Optimization of fermentation condition for co-production of ethanol and 2,3-butanediol (2,3-BD) from hemicellolosic hydrolysates by Klebsiella oxytoca XF7

Microbial production of ethanol and 2,3-butanediol (2,3-BD) from agro-residues has been attracting interest because of their applications in various industries, including generation of biofuel molecules. In the present investigation, the hemicellulosic fraction of corncob was hydrolyzed by indigenous holocellulase from novel psychrotolerant Aspergillus niger SH3 resulting in high xylose release (34.61?g?L^?1), followed by the bioconversion of xylose to ethanol and 2,3-BD. Taguchi design was adopted to optimize the process which resulted in 5.25- and 3.31-fold increase in 2,3-BD (12.18?±?0.53?g?L^?1) and ethanol (4.08?±?0.03?g?L^?1), as compared with un-optimized condition. For the first time, co-production of ethanol and 2,3-BD from the corncob hemicellulosic hydrolysate was performed using a newly isolated Klebsiella oxytoca XF7 strain, under the optimized fermentation conditions. These results suggest that K. oxytoca XF7 is a promising candidate for co-production of ethanol and 2,3-BD, with high xylose conversion efficiency (96.65%), facilitating the economical production of biofuel molecules.     

Study of heat and mass transfer in indoor conditions for distillation

An indoor simulation study was carried out to evaluate heat and mass transfer relation for a semi-cylindrical metallic (opaque) condensing cover for higher yields at different operating temperatures under free and forced modes of operation. The objective is to design a distillation unit for higher yield for commercialization, particularly in India. Experiments have been conducted for the operating temperature range of 40°C to 80°C in a steady-state condition by using constant temperature bath. Data (temperature and yield) obtained from experimentation have been used to determine the values of coefficient C and n and, consequently, convective as well as evaporative heat transfer coefficients. It is inferred that a higher yield is obtained with an increase of temperature in the forced mode of operation as compared to that in the natural mode of operation due to a fast release of heat from the condensing cover.     

Synthesis of highly pure and dense 0.9(KNbO3)-0.1(BaNi1/2Nb1/2O3-δ) ceramic with superior magnetic properties

Residual NiO phase is generally detected in 0.9(KNbO₃)–0.1(BaNi_1/2Nb_1/2O_3-δ) (KBNNO) synthesized using NiO as a nickel precursor by solid-state reaction. In this work, NiO phase is found to exist in the form of the residual NiO particles with a size of 100-200 nm using energy dispersive X-ray elemental mapping. These NiO residual particles are eliminated by using nickel acetate as a nickel precursor and a 100% perovskite phase KBNNO is successfully synthesized at as low as 600°C temperature. Furthermore, using the two-step sintering technique, 100% relative density is achieved in this material. The nickel acetate–based KBNNO shows a robust ferromagnetism with the saturation magnetization of 11.42 memu/g and the remanent magnetization of 3.89 memu/g which is 38 times higher than that of previously reported value in NiO-based KBNNO. Thus, a highly pure and fully dense KBNNO ceramic with superior magnetic properties is obtained using nickel acetate and by the two-step sintering method. This is a key step forward in the processing of KBNNO and is likely to have a significant impact on other physical properties of this newly invented and promising photovoltaic perovskite material.     

In vitro biocompatibility and antimicrobial activity of chitin monomer obtain from hollow fiber membrane

This study for the first time shows the effective utilization and production of chitin monomers at laboratory level, with immense potential for its biomedical application. Low molecular weight (LMW) N-acetylglucosamine (GlcNAc) is prepared by depolymerization of chitin using chemical method coupled with a physical separation method. A novel filtration strategy exploiting polysulfone hollow fiber membrane is used for the preparation of GlcNAc particles with 94% yield within 8.5 ± 0.5 h. This high efficiency is analyzed using high-pressure liquid chromatography. The GlcNAc obtained was further analyzed using dynamic light scattering, first derivative Fourier transform infrared spectroscopy, and X-ray diffraction techniques. The antimicrobial properties of GlcNAc, chitin, and GlcNAc/chitin mixture were investigated using minimal inhibitory concentration against S. aureus and E. coli. Bacteriostatic property was exhibited by high molecular weight chitin, while GlcNAc and GlcNAc/chitin mixture (LMW) demonstrated bactericidal activity. Blood biocompatibility below 0.25 g/ml and cytocompatibility with NIH3T3 fibroblast cells and the proliferative efficacy suggested its utilization and suitability of these particles in biological applications.     

