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排序方式: 共有348条查询结果,搜索用时 15 毫秒
11.
S Mita M Tokunaga E Uyama T Kumamoto K Uekawa M Uchino 《Canadian Metallurgical Quarterly》1998,21(4):490-497
We examined two muscle biopsy specimens from a proband and her mother with myoclonus epilepsy with ragged-red fibers (MERRF), both obtained at an interval of about 10 years, using histochemistry, in situ hybridization, and single-fiber polymerase chain reaction. Total (wild-type and mutant) mitochondrial DNAs (mtDNAs) were greatly increased in ragged-red fibers (RRF) over non-RRF in all muscle specimens analyzed. The proportion of mutant mtDNA was also significantly higher in RRF than in non-RRF. By comparing the first and second muscle biopsied specimens in each patient, we found that while the proportion of RRF, cytochrome coxidase deficient fibers, and mutant DNA in muscle changed over a 10-year period, the proportion of wild-type and mutant mtDNAs in RRF and in non-RRF was similar between the two specimens. These results suggest that the ratio of wild-type to mutant mtDNAs in RRF and non-RRF in MERRF is at a steady state level in each muscle fiber, without replicative advantage of mutant mtDNA. 相似文献
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S Tokunaga S Morita Y Tomita Y Toshima M Masuda Y Imoto F Fukumura T Nakano R Tominaga R Kawachi H Yasui 《Canadian Metallurgical Quarterly》1996,49(13):1063-1068
Japanese Society of Cryopreserved Thoracic Tissue Implantation was recently set up. Cryopreserved allograft valves are about to pervade in Japan. To clarify the interest, demands and supply of cryopreserved allograft valve in the area of Kyushu and Yamaguchi, we performed questionnaire investigation regarding this issue. Collection rate of this questionnaire was 87.5% (35/40 hospitals). Ninety-seven percent of the hospitals answered that they were interested in cryopreserved allograft. Ninety-four percent of the hospitals answered that they did not ethically hesitate to use cryopreserved allograft. Ninety-one percent of the hospitals hoped to perform cardiac surgery using allograft aortic valve if allograft is available. With respect to securing donors of allograft, two-third of the hospitals did not decide whether they could be involved in obtaining donors of allograft. As the number of the cadaver kidney donors is about 20 per year in the area of Kyushu and Yamaguchi, shortage of donors of allograft valve is anticipated. 相似文献
14.
N Keicho K Tokunaga K Nakata Y Taguchi A Azuma M Bannai M Emi N Ohishi Y Yazaki S Kudoh 《Canadian Metallurgical Quarterly》1998,158(3):846-850
Diffuse panbronchiolitis (DPB) in East Asia is a distinctive chronic obstructive pulmonary disease of unknown etiology. We hypothesize that the disease susceptibility is due to genetic predisposition unique to Asians. Association between human leukocyte antigen (HLA)-Bw54 and the disease was previously reported. In the present study, using newly developed polymerase chain reaction (PCR)- based methods, we directly analyzed HLA class I and II alleles in 76 Japanese patients. HLA-A, -B, and -C antigens were screened by the conventional typing method, and then B22-group alleles including HLA-B54 were genotyped by single-strand conformation polymorphism analysis. Alleles of HLA-DRB1 gene were fully determined by the microtiter plate hybridization method. Thirty-seven percent of the patients possessed HLA-B*5401 allele conserved predominantly in East Asians, as compared with 15% of 110 healthy volunteers (chi2 = 12.4, p = 0.0004). In addition, 4% of the patients possessed B*5504 also unique to Asians but a rare allele which was not found in normal control subjects in this study. Typing of HLA-DRB1 class II gene did not demonstrate strong positive association with the disease. A33, B44, and DRB1*1302 showed negative association with the disease. We conclude that distinctive molecular structure of HLA-B alleles or a closely linked gene in the HLA region contributes to genetic predisposition in diffuse panbronchiolitis. This may partly explain why this disorder is found primarily in Asians. 相似文献
15.
