Near-field optical patterning and structuring based on local-field enhancement at the extremity of a metal tip

We present a particular approach and the associated results allowing the nanostructuration of a thin photosensitive polymer film. This approach based on a scanning near-field optical microscopy configuration uses the field-enhancement (FE) effect, a so-called lightning-rod effect appearing at the extremity of a metallic tip when illuminated with an incident light polarized along the tip axis. The local enhancement of the electromagnetic field straight below the tip's apex is observed directly through a photoisomerization reaction, inducing the growth of a topographical nanodot characterized in situ by atomic-force microscopy using the same probe. From a survey of the literature, we first review the different experimental approaches offered to nanostructure materials by near-field optical techniques. We describe more particularly the FE effect approach. An overview of the theoretical approach of this effect is then given before presenting some experimental results so as theoretical results using the finite-element method. These results show the influence on the nanostructuration of the polymer of a few experimental parameters such as the polarization state, the illumination mode and the tip's geometry. Finally, the potentiality of this technique for some applications in the field of lithography and high-density data storage is shown via the fabrication of nano-patterns.     

Deriving tabular event-based specifications from goal-oriented requirements models

Goal-oriented methods are increasingly popular for elaborating software requirements. They offer systematic support for incrementally building intentional, structural and operational models of the software and its environment. They also provide various techniques for early analysis, notably, to manage conflicting goals or to anticipate abnormal environment behaviours that prevent goals from being achieved. On the other hand, tabular event-based methods are well-established for specifying operational requirements for control software. They provide sophisticated techniques and tools for late analysis of software behaviour models through simulation, model checking or table exhaustiveness checks. The paper proposes to take the best out of these two worlds to engineer requirements for control software. It presents a technique for deriving event-based specifications, written in the SCR tabular language, from operational specifications built according to the KAOS goal-oriented method. The technique consists of a series of transformation steps each of which resolves semantic, structural or syntactic differences between the KAOS source language and the SCR target language. Some of these steps need human intervention and illustrate the kind of semantic subtleties that need to be taken into account when integrating multiple formalisms. As a result of our technique SCR specifiers may use upstream goal-based processes à la KAOS for the incremental elaboration, early analysis, organization and documentation of their tables, while KAOS modelers may use downstream tables à la SCR for later analysis of the behaviour models derived from goal specifications.     

Aerosol lidar intercomparison in the framework of the EARLINET project. 1. Instruments

In the framework of the European Aerosol Research Lidar Network to Establish an Aerosol Climatology (EARLINET), 19 aerosol lidar systems from 11 European countries were compared. Aerosol extinction or backscatter coefficient profiles were measured by at least two systems for each comparison. Aerosol extinction coefficients were derived from Raman lidar measurements in the UV (351 or 355 nm), and aerosol backscatter profiles were calculated from pure elastic backscatter measurements at 351 or 355, 532, or 1064 nm. The results were compared for height ranges with high and low aerosol content. Some systems were additionally compared with sunphotometers and starphotometers. Predefined maximum deviations were used for quality control of the results. Lidar systems with results outside those limits could not meet the quality assurance criterion. The algorithms for deriving aerosol backscatter profiles from elastic lidar measurements were tested separately, and the results are described in Part 2 of this series of papers [Appl. Opt. 43, 977-989 (2004)]. In the end, all systems were quality assured, although some had to be modified to improve their performance. Typical deviations between aerosol backscatter profiles were 10% in the planetary boundary layer and 0.1 x 10(-6) m(-1) sr(-1) in the free troposphere.     

An interior-point sequential approximate optimization methodology

The use of optimization in a simulation-based design environment has become a common trend in industry today. Computer simulation tools are commonplace in many engineering disciplines, providing the designers with tools to evaluate a designs performance without building a physical prototype. This has triggered the development of optimization techniques suitable for dealing with such simulations. One of these approaches is known as sequential approximate optimization. In sequential approximate minimization a sequence of optimizations are performed over local response surface approximations of the system. This paper details the development of an interior-point approach for trust-region-managed sequential approximate optimization. The interior-point approach will ensure that approximate feasibility is maintained throughout the optimization process. This facilitates the delivery of a usable design at each iteration when subject to reduced design cycle time constraints. In order to deal with infeasible starting points, homotopy methods are used to relax constraints and push designs toward feasibility. Results of application studies are presented, illustrating the applicability of the proposed algorithm.     