The development of low-temperature hardening silicone ceramer coatings for the corrosion protection of metals

The process parameters for the formulation of two different hybrid ceramic-polymer (ceramer), barrier-type coatings for the corrosion protection of metals are discussed. The coating formulations were characterized in the liquid, gel, and solid phases using Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy and Secondary Ion Mass Spectroscopy (SIMS), respectively. The coatings were applied to 6061-T6 aluminum alloy, hardened in ambient conditions, and analyzed with X-ray photoelectron spectroscopy (XPS). Thermal stability of the coatings was analyzed in inert and oxidative atmosphere using thermogravimertic analysis. The final surface morphology was studied using Scanning Electron Microscope (SEM) and Atomic Force Microscopy (AFM). The nanoindentation technique supported by SEM revealed the nanomechanical properties of the coatings. The effectiveness as a barrier-type coating was studied using electrochemical polarization experiments and immersion experiments in saline solution.     

A passive auxiliary circuit achieves zero-voltage-switching in full-bridge converter over entire conversion range

A passive auxiliary circuit is proposed to achieve zero-voltage-switching (ZVS) over the entire conversion range in a full-bridge (FB) pulse-width modulated (PWM) converter (FBZVS converter) with minimum conduction loss penalty. The stored energy in the auxiliary circuit is minimal under the full-load condition. It increases progressively as the load current decreases. The proposed auxiliary circuit is passive, simple and can be viewed as an add-on to the conventional FBZVS converter. The principle of operation is described and the performance is demonstrated on a 100 kHz, 500 W prototype.     

Quantile estimation based on nomination sampling

Nomination sampling is a sampling process in which every observation is the maximum of a random sample from some distribution. If all samples are taken from a single underlying CDF, F, data can be viewed as consisting of pairs (X_i,K_i) where K_i is the size of sample i and, given K_i=k_i, X_i is distributed according to CDF F^ki. R.A. Boyles and F.J. Samaniego (1986) developed a nonparametric maximum-likelihood estimator of F. In the present work, their approach is extended to obtain estimates of the quantiles of F and to study the limit theory and consistency properties of these estimates. These results generalize the results of T.R. Willemain (1980), who discussed the estimation of the median of F based on nomination samples     

Analysis of a solar water heating system with n-tanks in series

A straightforward analysis of a solar water heating system with n-tanks connected in series has been presented. The long-term performance of the system has also been studied. On the basis of numerical calculations made for four successive days, the following conclusions have been drawn:

1. (1) The fluctuation in temperature variation decreases with increase of the number of tanks connected in series.

2. (2) The variation becomes smooth after the second successive day, which is more desirable from the point of view of users.

     

Life cycle cost analysis of HPVT air collector under different Indian climatic conditions

In this communication, a study is carried out to evaluate an annual thermal and exergy efficiency of a hybrid photovoltaic thermal (HPVT) air collector for different Indian climate conditions, of Srinagar, Mumbai, Jodhpur, New Delhi and Banglore. The study has been based on electrical, thermal and exergy output of the HPVT air collector. Further, the life cycle analysis in terms of cost/kWh has been carried out. The main focus of the study is to see the effect of interest rate, life of the HPVT air collector, subsidy, etc. on the cost/kWh HPVT air collector. A comparison is made keeping in view the energy matrices. The study reveals that (i) annual thermal and electrical efficiency decreases with increase in solar radiation and (ii) the cost/kWh is higher in case of exergy when compared with cost/kWh on the basis of thermal energy for all climate conditions. The cost/kWh for climate conditions of Jodhpur is most economical.