Volcanic disasters can cause severe loss of human life and damage to property. The main damage is caused during an eruption and from subsequent erosion of deposited materials. Heavy rainfall in volcanic areas erodes volcanic deposits, mainly pyroclastic flows and ash fall deposits, which flow as lahar to the foothill of the mountain and cause drastic damage to economically important areas. This post-eruption disaster becomes complex due to the occurrence of stream captures and watershed breakouts that lead to devastating lahars. Continuous monitoring of such geomorphic and hydrologic changes is necessary to cope with changing hazard conditions. Therefore it is important to update the watershed boundaries in order to study current hazard conditions and develop mitigation plans for future disasters. Changes of geomorphic and watershed boundary have occurred in the Mayon Volcano in the Philippines mainly as a result of a major volcanic eruption of 1993, due to which mitigation structures were constructed and modified in the low lying areas. In this study interferometry was used to develop DEM from SAR data to delineate watershed boundaries. New lava flows, pyroclastic flows and lahar deposits in the watersheds were mapped using elevation changes, coherence and intensity derived from the SAR images. Updating geomorphic features of the watersheds and their boundaries using SAR provides a new weather independent alternative technique for monitoring the effect of volcanic activity. 相似文献
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Hiraiwa A. Shimohara K. Tokunaga Y. 《IEEE engineering in medicine and biology magazine》1990,9(3):39-42
Electroencephalography (EEG) pattern-recognition studies were carried out using EEG topography (readiness potential, or RP, spatiotemporal patterns) generated the moment before voluntary movements of muscles. RPs generated prior to pronouncing syllables and controlling a joystick were studied by experiments and simulation. The spatiotemporal patterns of RPs were measured by multichannel surface electrodes pasted on the subject's scalp. Backpropagation neural networks were used for RP pattern recognition. The results show that RPs generated prior to syllable pronouncement contain some information about those syllables, and that RPs generated prior to joy stick movements contain information on the direction of intended movement. They also show that neural networks can be used to recognize EEG information and so create a new type of man-machine interface for data input. 相似文献
18.
S. Fujino H. Tokunaga S. Hata E. Saiz A. P. Tomsia 《Journal of Materials Science》2005,40(9-10):2499-2503
A graded glass coating for Vitallium®, a Co-Cr alloy, has been prepared using a simple enameling technique. The composition of the glasses has been tailored to match the thermal expansion of the alloys. The optimum glass composition and firing conditions (temperature and time) needed to fabricate homogeneous coatings with good adhesion to the alloy were determined. The final coating thickness ranged between 25 and 60 μm. Coatings fired under optimum conditions do not delaminate during indentation tests of adhesion. Excellent adhesion to the alloy has been achieved through the formation of 100 nm thick interfacial chromium-oxide (CrOx) layers. The graded glass (consisting of BIG and 6P50 layers) can be successfully coated to a Co-Cr alloy, and forms hydroxyapatite (HA) on the coating surface when immersed in a simulated body fluid (SBF) for 30 days. 相似文献
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Hardware encryption engines are essential components of secure systems. They are widely used in desktop applications such as the trusted platform module as well as in mobile applications where they offer high energy efficiency compared to their software implementation counterparts. Unfortunately, ASIC encryption engines leak side-channel information through their power supplies. This information can be used by attackers to reveal their secret keys with attacks such as differential power analysis. Dual-rail logic and noise addition circuits increase the security against these attacks, but they add higher than 3x overheads in area, power, and performance to unsecured encryption engines. In this work, we present a switched capacitor circuit that equalizes the current to isolate the critical encryption activity from the external supplies, eliminating the side-channel information leakage. The secure encryption system was implemented in a 0.13 ?m CMOS technology with 7.2% area and 33% power overheads and a 2 × performance degradation. The secret encryption key was not revealed after ten million side-channel attacks. 相似文献