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Nicole A. Turner Colin Charles Douglas A. Watkinson Eva C. Enders Geoff Klein Michael D. Rennie 《Journal of Great Lakes research》2021,47(3):614-625
Understanding patterns of fish movement in large lake ecosystems is essential for determining appropriate management actions as differences in movement behaviour can influence life history traits such as growth and survival. Lake Winnipeg in Manitoba, Canada supports the 2nd largest walleye (Sander vitreus) commercial fishery in North America. We used mark-recapture models to determine movement and estimate survival of walleye between basins of Lake Winnipeg in historical and contemporary contexts, comparing a tag-recovery study completed historically during 1974–1977 with a contemporary (2017–2019) acoustic telemetry study. Mark-recapture models revealed comparably low but detectable annual transitions between basins from historical (0.3–1.2%) and contemporary datasets (7–8.5%). Historically, fish > 300 mm more frequently moved in a south to north direction. Contemporary estimates suggest similar length-based directionality in that fish > 350 mm were always more likely to move in a south-north direction. Contemporary annual survival derived from mark-recapture models ranged between 27 and 45% and 64.3% when derived from catch curve analysis, while independently derived annual historical survival estimates ranged between 50 and 69% and 45.5% from catch curve analysis. Using the contemporary dataset, we also observed seasonal variation in movement and survival between basins, with the greatest movement across the lake occurring during the fall. Our results demonstrate a persisting pattern of low but measurable movement, suggesting between basin movement is not unusual for Lake Winnipeg. Further, low walleye survival rates reported here for the two time periods studied, support recent management actions to reduce fishing pressure across the lake. 相似文献
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Reviews the book, Counseling and Therapy Skills by David G. Martin (1983). The stated goal of this book is "to communicate more than an academic understanding of the principles of therapy: to give the reader skills that can actually be applied in the counseling setting" (p. vii). The first six chapters focus on the concept of empathy, listening skills, facilitative responding, confrontation, giving control to the client, and relationship issues. The second part book consists primarily of illustrations of therapy by five therapists (including the author). This material is followed by four chapters on the special topics of behavioural interventions, assessment, ethical issues, and how to conduct initial and final interviews. The author concludes with a theoretical section of four chapters in which he briefly discusses contributions by some of the "schools" of therapy, and then addresses the relationship between anxiety and distress, some theoretical principles underlying therapy, and the research evidence on the effectiveness of empathic responding. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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MA Pfaller S Arikan M Lozano-Chiu Y Chen S Coffman SA Messer R Rennie C Sand T Heffner JH Rex J Wang N Yamane 《Canadian Metallurgical Quarterly》1998,36(9):2609-2612
A method using a commercially prepared colorimetric microdilution panel (ASTY; Kyokuto Pharmaceutical Industrial Co., Ltd.) was compared in four different laboratories with the National Committee for Clinical Laboratory Standards (NCCLS) reference microdilution method by testing 802 clinical isolates of Candida spp. (C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. krusei, C. lusitaniae, C. guilliermondii, C. lipolytica, C. rugosa, and C. zeylanoides) against amphotericin B, 5-fluorocytosine (5FC), fluconazole, and itraconazole. Reference MIC endpoints were established after 48 h of incubation, and ASTY endpoints were established after 24 and 48 h of incubation. ASTY endpoints were determined to be the time at which the color of the first well changed from red (indicating growth) to purple (indicating growth inhibition) or blue (indicating no growth). Excellent agreement (within 2 dilutions) between the reference and colorimetric MICs was observed. Overall agreement was 93% at 24 h and 96% at 48 h. Agreement ranged from 90% with itraconazole and 5FC to 96% with amphotericin B at 24 h and from 92% with itraconazole to 99% with amphotericin B and 5FC at 48 h. The ASTY colorimetric microdilution panel method appears to be comparable to the NCCLS reference method for testing the susceptibilities of Candida spp. to a variety of antifungal agents. 相似文献
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Monomeric GART reversibly associates into a dimeric form as a function of decreasing solution pH. The transition is consistent with a three-proton transfer reaction with an apparent pKa near 7. We now report that a single mutation, which replaces a glutamic acid at position 70 in the dimer interface with alanine (E70A), disrupts the pH-dependent dimerization of GART based on dynamic light scattering and gel filtration studies. A comparison of data obtained from UV-absorbance difference spectroscopy for both the wild-type and mutant forms of GART indicates that a tyrosine residue(s) undergoes a change in solvent exposure over the pH range 6.55 to 8.19. A conformational change in tertiary structure that accompanies dimerization accounts for 60% of the observed optical difference, while the remaining 40% can be attributed to a pH-dependent process unrelated to dimerization. In addition, fluorescence studies of the mutant protein indicate that a pH-dependent change in tryptophan fluorescence exhibited by the wild-type protein is unrelated to quaternary structural changes and is likely a result of simple fluorescence quenching by nearby protonated histidine side-chains. Taken together, our results indicate that a single amino acid change at the dimer interface is sufficient to interrupt the highly specific, pH-dependent assembly reaction of GART, although pH-dependent conformational changes present in the wild-type protein also occur in E70A GART. This work is a first application of structure-based site-directed mutagenesis to the analysis of this pH-dependent assembly reaction. 相似文献
27.
