The Approximation and Computation of a Basis of the Trace Space <Emphasis Type="Italic">H</Emphasis><Superscript>1/2</Superscript>

We present a method for construction of an approximate basis of the trace space H ^1/2 based on a combination of the Steklov spectral method and a finite element approximation. Specifically, we approximate the Steklov eigenfunctions with respect to a particular finite element basis. Then solutions of elliptic boundary value problems with Dirichlet boundary conditions can be efficiently and accurately expanded in the discrete Steklov basis. We provide a reformulation of the discrete Steklov eigenproblem as a generalized eigenproblem that we solve by the implicitly restarted Arnoldi method of ARPACK. We include examples highlighting the computational properties of the proposed method for the solution of elliptic problems on bounded domains using both a conforming bilinear finite element and a non-conforming harmonic finite element. In addition, we document the efficiency of the proposed method by solving a Dirichlet problem for the Laplace equation on a densely perforated domain.     

Distributed and collaborative visualization of large data sets using high-speed networks

We describe an architecture for distributed collaborative visualization that integrates video conferencing, distributed data management and grid technologies as well as tangible interaction devices for visualization. High-speed, low-latency optical networks support high-quality collaborative interaction and remote visualization of large data.     

High-definition multimedia for multiparty low-latency interactive communication

We describe a high-quality collaborative environment that uses High-Definition (HD) video to achieve near realistic perception of a remote site. The capture part, consisting of a HD camera, Centaurus HD-SDI capture card, and UltraGrid software, produces a 1.5 Gbps UDP data stream of uncompressed HD video that is transferred over a 10GE network interface to the high-speed IP network. The HD video stream displaying uses either a software-based solution with color depth down-sampling and field de-interlacing, or another Centaurus card. Data distribution to individual participants of the videoconference is achieved using a user-controlled UDP packet reflector based on the Active Element idea. The viability of this system has been demonstrated at the iGrid 2005 conference for a three-way high quality videoconference among sites in the Czech Republic, Louisiana, and California.     

Optimal signal estimation in neuronal models

We study optimal estimation of a signal in parametric neuronal models on the basis of interspike interval data. Fisher information is the inverse asymptotic variance of the best estimator. Its dependence on the parameter value indicates accuracy of estimation. Our models assume that the input signal is estimated from neuronal output interspike interval data where the frequency transfer function is sigmoidal. If the coefficient of variation of the interspike interval is constant with respect to the signal, the Fisher information is unimodal, and its maximum for the most estimable signal can be found. We obtain a general result and compare the signal producing maximal Fisher information with the inflection point of the sigmoidal transfer function in several basic neuronal models.     

Unambiguous assignment of intramolecular chemical cross-links in modified mammalian membrane proteins by Fourier transform-tandem mass spectrometry

Fourier transform tandem mass spectrometry (FT-MS/MS) can be used to unambiguously assign intramolecular chemical cross-links to specific amino acid residues even when two or more possible cross-linking sites are adjacent in the cross-linked protein. Bovine rhodopsin (Rho) in its dark-adapted state was intramolecularly cross-linked with lysine-cysteine (K-C) or lysine-lysine (K-K) cross-linkers to obtain interatomic distance information. Large, multiply charged, cross-linked peptide ions containing adjacent lysines, corresponding to Rho(50-86) (K(66) or K(67)) cross-linked to Rho(310-317) (C(316)) or Rho(318-348) (K(325) or K(339)), were fragmented by collision-induced dissociation (CID), infrared multiphoton dissociation (IRMPD), and electron capture dissociation (ECD). Complementary sequence-specific information was obtained by combining cross-link assignments; however, only ECD revealed full palmitoylation of adjacent cysteines (C(322) and C(323)) and cross-linking of K(67) (and not K(66)) to C(316), K(325), and K(339). ECD spectra contained crucial c- and z-ions resulting from cleavage of the bond between K(66) and K(67). To our knowledge, this work also presents the first demonstration that ECD can be used to characterize S-linked fatty acid acylation on cysteines. The comprehensive fragmentation of large peptides by CID, IRMPD, and particularly ECD, in conjunction with the high resolution and mass accuracy of FT-MS/MS, is shown to be a valuable means of characterizing mammalian membrane proteins with both chemical and posttranslational modifications.     

