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281.
    
Nonalcoholic fatty liver disease (NAFLD) is the number one cause of chronic liver disease worldwide, with 25% of these patients developing nonalcoholic steatohepatitis (NASH). NASH significantly increases the risk of cirrhosis and decompensated liver failure. Past studies in rodent models have shown that glycine-N-methyltransferase (GNMT) knockout results in rapid steatosis, fibrosis, and hepatocellular carcinoma progression. However, the attenuation of GNMT in subjects with NASH and the molecular basis for its impact on the disease process is still unclear. To address this knowledge gap, we show the reduction of GNMT protein levels in the liver of NASH subjects compared to healthy controls. To gain insight into the impact of decreased GNMT in the disease process, we performed global label-free proteome studies on the livers from a murine modified amylin diet-based model of NASH. Histological and molecular characterization of the animal model demonstrate a high resemblance to human disease. We found that a reduction of GNMT leads to a significant increase in S-adenosylmethionine (AdoMet), an essential metabolite for transmethylation reactions and a substrate for polyamine synthesis. Further targeted proteomic and metabolomic studies demonstrated a decrease in GNMT transmethylation, increased flux through the polyamine pathway, and increased oxidative stress production contributing to NASH pathogenesis.  相似文献   
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Ozonated and electrolyzed water have been reported to have bactericidal activities against most pathogenic and spoilage microorganisms associated with fresh meat and contact surfaces in meat processing facilities at room temperature. However, antimicrobial effects of these two treatments combined are not known. Therefore, the objective of this study was to evaluate the effectiveness of ozonated and electrolyzed water in combination for inactivating Escherichia coli K12 on goat meat. The combination of ozonated water and alkaline electrolyzed water resulted in higher log reductions (1.03 CFU/ml) compared to ozonated water alone (0.53 CFU/ml). Regression analysis performed using the GInaFiT tool showed that nonlinear Weibull models were more effective than log-linear models for describing the inactivation kinetics of E. coli K12 on goat meat.  相似文献   
284.
    
The total dietary fiber, sugar, protein, lipid profile, sodium, and energy contents of 11 raw cashew kernel (Anacardium occidentale L.) samples from India, Brazil, Ivory Coast, Kenya, Mozambique, and Vietnam were determined. Total fat was the major component accounting for 48.3% of the total weight, of which 79.7% were unsaturated FA (fatty acids), 20.1% saturated FA, and 0.2% trans FA. Proteins, with 21.3 g/100 g, were ranked second followed by carbohydrates (20.5 g/100 g). The average sodium content was 144 mg/kg. Fourteen FA were identified among which oleic acid was the most abundant with a contribution of 60.7% to the total fat, followed by linoleic (17.77%), palmitic (10.2%), and stearic (8.93%) acids. The mean energy content was 2525 kJ/100g. Furthermore, the sterol profile and content, amino acids, vitamins, and minerals of four raw cashew kernel samples from Brazil, India, Ivory Coast, and Vietnam were determined. β‐Sitosterol with 2380 ± 4 mg/kg fat was the most occurring sterol. Glutamic acid, with 4.60 g/100 g, was the amino acid with highest presence, whereas tryptophan with 0.32 g/100 g was the one with lower presence. Vitamin E with an average contribution of 5.80 mg/100 g was the most abundant vitamin. Potassium with a mean value of 6225 mg/kg was the mineral with highest amount in cashew samples.  相似文献   
285.
This work evaluates the rolling contact fatigue life of AISI 52100 steel bearing balls with mineral and synthetic oil, with and without additive, using a four-ball tester. IP 300/87 was applied with a total load of 600 kg (corresponding to a maximum Hertz stress of 8.709 MPa). SN-350 (a neutral mineral solvent) and TMP-05 (an environment friendly synthetic polyester) of the same viscosity were additivated with PTFE nanoparticle powder in different percentages. The total test time for each specimen was recorded and processed on Weibull probability plots. L10 and L50 are given for pure base oils and oils additivated with 1, 3 and 5 wt.% of PTFE powder. The results point to an increase in the fatigue life when additive-containing oils were used. In order to provide some understanding of the protection mechanism of the additive, the contact angle and the influence of the surface wettability on the lubricant unctuosity were analysed. Scanning electron microscopy and LINK techniques were used to study pitting, crack propagation, the chemical influence of additive and fluorine content.  相似文献   
286.