Advanced interventional laparoscopy has necessitated the development of a vast array of new equipment, but inevitably some of this equipment has had to be adapted to specific patient needs. Standard laparoscopic ports may be too short for use in obese patients. We describe a technique using a Portex endotracheal tube as an over-tube, which will overcome this problem. 相似文献
28.
1. The effects of antihypertensive drugs on lipids may also influence their effect on coronary artery disease (CAD). However, the clinical significance of these effects and the extent to which they persist during long-term therapy is uncertain. 2. We performed a meta-analysis on 23 randomized trials published between 1988 and 1994 that compared the effects of atenolol, celiprolol (a beta-blocker with beta 2-adrenoceptor intrinsic sympathomimetic activity), enalapril, nifedipine and doxazosin on plasma cholesterol, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglycerides and blood pressure (BP). 3. Predicted changes in CAD risk were calculated by incorporating the results for these parameters into the Framingham equations. 4. While there were no differences in antihypertensive efficacy between the drugs, atenolol significantly (P < 0.05) reduced HDL-C and increased total cholesterol, LDL-C and triglycerides compared with celiprolol, enalapril, doxazosin and nifedipine. 5. The magnitude of the effects on lipids was not significantly influenced by the duration of therapy (up to 3 years for atenolol and doxazosin and up to 2 years for celiprolol). 6. The improvement in Framingham equation point scores (systolic BP formula) was significantly (P < 0.05) less for atenolol (-0.54; confidence intervals (CI) -0.29-(-0.78)) than for celiprolol (-1.69; CI -0.68-2.70), doxazosin (-1.67; CI -1.11-(-2.23)), enalapril (-1.43; CI 0.23-(-3.07)) and nifedipine (-1.91; CI -1.22-(-2.59)). Similar results were obtained for the Framingham diastolic BP formula. 7. These results suggest that the adverse effects of atenolol ]on plasma lipids do not improve with prolonged therapy and are theoretically great enough to reduce its efficacy in reducing CAD by approximately two thirds compared with antihypertensive drugs that do not adversely affect plasma lipids. However it must be emphasized that these are theoretical effects. In order to determine the actual differences between these drugs on CAD end points, studies using these end points are required. 相似文献
29.
Internet use and access to digital devices continues to increase even in remote regions around the world, but users do not participate equally or engage in the same practices online. This leads to inequalities in the outcomes different groups of users can generate as a result of their online practices. Drawing from recent literature on digital divides and using a theoretical framework focused on user choice and agency, we present data from a study of internet and device use in remote villages in Sarawak, a state of Malaysia on the island of Borneo. These villages lack most basic infrastructure such as paved roads or grid electricity, but some have mobile phone and mobile internet access installed under Malaysia’s Universal Service Provision. We discuss qualitative and quantitative data collected between 2015 and 2017 to point to the opportunities as well as obstacles users in remote communities encounter in their engagements with digital devices and the internet. We argue that while remote areas seem to lag behind urban areas in terms of users’ internet skills and practices, people choose to engage with these technologies in ways that are appropriate to their needs and to the local low-bandwidth environment. To enable these communities to tap into additional potential benefits of internet use, however, faster and more reliable access is a prerequisite. 相似文献
30.
Hydrostatic versus nonhydrostatic hydrodynamic modelling of secondary flow in a tortuously meandering river: Application of Delft3D
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Given the importance of pressure gradients in driving secondary flow, it is worth studying how the modelled flow structures in a natural river bend can be impacted by the assumption of hydrodynamic pressure. In this paper, the performance of hydrostatic versus nonhydrostatic pressure assumption in the three‐dimensional (3D) hydrodynamic modelling of a tortuously meandering river is studied. Both hydrostatic and nonhydrostatic numerical models were developed using Delft3D‐Flow to predict the 3D flow field in a reach of Stillwater Creek in Ottawa, Canada. An acoustic Doppler velocimeter was employed to measure the 3D flow field at a section in a sharp bend of the simulated river at two flow stages. The results of the Delft3D hydrostatic model agreed well with the acoustic Doppler velocimeter measurements: The hydrostatic model predicted reasonably accurately both the streamwise velocity distribution across the section and the magnitude and location of the primary secondary flow cell. The results of the Delft3D nonhydrostatic approximation showed that the model was not conservative and could not accurately generate either the secondary flow or the streamwise velocity distribution. This study illustrated the superior performance of the hydrostatic over nonhydrostatic 3D modelling of the secondary flow using Delft3D. Several possible reasons for unfavourable performance of the nonhydrostatic version of Delft3D are discussed, including the pressure correction technique employed in Delft3D. Considering the uncertainties that may arise in both modelling and field measurements, the 3D hydrostatic Delft3D model was capable of reasonably predicting the river bend flow structures in the studied meandering creek. 相似文献