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31.
Y Furusawa LE Quintern H Holtschmidt P Koepke M Saito 《Canadian Metallurgical Quarterly》1998,50(5):597-603
A centrifugation method was used to investigate the accumulation of 14C-rifampicin by Staphylococcus aureus and Escherichia coli, and to characterize the mechanism of rifampicin transport into S. aureus. For both species, drug accumulation was rapid with the steady-state concentration (SSC) reached within 40 s of drug exposure. Rifampicin accumulation by S. aureus was temperature and pH dependent; the lower the experimental temperature and the lower the experimental pH, the lower was the concentration of rifampicin accumulated. Accumulation was unaffected by the presence of inhibitors of antibiotic efflux, carbonyl cyanide m-chlorophenylhydrazone (CCCP), dinitrophenol (DNP), or reserpine. Exposure to increasing concentrations of rifampicin suggested that the accumulation process was saturable above a rifampicin concentration of 0.2 mg/L. Michaelis-Menten kinetics gave an apparent Km and Vmax for rifampicin, calculated from a Lineweaver-Burk plot, of 0.05 mg/L (0.06 microM) and 3.8 ng rifampicin per second, respectively. However, calculations suggest that these values reflect those for binding of rifampicin to its target, RNA polymerase. 相似文献
32.
J Rhodes A Dave MC Pulling RL Geggel GR Marx DR Fulton ZM Hijazi 《Canadian Metallurgical Quarterly》1998,81(10):1217-1219
Data from exercise tests, echocardiograms, and lung perfusion scans were analyzed to determine whether the excessive minute ventilation (VE) often encountered among patients with tetralogy of Fallot is due to ventilation-perfusion mismatch secondary to branch pulmonary artery stenoses. Patients with branch PA stenoses had lower peak oxygen consumptions and higher VE during exercise than did patients without stenoses, and a strong correlation existed between the degree of pulmonary blood flow maldistribution on lung perfusion scan and the amount of excessive VE during exercise. 相似文献
33.
AJ Conrad EY Chiang LE Andeen C Avolio SM Walker RW Baumhefner R Mirzayan WW Tourtellotte 《Canadian Metallurgical Quarterly》1994,54(1-2):99-108
A method for quantitating specific anti-viral antibodies in serum and cerebrospinal fluid (CSF) is established using enzyme-linked immunosorbent assay (ELISA). Quantitated antibody levels are used to determine intrathecal specific IgG synthesis rate for the particular antibody. Measles virus was used as a model for validating this quantitative technique: a mutated form of measles virus is a cause of subacute sclerosing panencephalitis (SSPE) and there is a possibility that measles virus is related to the cause of multiple sclerosis (MS). Matched serum and CSF samples were assayed. Concentration of anti-measles IgG was determined and intrathecal measles-specific IgG synthesis rate was calculated. For the SSPE samples, measles-specific IgG synthesis rate was elevated and comprised > 20% of the total intrathecal IgG synthesis rate; these results are consistent with the literature. The ELISA method can be performed routinely, providing a quick, simple, reproducible means of quantitating specific antibody concentrations, with sensitivity greater than 1 nanogram per milliliter. With this method, quantitation of IgG antibodies to any other viral antigen can be reliably and precisely determined. 相似文献
34.
Nonsense codons between position 14 within the first exon and position 193 within the penultimate exon of the human gene for triosephosphate isomerase reduce mRNA abundance to 25% of normal. The reduction in abundance is due to the decay of newly synthesized mRNA that copurifies with nuclei. TPI mRNA that copurifies with cytoplasm is immune to decay. We show here that immunity is not due to the failure of nonsense-containing mRNA to form polysomes. This finding indicates that cytoplasmic mRNA, in contrast to nucleus-associated mRNA, may have lost one or more factors that are required for nonsense-mediated decay or gained one or more factors that confer immunity to nonsense-mediated decay. 相似文献
35.
LE B?ttiger 《Canadian Metallurgical Quarterly》1994,91(28-29):2699-2700
36.
37.
Nianxue Song Colin Rhodes David W. Johnson Graham J. Hutchings 《Catalysis Letters》2005,102(3-4):271-279
Temperature programmed reduction (TPR) and oxidation (TPO) are used extensively in catalyst characterisation. In this paper, we examine the use of TPR/TPO cycles for the characterisation of a range of molybdates and single oxides. In particular we observe that the first cycle differs from that of subsequent analyses, even when the maximum temperature is limited to that used in the catalytic reaction. The effect is independent of heating rates and cooling atmospheres and has been demonstrated using different bed configurations. This observation has significance when these oxides are used in periodic flow reactors that involve many cyclical reduction/oxidation. 相似文献
38.
实时监控无线传感器网络(WSN)从单个节点到整个网络的运行状态,是进行无线传感器各类研究及应用开发的关键技术之一,为了克服基于UART的数据传输所存在的速率瓶颈问题,设计了一种基于USB的WSN监测系统,主要包括侦听节点和监测分析系统两部分。侦听节点采用CC2531 USB Dongle,以被动侦听的方式获取无线传感器网络的数据包(符合IEEE802.15.4标准),封装后通过USB接口上传至上位机监测分析系统。上位机监测系统,通过USB接口读取数据帧、完成帧信息存储、解析。实验结果表明该设计能够实现数据传输并以图形方式动态显示网络运行状态。本监测系统的设计为开展WSN的各类理论及实验研究提供了有力的分析工具。 相似文献
39.
片上网络模拟器的设计涉及到片上网络的拓扑结构、路由器结构、路由算法、性能分析等诸多方面.从NoC模拟器设计的角度,研究并讨论模拟器所采用的拓扑结构,路由器结构及数据包格式,介绍拓扑结构模拟、IP核模拟、路由模拟,并且用面向对象语言C++实现一个NoC模拟器系统. 相似文献
40.