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101.
102.
Scialfa Charles T.; Hamaluk Eleanor; Skaloud Petra; Pratt Jay 《Canadian Metallurgical Quarterly》1999,14(4):695
Younger and older adults were asked to saccade to an orientation-defined target that was presented alone, with a more central distractor, or with a more peripheral distractor. Both age groups exhibited saccadic averaging that was more pronounced in the central distractor condition, wherein older adults had the larger effect. These results are relevant to questions of oculomotor control and also have implications for the study of age differences in other visually guided behaviors. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
103.
Zusammenfassung Das Zeichnen von Graphen ist ein junges aufblühendes Gebiet der Informatik. Es befa?t sich mit Entwurf, Analyse, Implementierung
und Evaluierung von neuen Algorithmen für ?sthetisch sch?ne Zeichnungen von Graphen. Anhand von selektierten Anwendungsbeispielen,
Problemstellungen und L?sungsans?tzen wollen wir in dieses noch relativ unbekannte Gebiet einführen und gleichzeitig einen
überblick über die Aktivit?ten und Ziele einer von der DFG im Rahmen des Schwerpunktprogramms „Effiziente Algorithmen für
Diskrete Probleme und ihre Anwendungen“ gef?rderten Arbeitsgruppe aus Mitgliedern der Universit?ten Halle, K?ln und Passau
und des Max-Planck-Instituts für Informatik in Saarbrücken geben.
Eingegangen am 22.05.1996, in überarbeiteter Form am 19.02.1997 相似文献
104.
In order to increase the wear resistance of metal forming tools, four different cold and hot working steels were laser-beam surface melted and alloyed with titanium carbide and vanadium carbide. The layers were investigated concerning chemical composition, microstructure, hardness profiles, wear behaviour measured by pin-on-disk-tests with different counterbodies and plastic behaviour of the surface by a new developed repeated impact testing machine. It turned out to be more useful to take a substrate which is already quenched and tempered prior to the laser-beam surface treatment than to carry out the tool hardening heat treatment afterwards. A tempering step after the laser-beam alloying offers the advantage of carbide precipitates in the layer connected with an increase in the hardness and a reduction of residual stresses. By means of laser-beam surface alloying the wear protection of the layer can be increased while the alloying content of the substrate is reduced with respect to the core fracture toughness. The optimal alloying addition depends on the demands of the envisaged application. 相似文献
105.
Petra Dassen-Housen 《AI & Society》2002,16(3):188-209
In this paper, some fundamental aspects of societal change processes are described, leading to proposals of how to cope with
such changes through continuous learning within society. This change society is presently emerging worldwide. It is very much
shaped by advanced networked information and communication technology. Correspondingly, certain trends are identified in this
paper which indicate the change processes towards this new emerging society. Subsequently, different personal skills are described
which are required for all members of society to cope with these trends. The paper finishes with a case study which deals
with regional development through open networked business processes. The aim of the project described was to develop a joint
family vacation concept in the former socialist East German state of Saxonia. Thus, it illustrates the change and learning
processes across society which have taken place in many countries after the socialist era. 相似文献
106.
107.
Derivatization of Antibody Fab Fragments: A Designer Enzyme for Native Protein Modification 下载免费PDF全文
Dr. Sandra Liebscher Dr. Petra Kornberger Gerhard Fink Dr. Eva‐Maria Trost‐Gross Dr. Eva Höss Prof. Dr. Arne Skerra Prof. Dr. Frank Bordusa 《Chembiochem : a European journal of chemical biology》2014,15(8):1096-1100
Bioconjugates, such as antibody–drug conjugates, have gained recent attention because of their increasing use in therapeutic and diagnostic applications. Commonly used conjugation reactions based upon chemoselective reagents exhibit a number of drawbacks: most of these reactions lack regio‐ and stereospecificity, thus resulting in loss of protein functionality due to random modifications. Enzymes provide an obvious solution to this problem, but the intrinsic (natural) substrate specificities of existing enzymes pose severe limitations to the kind of modifications that can be introduced. Here we describe the application of the novel trypsin variant trypsiligase for site‐specific modification of the C terminus of a Fab antibody fragment via a stable peptide bond. The suitability of this designed biocatalyst was demonstrated by coupling the Her2‐specific Fab to artificial functionalities of either therapeutic (PEG) or diagnostic (fluorescein) relevance. In both cases we obtained homogeneously modified Fab products bearing the artificial functionality exclusively at the desired position. 相似文献
108.
