interested not only in including objects with particular desired values, but also in the distribution of values in the the set. Our approach for visualizing a set of objects uses glyphs overlaid on a composite representation of the entire set to convey objects' depth and the set's diversity. We test and apply this technique to three application domains: analyzing student applicant pools of a particular school or department, building an effective fantasy football team, and analyzing traffic activity on a network.     

Degradable networks formed from multi-functional poly(vinyl alcohol) macromers: comparison of results from a generalized bulk-degradation model for polymer networks and experimental data

Experimental and theoretical approaches were used to understand the degradation behavior of networks formed from the chain polymerization of multi-functional macromers. A typical experimental mass loss profile and theoretical model are presented and discussed to illustrate the various regions of erosion. Specifically, the degree of connectivity of the network was shown to influence the percent mass loss at reverse gelation, and the percent cyclization was shown to influence the overall shape of the mass loss profile. Finally, the theoretical predictions of mass loss were compared to experimental data for macromers with variations in their molecular weight and degree of functionality (i.e. variations in the initial network crosslinking density). Changes in the volumetric swelling ratio and compressive modulus were measured with time to obtain the degradation kinetic constant for each macromer system, ranging from 1.5×10⁻⁴ min⁻¹ to 3.0×10⁻⁵ min⁻¹. Results demonstrate that the theoretical predictions capture the mass loss profiles of all three systems, and as the crosslinking density of the initial network was decreased the overall time for complete degradation of the gels was also decreased.     

High resolution SE-fMRI in humans at 3 and 7 T using a motor task

Object The sensitivity of spin echo (SE) experiments to blood oxygenation level dependent (BOLD) contrast was explored in a study of the same six subjects carried out at 3 and 7 T. Materials and methods Multi-slice, single shot, spin echo, echo planar images with a voxel size of 1 × 1 × 3 mm³ were acquired at three different echo times, during execution of a simple motor task. Results Significant activation was observed at all echo times at both field strengths. Analysis of the fractional signal change as a function of echo time indicated that the change in relaxation rate, ΔR ₂, at 7 T was −0.51 ± 0.14 s ⁻¹, which was 1.3 times larger than the value found at 3 T. Measurements of the percentage signal change on activation and temporal signal to noise ratio showed that there was an increase in the BOLD contrast to noise ratio (CNR) at 7 versus 3 T by a factor of 1.9. There was no overlap of areas of significant activation in the SE data acquired at either field strength with the site of large veins. Conclusion SE-BOLD CNR in motor cortex was found to increase significantly at 7 T compared with 3 T.     

Art and robotics: sixty years of situated machines

This paper pursues the intertwined tracks of robotics and art since the mid 20th century, taking a loose chronological approach that considers both the devices themselves and their discursive contexts. Relevant research has occurred in a variety of cultural locations, often outside of or prior to formalized robotics contexts. Research was even conducted under the aegis of art or cultural practices where robotics has been pursued for other than instrumental purposes. In hindsight, some of that work seems remarkably prescient of contemporary trends. The context of cultural robotics is a highly charged interdisciplinary test environment in which the theory and pragmatics of technical research confronts the phenomenological realities of physical and social being in the world, and the performative and processual practices of the arts. In this context, issues of embodiment, material instantiation, structural coupling, and machine sensing have provoked the reconsideration of notions of (machine) intelligence and cognitivist paradigms. The paradoxical condition of robotics vis-à-vis artificial intelligence is reflected upon. This paper discusses the possibility of a new embodied ontology of robotics that draws upon both cybernetics and post-cognitive approaches.