Potentiating effect of 5,8,11-eicosatrienoic acid on human platelet aggregation

5,8,11-Eicosatrienoic acid (20∶3ω9), a fatty acid increased in the platelet phospholipids of man and animals fed saturated fats, was either added to human platelets simultaneously with the aggregating agents, or incorporated into the platelet phospholipids by preincubation. 20∶3ω9 markedly increased the response of platelets to all aggregating agents tested when added simultaneously with the agent, but solely to thrombin and ionophore, after incorporation into the platelet phospholipids. The potentiating effects of 20∶3ω9 on thrombin aggregation do not appear to be related to prostaglandin formation, but rather to the production of a monohydroxy derivative through the lipoxygenase pathway.     

Diffraction par le réflecteur secondaire d’une antenne Cassegrain de révolution

Diffraction by the subreflector of a revolution dual reflector antenna is studied as contribution to the side-lobe radiation of the antenna; a better knowledge of this sidelobe radiation is necessary in order to satisfy more and more difficult specifications. Calculation of the subreflector diffracted field is made with geometrical theory of diffraction and more precisely with the uniform asymptotic theory. The antenna is made of two revolution surfaces generated by two meridian curves which are not necessarily the set hyperbola-parabola of the classical Cassegrainian antenna. Computation results are compared to measurements made on the subreflector of an existing antenna. The agreement is satisfying particularly in the angular zone corresponding to the primary feed spill-over.     

Synthesis and Biological Characterization of (Z)-9-Heptadecenoic and (Z)-6-Methyl-9-Heptadecenoic Acids: Fatty Acids with Antibiotic Activity Produced by Pseudozyma flocculosa

Difficulties in isolating and purifying antibiotic fatty acids from culture filtrates of Pseudozyma flocculosa, a biocontrol agent against powdery mildew, have been limiting factors in studying the properties and understanding the mode of action of the biocontrol agent. We report a new protocol for synthesizing (Z)-9-heptadecenoic and for the first time synthesis of (Z)-6-methyl-9-heptadecenoic acids, two antibiotic fatty acids produced by P. flocculosa. This allowed reproducible and quantifiable means of assaying biological activity of the molecules. In these bioassays, both molecules exhibited antifungal activity corresponding to their expected potency. These new developments should facilitate further studies aimed at deciphering the ecological properties of P. flocculosa.     

Influence of moisture content on the specific methanogenic activity of dry mesophilic municipal solid waste digestate

The objective of this study was to evaluate the influence of moisture content on the specific methanogenic activity (SMA) of a fresh dry mesophilic digestate from a municipal solid waste digester plant. For this purpose, SMA tests were performed under mesophilic conditions in 500 mL glass bottles of volume used as batch reactors, during a period of 20–25 days. Cellulose, propionate and acetate were used as substrates (5 g_COD kg^?1 digestate) at four different moisture contents, ranging from 65 to 82%. The moisture content strongly influenced the specific methanogenic activity. The highest SMA values were observed at a moisture content of 82% (11.1, 7.8 and 6.0 mg_COD g_VS^?1 d^?1 for cellulose, propionate and acetate spikes, respectively). SMA and moisture content were found to be linearly linked. Dry digestion at low water content is thus detrimental to the biological activity, probably due to physical limitations. Copyright © 2011 Society of Chemical Industry     

Phase-sensitive spatially-modulated surface plasmon resonance polarimetry for detection of biomolecular interactions

We here describe a scheme of spatially modulated surface plasmon resonance (SPR) polarimetry that enables to combine ultra-high phase sensitivity with good signal-to-noise background. The proposed approach uses spatial modulation of s-polarized component by birefringent elements and the extraction of phase-polarization information by Fourier-transform methods. This scheme was tested for monitoring the interactions between an antibody and its biological partner. Our experimental data, collected by amplitude-sensitive and phase-sensitive polarimetry demonstrate that the latter scheme provides at least one order of magnitude improvement in terms of detection limit.