Hedderich R Müller R Greulich Y Bannert N Holland G Kaiser P Reissbrodt R 《International journal of food microbiology》2011,146(1):105-107
Colony counting by spreading bacterial suspensions on plating media by various techniques is of general concern. Comparative studies between hand plating (Drigalski-spatula technique) for different time intervals and spiral plating resulted in significant differences in colony counts. Lower counts of Gram-negative bacteria were obtained by using hand plating for more than 10 s, compared with short time hand plating (5 s) or spiral plating. Colony counting of Gram-positive bacteria showed no differences between both techniques. Further characterisation of Escherichia coli cells spread with the Drigalski-spatula technique by electron microscopy revealed a large number of damaged cells compared to control samples. The data clearly shows that the mechanical forces during hand plating are sufficient to damage E. coli cells. 相似文献
109.
Accurate spectral calibration of airborne and spaceborne imaging spectrometers is essential for proper preprocessing and scientific exploitation of high spectral resolution measurements of the land and atmosphere. A systematic performance assessment of onboard and scene-based methods for in-flight monitoring of instrument spectral calibration is presented for the first time in this paper. Onboard and ground imaging data were collected at several flight altitudes using the Airborne Prism Experiment (APEX) imaging spectrometer. APEX is equipped with an in-flight characterization (IFC) facility allowing the evaluation of radiometric, spectral, and geometric system properties, both in-flight and on-ground for the full field of view. Atmospheric and onboard filter spectral features present in at-sensor radiances are compared with the same features in reference transmittances convolved to varying instrument spectral configurations. A spectrum-matching algorithm, taking advantage of the high sensitivity of measurements around sharp spectral features toward spectrometer spectral performance, is used to retrieve channel center wavelength and bandwidth parameters. Results showed good agreement between spectral parameters estimated using onboard IFC and ground imaging data. The average difference between estimates obtained using the O(2) and H(2)O features and those obtained using the corresponding filter features amounted to about 0.3 nm (0.05 of a spectral pixel). A deviation from the nominal laboratory instrument spectral calibration and an altitude-dependent performance was additionally identified. The relatively good agreement between estimates obtained by the two approaches in similar spectral windows suggests they can be used in a complementary fashion: while the method relying on atmospheric features can be applied without the need for dedicated calibration acquisitions, the IFC allows assessment at user-selectable wavelength positions by custom filters as well as for the system on-ground. 相似文献
110.
Walter A Schumacher W Bocklitz T Reinicke M Rösch P Kothe E Popp J 《Applied spectroscopy》2011,65(10):1116-1125
Classification of Raman spectra recorded from single cells is commonly applied to bacteria that exhibit small sizes of approximately 1 to 2 μm. Here, we study the possibility to adopt this classification approach to filamentous bacteria of the genus Streptomyces. The hyphae can reach extensive lengths of up to 35 μm, which can correspond to a single cell identified in light microscopy. The classification of Raman bulk spectra will be demonstrated. Here, ultraviolet resonance Raman (UV RR) spectroscopy is chosen to classify six Streptomyces species by the application of a tree-like classifier. For each knot of the hierarchical classifier, estimated classification accuracies of over 94% are accomplished. In contrast to the classification of bulk spectra, the classification of single-cell spectra requires a homogenous substance distribution within the cell. Consequently, the bacterial cell chemistry can be represented by one individual spectrum. This requirement is not fulfilled when different spectra are processed from different locations within the cell. Bacteria of the investigated genus Streptomyces exhibit, besides the normal bacterial spectra, lipid-rich spectra. The occurrence of lipid enrichment depends on culture age and nutrition availability. With this study, we investigate the cell substance distribution, especially of lipid-rich fractions. The classification utilizing a tree-like classifier is also applied to the Streptomyces single-cell spectra, resulting in classification accuracies between 80 and 93% for the investigated Streptomyces species. 相似文献