Polyalphaolefin (PAO) fluids have become widely accepted as high‐performance lubricants and functional fluids due to certain inherent, and highly desirable, characteristics. One of these characteristics is their low toxicity, which, combined with excellent viscometrics and lubricity, have made low‐viscosity PAO fluids an important component in lubricant formulations. Typical data found in product specifications for lubricants are the kinematic viscosity and the viscosity index. These values do not give enough information with which to choose the optimum lubricant for a lubricated contact. In mechanical systems, rolling, sliding, and rolling/sliding contacts occur, and lubricants have to work optimally under these operating conditions. In this study the rolling‐contact fatigue lives (L50 and L10) of PAOs of different viscosities were experimentally determined. The tests were carried out using a four‐ball machine. Wear tests were also carried out using another four‐ball tester in order to measure the wear‐scar diameter and the flash temperature parameter. The lubricants were characterised by infrared spectroscopy, and the pitting of the balls was observed using scanning electron microscopy.  相似文献   
287.
In this work, the rolling fatigue lives of three polyglycols (PAG-9, PAG-12 and BREOX-B-135X) are determined using IP-300 standard. A four-ball test machine was used and 10% life (L10) and 50% life (L50) were obtained. In addition, the stress-time curves for L10 and L50 were also determined. This work showed that: firstly, all polyglycols were tested under boundary lubrication regime (λ < 1) where in rolling contacts the surface mode of failures prevails; secondly, in oils of the same family, the pressure-viscosity coefficient is relatively constant, therefore an increase in viscosity improve the minimum film thickness with the consequent increase of the λ ratio; and finally, differences in λ ratio for the three polyglycols resulted in different asperity interactions and rolling contact fatigue lives.  相似文献   
288.
This paper presents a methodology to develop an automatic process planning system applied for scanning parts with free-form surfaces by using a laser stripe system mounted on a coordinate measuring machine (CMM). The part has been modelled using a STL format that permits the automatic recognition of any part surface. The valid orientations of the scanning device are obtained in order to guarantee the visibility of the zone to be scanned and also to be compatible with the constraints imposed by the process. With the aim to speed up the calculation of valid orientations, we apply different methods like space partitioning techniques base on kd-tree as well as back-face culling algorithms. Once the space occupied by the part is partitioned in regions, recursive ray traversal algorithms are used in order to exclusively check for intersection the part triangles of the STL model that can potentially be traversed by each laser beam direction. In order to reduce the scanning time related to laser orientation changes, part triangles must be classified into a set of clusters based on their common visibility orientations. Finally, the scanning paths for each cluster are generated as well as the joining paths between them by taking into consideration depth of field and laser beam width.  相似文献   
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Limb-girdle muscular dystrophy R1 calpain 3-related (LGMDR1) is an autosomal recessive muscular dystrophy produced by mutations in the CAPN3 gene. It is a rare disease and there is no cure or treatment for the disease while the pathophysiological mechanism by which the absence of calpain 3 provokes the dystrophy in muscles is not clear. However, key proteins implicated in Wnt and mTOR signaling pathways, which regulate muscle homeostasis, showed a considerable reduction in their expression and in their phosphorylation in LGMDR1 patients’ muscles. Finally, the administration of tideglusib and VP0.7, ATP non-competitive inhibitors of glycogen synthase kinase 3β (GSK-3β), restore the expression and phosphorylation of these proteins in LGMDR1 cells, opening the possibility of their use as therapeutic options.  相似文献   
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