Study of the branched hydrocarbon fraction of intramuscular lipids from Iberian dry-cured ham

Branched hydrocarbons from the unsaponifiable lipid fraction of intramuscular fat from Iberian dry-cured hams were analyzed. Thirty-four hams were distributed into four groups according to the feed system of the pigs (extensive system "Montanera" and intensive system "Pienso") and genotype (Iberian and Iberian×Duroc). A total of 35 different hydrocarbons were identified in this study. Some differences were found in the levels of branched, cyclical and unsaturated hydrocarbons as a function of the diet of the pigs (3-hexyl-1,1-dimethyl cyclopentane, p<0.05; 3-octadecene, p<0.01; neophytadiene, p<0.001; 1-ethylundecyl benzene, p<0.01; cyclohexadecane, p<0.001; cyclotriacontane, p<0.05). However, the genotype had a limited effect in the level of branched hydrocarbons found. The study of branched hydrocarbons in intramuscular fat from dry-cured Iberian ham allows differentiation between the pigs management systems.     

RETRACTED ARTICLE: The influence of surface cleaning on the stability of Pd/GaAs contacts

The thermal stability of –GaAs ohmic contacts with Ge and Sn layers was investigated at 300 and 400 °C. The majority of contact structures are possible to anneal according to the annealing optimization at one temperature, but the dependence of the contact resistivity on the annealing temperature show two minima in the case of the Ge(20 nm)/Pd(10 nm) structure. The thermal stability of the structure is better after the annealing at temperatures from the higher temperature minimum. Etching of GaAs wafers before the metal deposition in the solution of (1 : 8 : 500) followed by (1 : 1) or in concentrate HCl produces the best thermal stability. The Ge/Pd contact structures are based on the solid phase regrowth mechanisms but the annealing mechanism is completely different for the Sn/Pd contact structures.     

Micromachined nanocalorimetric sensor for ultra-low-volume cell-based assays

Current strategies for cell-based screening generally focus on the development of highly specific assays, which require an understanding of the nature of the signaling molecules and cellular pathways involved. In contrast, changes in temperature of cells provides a measure of altered cellular metabolism that is not stimulus specific and hence could have widespread applications in cell-based screening of receptor agonists and antagonists, as well as in the assessment of toxicity of new lead compounds. Consequently, we have developed a micromachined nanocalorimetric biological sensor using a small number of isolated living cells integrated within a subnanoliter format, which is capable of detecting 13 nW of generated power from the cells, upon exposure to a chemical or pharmaceutical stimulus. The sensor comprises a 10-junction gold and nickel thermopile, integrated on a silicon chip which was back-etched to span a 800-nm-thick membrane of silicon nitride. The thin-film membrane, which supported the sensing junctions of the thermoelectric transducer, gave the system a temperature resolution of 0.125 mK, a low heat capacity of 1.2 nJ mK(-1), and a rapid (unfiltered) response time of 12 ms. The application of the system in ultra-low-volume cell-based assays could provide a rapid endogenous screen. It offers important additional advantages over existing methods in that it is generic in nature, it does not require the use of recombinant cell lines or of detailed assay development, and finally, it can enable the use of primary cell lines or tissue biopsies.     

Mechanism of Primary Purification in Zone Melting of Boron-Containing Powder Materials

We have established the regular behavior of the change in chemical composition and grain size of refractory material when sintered in the presence of a moving liquid phase under conditions including a high temperature gradient and continuous heating, and on this basis we propose a mechanism for primary purification to remove impurities from boron-containing powder materials during zone melting. We have shown that the degree of purification depends on the completeness of recrystallization of particles of the refractory material through the solvent melt, and is determined by the physical and processing properties of the original powders, the temperature and time parameters for the zone melting, the structure of the phase diagram, and the kinetics of diffusional interaction processes in the system refractory material solvent for the impurities.     

Evolution of volatile aldehydes in Iberian ham matured under different processing conditions

To evaluate the influence of the Iberian ham processing conditions in the evolution of volatile aldehydes, 35 hams were processed in two plants following different conditions of relative humidity and temperature. For this, free fatty acids, peroxide values and volatile aldehydes were quantified in the hams. The highest increases in free fatty acids were noted during the drying stage in both processing plants. The drying period also revealed the greatest increase in peroxide values, where the highest values were in those hams processed at higher temperatures. The temperature during post-salting and drying had a marked influence on the formation of volatile aldehydes, being responsible for the differences in volatile compounds of matured hams.