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991.
Maozhang Tian Jiaxin Zhu Jingjing Guo Xia Guo 《Journal of surfactants and detergents》2021,24(1):111-119
This study observed the activities of bromelain in the presence of various cationic surfactants at different temperatures and the conformational changes in bromelain by 1H NMR spectroscopy. We found that the bromelain activity was enhanced by tens to hundreds of micromoles per liter of the surfactant. In the presence of the surfactants, bromelain exhibited good tolerance to a range of substrate temperatures and its thermal stability was also increased. The 1H NMR experiments indicated that when the temperature was increased from 25.0 to 45.0 °C, the protons of bromelain having chemical shifts (δ) between 3.7 and 5.2 ppm moved upfield, while those having δ values between 3.2 and 3.7 ppm moved downfield. In the bromelain/cationic surfactant mixture, the values of δ for the protons in both bromelain and the cationic surfactants decreased, accompanied by the broadening of the half-peak width of the surfactant protons. These results indicated that both increasing temperature and adding a cationic surfactant made the bromelain chain more flexible and hence, increased the bromelain activity. To the best of our knowledge, this was the first time that the relationship between the protein activity and the 1H NMR data was expounded. 相似文献
992.
Asako Yamamoto Lingli Yang Yasutaka Kuroda Jiao Guo Lanting Teng Daisuke Tsuruta Ichiro Katayama 《International journal of molecular sciences》2021,22(1)
As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H2O2 in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H2O2-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo. 相似文献
993.
Operational optimization of a building-level
integrated energy system considering
additional potential benefits of energy
storage 下载免费PDF全文
Sai Liu Cheng Zhou Haomin Guo Qingxin Shi Tiancheng E. Song Ian Schomer Yu Liu 《电力系统保护与控制》2021,6(1):55-64
As a key component of an integrated energy system (IES), energy storage can effectively alleviate the problem of
the times between energy production and consumption. Exploiting the benefits of energy storage can improve the
competitiveness of multi-energy systems. This paper proposes a method for day-ahead operation optimization of a
building-level integrated energy system (BIES) considering additional potential benefits of energy storage. Based on
the characteristics of peak-shaving and valley-filling of energy storage, and further consideration of the changes in
the system’s load and real-time electricity price, a model of additional potential benefits of energy storage is
developed. Aiming at the lowest total operating cost, a bi-level optimal operational model for day-ahead operation
of BIES is developed. A case analysis of different dispatch strategies verifies that the addition of the proposed
battery scheduling strategy improves economic operation. The results demonstrate that the model can exploit
energy storage’s potential, further optimize the power output of BIES and reduce the economic cost. 相似文献
994.
针对渤海油田油井解堵效果变差、有效期降低等问题,提出了扩大解堵半径、优选解堵剂体系、强化解堵效果的解堵思路。通过工艺研究、工艺优选,形成了深穿透解堵技术。深穿透解堵技术主要包含基质解堵、降阻造缝和储层深部解堵三个过程。对深穿透解堵工艺机理进行了研究,并形成了一套现场可应用的工艺技术。针对锦州油田注聚井区堵塞问题,从聚合物溶液降黏、模拟含聚堵塞物、现场含聚堵塞物降解三个方面优选出一种复合解堵剂,结合工艺思路形成施工方案,并进行现场应用,取得了较好的增液、增油效果。 相似文献
995.
为解决设计模式挖掘正确率较低的问题, 提出一种带特征指标约束描述的设计模式分类挖掘方法。归纳了47种特征指标, 给出了设计模式约束定义, 对设计模式特征进行了描述, 以Adapter模式、Command模式、Factory Method模式为例, 分结构型、行为型与创建型3类挖掘设计模式, 设计了3个基准系统与4个经典系统的设计模式挖掘试验。试验结果表明, 本研究方法对基准系统的Adapter模式、Command模式、Factory Method模式挖掘正确率为96.13%、91.67%、72.23%, 对经典系统挖掘正确率分别为84.3%、81.26%、73.17%, 与传统方法比较, 本研究方法取得了较好的效果。 相似文献
996.
Short-term load forecasting is of great significance to the secure and efficient operation of power systems. However, loads can be affected by a variety of external impact factors and thus involve high levels of uncertainties. So it is a challenging task to achieve an accurate load forecast. This paper discusses three commonly-used machine-learning methods used for load forecasting, i.e., the support vector machine method, the random forest regression method, and the long short-term memory neural network method. The features and applications of these methods are analyzed and compared. By integrating the advantages of these methods, a fusion forecasting approach and a data preprocessing technique are proposed for improving the forecasting accuracy. A comparative study based on real load data is performed to verify that the proposed approach is capable of achieving a relatively higher forecasting accuracy. 相似文献
997.
三维空间孔道的精确校核 总被引:1,自引:0,他引:1
二维空间孔道的传统校核方法是把三维问题化成二维问题,再简化或近似处理,这样的方法在高精度场合往往不能满足设计要求;还有的是对孔表面网格化、布尔运算等,算法复杂,难于实现。本文从计算几何理论上探讨了三维空间孔的精确校核方法,简单、易行、可靠。 相似文献
998.
燃烧合成三元碳化合物Ti2AlC1-x 总被引:1,自引:0,他引:1
采用Ti,Al和C元素粉末为反应物原料,通过燃烧合成法首次成功地制备出了单相三元碳化合物Ti2AlC1-x。实验结果表明:若以“理想”晶体结构化学式Ti2AlC化学计量比为起始反应原料配比,燃烧产物主晶相为Ti2AlC2;以缺碳的非化学计量比(Ti2AlC1-x)为反应原料配比,即Ti:Al:C=3:1.5:1=2:1:0.7(摩尔比),得到单相的燃烧产物Ti2AlC1-x。从热力学原理的角度探讨了不同原料摩尔配比对燃烧产物相组成的影响机理。 相似文献
999.
以钨酸铵溶液为原料,采用超声喷雾干燥法制备了超微钨粉。研究了该方法中钨酸铵浓度对超微钨粉形貌的影响。结果表明,当溶液中钨酸铵的浓度为10%时,得到了球形度很好的非完全结晶的前驱体。当钨酸铵浓度达25%时,得到了层片状的晶态前驱体。钨酸铵溶液浓度对干燥时晶体生长速度具有重要的影响。分别采用这2种前驱体在630℃,保温30min和氢气流莆4L/min条仲下能够完全还原,得到超微α—W粉。所制得的钨粉形貌完全遗传了其前驱体的形貌,分别得到球状和层状钨粉。球状钨粉由纳米颗粒聚集而成。片层状钨粉的厚度在1mm以下。 相似文献
1000.