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Electrospinning of protein‐loaded fibers faces many challenges, e.g. burst release owing to segregation of the protein on the fiber surface, loss of activity due to electrospinning conditions, limitation of loading capacity etc. Core–shell electrospinning provides an effective way to electrospin fibers wherein the core can be loaded with bioactive molecules in friendly conditions of a compatible polymer solution, thereby protecting the molecules from the electrostatic field and organic solvent of shell solutions. The shell polymer, after the electrospinning, acts as a barrier to control the release of the loaded molecules. However, the limitation of loading capacity still remains due the prerequisite of using an additional polymer as additive to achieve the minimum viscosity of the core solution required for viscous drag by the shell solution being drawn by the electrostatic force. The work reported here aims to alleviate the need of a polymer additive by using aqueous protein solutions of very high concentration. High concentrations of protein solutions were successfully electrospun as the core of the protein–poly(lactide‐co‐glycolic acid) core–shell fibers. A partitioning effect was seen in the controlled release of hydrophilic proteins as they were retained in the aqueous core for longer times. Using lysozyme as a model protein, it was shown that the activity is significantly retained after electrospinning, compared with electrospinning in monolithic fibers. Moreover, the lysozyme activity was also comparable with the lysozyme released from core–shell fibers spun using poly(vinyl acetate) as additive in the core. Copyright © 2012 Society of Chemical Industry 相似文献
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Gaurav Sharma Lokesh Tiwari Dheerendra Kumar Dwivedi 《Transactions of the Indian Institute of Metals》2018,71(1):11-21
In the present study, impulse pressure assisted diffusion bonding of low carbon steel was carried out using silver interlayer. To study the influence of input process parameters namely bonding temperature (T), maximum pulse pressure (P), and number of pulses (N), experiments of diffusion bonding were conducted according to the Taguchi L9 orthogonal array. To reveal the typical bond interface characteristics, selected samples were examined by scanning electron microscopy (FESEM) equipped with energy dispersive spectroscopy (EDS). The EDS analysis revealed a diffusion affected zone at the interface due to the diffusion of silver and iron across the interface. Lap joints were developed to measure the shear strength of the diffusion bonds. The optimum level of bonding temperature, maximum pulse pressure and number of pulses (875 °C, 10 MPa and 10 pulses) were identified. The ANOVA results indicated that bonding temperature had the highest statistical effect of 66.37% on shear strength followed by number of pulses and maximum pulse pressure. The fracture surface of the lap joints was also examined by FESEM and X-ray diffraction analysis. 相似文献
45.
In this paper, a machine loading problem in a flexible manufacturing system (FMS) is discussed, with bi-criterion objectives of minimising system imbalance and maximising system throughput in the occurrence of technological constraints such as available machining time and tool slots. A mathematical model is used to select machines, assign operations and the required tools in order to minimise the system's imbalance while maximising the throughput. An efficient evolutionary algorithm by hybridising the genetic algorithm (GA) and simulated annealing (SA) algorithm called GASA is proposed in this paper. The performance of the GASA is tested by using 10 sample dataset and the results are compared with the heuristics reported in the literature. The influence of genetic operators on the evolutionary search in GASA is studied and reported. Two machine selection heuristics are proposed and their influence on the quality of the solution is also studied. Extensive computational experiments have been carried out to evaluate the performance of the proposed evolutionary heuristics and the results are presented in tables and figures. The results clearly support the better performance of GASA over the algorithms reported in the literature. 相似文献
46.
Sandeep Kumar Tiwari Subbu S. Venkatraman 《Materials science & engineering. C, Materials for biological applications》2012,32(5):1037-1042
Electrospun polymeric fibers are attractive candidates in the development of scaffolds for the tissue engineering and for providing new systems for delivery of bioactive molecules. Co-axial fibers have emerged as an efficient tool to protect the core material from the adverse conditions of electrospinning process, to spin difficult-to-process fluids and to generate fibers with much more control of the delivery of encapsulated bioactive molecules. Currently, there is very little reported work on the optimization of the processing parameters of electrospinning, especially core–shell electrospinning. This study extends the understanding of the role of solution viscosity as a vital material parameter for electrospinning of fibers. The spinning solutions were characterized for viscosity and optical imaging of the compound Taylor cone for spinnability, and the fibers were imaged by Scanning Electron Microscopy (SEM). Our experimental results, using PLGA as the model polymer, confirm that the solution concentration be above the entanglement concentration (Ce) to obtain uniform beadless monolithic fibers; for core–shell fibers, the shell solution must fulfill the above criterion for spinnability and, further, the ratio of the viscosities of core and shell solutions (ηcore/ηshell) has to be greater than a threshold value to get a stable compound Taylor cone and therefore to obtain uniform beadless core–shell fibers. Addition of surfactant led to reduction of the threshold ηcore/ηshell (from 0.55 to 0.18) for the PVA–PLGA system. 相似文献
47.
Christopher Durugbo Windo Hutabarat Ashutosh Tiwari Jeffrey R. Alcock 《Journal of Intelligent Manufacturing》2012,23(5):1959-1971
In this article, the ‘information channel diagram’ (ICD) approach is introduced as a diagrammatical tool for modelling information flow during the delivery phase of organisations in which goods are deployed or delivered to customers. An initial review and evaluation of current tools for modelling information flow will be conducted based on the characteristics of information flow during the delivery phases in organisations. Diagrammatic primitives and a prescribed modelling methodology for developing an ICD will be presented, and a case scenario of the delivery phase of an organisation within the health care sector will be applied to demonstrate the use of the ICD. The article concludes by discussing some applications, generalisation potential and limitations of the ICD approach. 相似文献
48.
The machine-loading problem of a flexible manufacturing system (FMS) has been recognized as one of the most important planning problems. In this research, a Genetic Algorithm (GA) based heuristic is proposed to solve the machine loading problem of a random type FMS. The objective of the loading problems is to minimize the system unbalance and maximize the throughput, satisfying the technological constraints such as availability of machining time, and tool slots. The proposed GA-based heuristic determines the part type sequence and the operation-machine allocation that guarantee the optimal solution to the problem, rather than using fixed predetermined part sequencing rules. The efficiency of the proposed heuristic has been tested on ten sample problems and the results obtained have been compared with those of existing methods. 相似文献
49.
Curtailment of platinum catalysts loading in fuel cell is a recent central issue. As substitutes, these days several organic metal chelate compounds having featured moieties of M–N4 or M–N2O2 (M = transition metal ion) are being used as cathode catalysts in fuel cells. Here, in this study, we report in detail the electrocatalytic activity of manganese–Schiff base complexes for oxygen reduction reaction in 0·05 M HClO4 at room temperature. Actually, [Mn(salen)]+: [N,N′-bis(salicylaldehyde) ethylenediimino manganese(III)]+ and [Mn(salophen)]+: [N,N′-bis(salicylaldehyde)-1,2-phenylenediimino manganese(III)]+ were introduced into/onto the MCM-41 type silica spheres and used for the electrocatalytic reduction of oxygen. Synthesized materials were characterized by UV–Vis, FT–IR and electrochemical techniques. Significant low overpotential for oxygen reduction in 0·05 M HClO4 on [Mn(salen)]+- and [Mn(salophen)]+-incorporated silica-modified glassy carbon electrodes was observed. 相似文献
50.