The distribution of anthocyanins in grape skin extracts of port wine cultivars as determined by high performance liquid chromatography

The distribution of anthocyanins in methanolic skin extracts of 16 grape cultivars used for port wine production and grown at five sites in the Douro Valley in Northern Portugal has been assessed by high performance liquid chromatography. Results are confined to the seven most readily separated and identified anthocyanins and are expressed as percentages of their total (88–99% of the total integrated area). Anthocyanins based on malvidin (Mv) predominated. Of these, Mv 3-glucoside was the major pigment (33–60%), being exceeded by Mv 3-p-coumarylglucoside (2–51%) in only two cultivars; Mv 3-acetylglucoside (1–15%) was consistently the lowest. Peonidin 3-glucoside (1–27%) was prominent in four cultivars, but delphinidin 3-glucoside (1–11%), petunidin 3-glucoside (2–11%) and cyanidin 3-glucoside (trace-6%) were of low proportions throughout. The ratio Mv 3-acetylglucoside/total Mv glucosides appeared characteristic of cultivar, independent of site, and a useful aid to identification of grape cultivars. The coloured pulp of some cultivars contained peonidin 3-glucoside as a major component, present in greater proportions than in the skin; the percentage of Mv 3-p-coumarylglucoside was lower in pulp than in skin. The identities of six of the seven anthocyanins were confirmed by fast atom bombardment mass spectrometry and the structures of the Mv derivatives were determined by nuclear magnetic resonance. The formation of formyl and acetyl anthocyanin artefacts is described.     

On the calculation of solar radiation in hazy atmospheres and on turbidity in Ibadan

A previous model is examined in greater detail for the harmattan haze periods (November to January) of 1975–1980 at Ibadan, when the atmosphere is often heavily overcast with dust. It is shown that for air mass (m) and turbidity (β) product values of mβ > 4.05 the scattering transmittance τ_scat, for the instantaneous direct solar radiation becomes negative, while for mβ < 0.22 τ_scat changes too rapidly, thus failing to give acceptable values of irradiation for such periods. This problem is overcome by employing the limits 0.22 mβ 3.07 in the calculations, which corresponds to β of 0.40 and 0.22 for “very hazy” and “hazy-hot clear” atmospheres, respectively. The resulting daily and monthly average total insolation are mainly within ± 15% and ± 10%, respectively, of the experimental data provided by the International Institute of Tropical Agriculture (I.I.T.A.), Ibadan.The variation of the turbidity in the city is also studied for all days of the six years. Daily average values 0.10 β 0.175 occur on about 60% of the days, while “clear” (β < 0.125) and “very hazy” (β 0.40) weather days have about 16% and 12.37%, respectively. Some 74.4% of the very hazy days fall within the harmattan period during which the monthly average turbidity is often higher than 0.35.     

Heat loss and thermoelectric generator design

With the object of evaluating its importance to thermoelectric generator design, heat loss is introduced into the standard thermoelectric generator design theory. The theory for both the constant hot and cold junction temperatures model and the constant heat input model are so modified. The modification is first order and, therefore, is limited to small leg heat-transfer coefficients. Numerical results using representative properties show that significant differences can exist between the optimum geometry and performance of a generator idealized as lossless and those of a generator designed by the modified theory. The largest differences occur with the constant heat input model.     

Effect of vitamins A, E and K on the indices of the glutathione antiperoxide system in gingival tissues in periodontosis

Glutathione reductase is activated and the content of glutathione sulfhydryl groups is increased in the gingival tissue of patients afficted with parodontosis. The degree of alterations depends on the degree of the development and character of the disease. Application of antioxidant vitamin therapy (vitamins A, E and K) locally and per os normalizes the parameters under study and improves the status of the parodontium.     

Maternal and congenital syphilis

Testing for syphilis during pregnancy reveals a positive serologic status in 0.02% of cases. However, a 66% rate of stillbirths is noted in women who are infected and who have not benefited from any treatment. Routine screening is at present performed during the early stages of pregnancy but a second serologic test during the third trimester is useful in the diagnosis of a late infection especially in drug users or HIV (human immunodeficiency virus) positive patients. Congenital syphilis is diagnosed in utero when a positive maternal serologic status is associated with ultrasound images showing fetal abnormalities; these include hepatosplenomegaly, hyperechogenic bowel, signs of bowel obstruction or fetal hydrops. Maternal syphilis is treated by delayed action penicillin and is indicated even for patients allergic to the antibiotic which in this particular case is delivered after desensitization. First line therapy by intravenous penicillin is indicated when confronted with the following high risk factors of congenital syphilis: an elevated titre of VDRL (venereal disease research laboratory) at the time of diagnosis or delivery, unknown date of the precise onset of the infection, the appearance of a rash or of a chancre during pregnancy, ultrasound fetal abnormalities or late therapy during the third trimester. Treatment of the new-born child will depend on the results of clinical, serologic and X-ray evaluation. Long term follow-up for at least a year is mandatory.     

Properties of slips of zirconia stabilized by oxides of rare earth metals

Casting properties of concentrated aqueous dispersions of zirconia stabilized by Y₂O₃, Si₂O₃, or a mixture of them are investigated. Optimum values of the pH, temperature, and slip concentration for the production of thin-walled vacuum-tight articles are established. Translated from Ogneupory, No. 3, pp. 12–15, March, 1995.     

Electrodynamic headphones and woofers for application in magnetic resonance imaging scanners

Electrodynamic speakers compatible with (functional) magnetic resonance imaging (MRI) are described. The speakers magnets are removed, their function is replaced by the scanner's magnetic field, resulting in an uncommon but efficient operation. The method can be used with headphones as well as woofers. Functional MRI is not associated with any known biological risks, but as a method for visualization of task-specific activation of brain regions it is undesirably noisy. Thus, it requires both noise protection and efficient sound transmission systems for delivering acoustic stimuli to subjects. Woofers could possibly be used in active noise-control systems. The speakers described in this paper can be used for either task.