Immunochemical Studies on the Denaturation of Ovalbumin Stored with Glucose

The ovalbumin denaturation induced by Maillard reaction with glucose was investigated by immunochemical methods. The storage for either 2 or 10 days at 50°C and 65% relative humidity decreased the maximum immunoprecipitation to 90 and 80% of that by native protein, respectively. Ten day-stored ovalbumin was separated into two fractions with antigenicity (fr-II) and without (fr-I) by the immunoaffinity chromatography of antiovalbumin-coupled Sepharose 4B. The circular dichroism (CD) analysis showed that fr-II, containing amino groups in almost the same amount as fr-I, retained a large amount of ordered structure. Ovalbumin molecules were presumably not denatured by only the modification of amino groups with glucose in an early stage of Maillard reaction.     

REALIZATION THEORY OF DISCRETE-EVENT SYSTEMS AND ITS APPLICATION TO THE UNIQUENESS AND UNIVERSALITY OF DEVS FORMALISM

Many man-made systems have discrete event nature. Many modeling formalisms for discrete-event mechanisms have invented and been used for many problems. Among those models, the DEVS formalism is to provide natural and universal models in some sense.

This paper first provides a realization theory of general discrete-event systems. That is, a behavioral definition of discrete-event system is defined, and then a state transition function of the system is constructed. Based on the realization, the uniqueness problem of representations for discrete-event systems is positively solved. Furthermore, as an application of that solution, this paper shows both the fact that a legitimate DEVS with surjective internal transition function is unique up to isomorphism in the class of state representations of the state system defined from the DEVS, and the fact that any discrete-event system has a DEVS realization. In this sense the DEVS modeling facility has the uniqueness and universality in modeling discrete event mechanisms.     

Minimization of Reactive Power Fluctuation in JT-60SA Magnet Power Supply

This paper describes an asymmetric control method for the firing angle and a start/stop timing shift control of four thyristor converters called "Booster PS" to minimize the reactive power fluctuation during plasma initiation in JT-60SA. From the simulation using the "PSCAD/EMTDC" code, it is found that these control methods can drastically reduce the reac- tive power induced by the four units of the "Booster PS". In addition, the voltage fluctuation of the motor-generator connected to the "Booster PS" is expected to be suppressed. This can also contribute to achieve stable control of the JT-60SA magnet power supplies.     

Detailed Analysis of the Transient Voltage in a JT-60SA PF Coil Circuit

A superconducting coil system is actually complicated by the distributed parameters, e.g. the distributed mutual inductance among turns and the distributed capacitance between adjacent conductors. In this paper, such a complicated system was modeled with a reasonably simplified circuit network with lumped parameters. Then, a detailed circuit analysis was conducted to evaluate the possible voltage transient in the coil circuit. As a result, an appropriate (minimum) snubber capacitance for the Switching Network Unit, which is a fast high voltage generation circuit in JT-60SA, was obtained.     

Determination of Antigenicity by Radioimmunoassay and of Trypsin Inhibitory Activities in Heat or Enzyme Denatured Ovomucoid

The effects of heat treatment and enzymic digestion on the antigenic reactivity of ovomucoid were studied. This reactivity was mainly examined by a solid phase competitive radioimmunoassay. The trypsin inhibitory activity was also measured to elucidate its relationship to the antigenic reactivity. The antigenic reactivity and trypsin inhibitory activity diminished in parallel with increase of heating time. However, enzymic digestion caused a more rapid decrease in antigenic reactivity than in trypsin inhibitory activity. From these results, it is suggested that the structure of the antigenic determinants is destroyed in a similar manner to that of the trypsin inhibitory active site when ovomucoid is heated, while the former structure is destroyed more easily than the latter by enzymic digestion.     

Emulsifying Properties of Bovine Blood Globin: A Comparison with Some Proteins and Their Improvement

The emulsifying properties of bovine blood globin were compared with those of bovine serum albumin, hemoglobin and ovalbumin. Although the emulsifying activity of globin was greater than that of hemoglobin and ovalbumin, it decreased in the isoelectric zone owing to the low solubility of globin. Acetylation increased the emulsifying activity of globin in the isoelectric zone, but decreased in the acidic pH region. Pepsin digestion did not increase the emulsifying activity of globin, but addition of CMC to pepsin-digested globin improved both its emulsifying activity and the emulsion stability.     

结合烷烃硫醇小分子自组装单层的原子光刻技术——以壬烷硫醇和12烷硫醇为例

采用不同网格尺寸的透射电镜铜网作为掩模,以烷烃硫醇类小分子自组装单层作为光刻胶,光学质量的云母为衬底,用亚稳氦原子光刻技术制备具有纳米台阶的金膜图形,最终用原子力显微镜分析表征.结果表明:这种技术可将具有钠米台阶的正负图形很好地传递到金膜,具有高的可信度.同时,在不同时的实验中光刻图形的重复性很好.