Interferon-gamma and interleukin-10 inhibit antigen presentation by Langerhans cells for T helper type 1 cells by suppressing their CD80 (B7-1) expression

CD80(B7-1) and CD86(B7-2) co-stimulatory molecules have been reported to activate Th1/Th2 development pathways differentially. It is well known that Langerhans cells (LC), potent antigen-presenting dendritic cells in the epidermis, express several co-stimulatory molecules and that this expression is modulated by several cytokines. Based on the recently reported effect of interferon (IFN)-gamma and interleukin (IL-)-10 on the expression of CD80 and CD86 by LC, we examined the effects of these cytokines on the expression of CD54 (intercellular adhesion molecule-1) and CD40 in addition to CD80 and CD86 on LC, and correlated the expression of each co-stimulatory molecule with antigen presentation for a Th1 clone by cultured LC (cLC) treated with these cytokines. LC cultured for 72 h significantly up-regulated MHC class II antigen expression and all the co-stimulatory molecules were examined. As previously reported, IL-10 or IFN-gamma inhibited the up-regulation of CD80 expression. Granulocyte/macrophage-colony-stimulating factor (GM-CSF) partially restored the suppression of CD80 expression induced by IFN-gamma on cultured LC, while it had virtually no effect on the inhibition induced by IL-10. Antigen presentation for the myoglobin-specific syngeneic Th1 clone by cLC, which were pre-incubated with these cytokines, correlated well with their CD80 expression. In addition, among the antibodies for CD80, CD86, CD28 or CD40, the suppression of the Th1 clone stimulation by LC was found to occur only with anti-CD80 and anti-CD28 antibodies. Finally, we studied the effects of IFN-gamma and IL-10 on GM-CSF production by epidermal keratinocytes (KC). We could show that only IFN-gamma, but not IL-10, suppressed GM-CSF production by KC. These findings suggest that both IFN-gamma and IL-10 suppress antigen presentation by LC for Th1 cells by suppressing their CD80 expression. The inhibitory effect of IFN-gamma on CD80 expression on LC appears to be partially mediated through the suppression of GM-CSF production by KC.     

Morphological variations of the human gastric mucosa after omeprazole treatment: a scanning electron microscopic study

The effects of antiepileptic drugs on cognitive function in 48 healthy volunteers were assessed using event-related potentials (ERP) and the Attention Index included in the Wechsler Memory Scale, revised edition (WMS-R). The study was conducted over 1 week, using a double-blind design. Four drugs, carbamazepine (CBZ), phenytoin (PHT), valproate (VPA) and zonisamide (ZNS) were tested. Using an auditory oddball task, ERP measurements were made under two conditions with different tone intensities: Condition 1 used 70 db SPL; and Condition 2 used 30 db SPL. Results showed that CBZ prolonged target N1 and P3 latencies in Condition 1, and reduced frequent N1 amplitude in Condition 2, which suggests that CBZ may cause a change in sensory memory and prolong stimulus evaluation time. It is suggested that under a low stimulus intensity level, the sensory function itself was affected. Phenytoin was found to prolong target N1 latency in Condition 2, which also indicates a change in the sensory memory function. However, VPA did not significantly affect ERP components, except for the shortened frequent N1 latency, which could not be explained due to the limited information. It was found that ZNS augmented P3 amplitude in Condition 2, and reduced scores on the Attention Index. It is suggested that the augmentation of P3 amplitude was caused by the reduction of processing negativity as a result of the detrimental effect of ZNS on subjects' attention. However, the apparent difference between the ERP and behavioral indices suggests that caution should be exercised in assessing the results obtained only from ERP measurements.     

A new minimum zone method for evaluating flatness errors

A new minimum zone method for flatnes error analyis is proposed in this article. Based on the criteria for the minimum zone solution and strict rules for data exchange, a simple and rapid algorithm, called the control plane rotation scheme, is developed for the flatness analysis of a flat surface. Experimental work was performed, and some examples are given in terms of the minimum zone and least-squares solutions.     

Heterotopic replantation

In multidigital amputations, it is sometimes better to replant an amputated finger to a different proximal part if a better function can be expected in this position. In our clinical material between October 1991 and March 1994, heterotopic replantation was performed in twelve digits in eleven patients. Three fingers were replanted to the thumb, three to the index, four to the middle, and two to the ring fingers. The functional results were satisfying. The total active range of motion was on an average 24% of a normal finger. Static two-point discrimination was 8.5 mm on an average, and the values for the Semmes-Weinstein test ranged between 3.61 and 6.5. A heterotopic replantation is of special value for primary thumb reconstruction.     

A methodology for the performance evaluation of data querystrategies in universal mobile telecommunication systems (UMTS)

The anticipated emergence of third-generation mobile systems, referred to as universal mobile telecommunication systems (UMTS), raises the problem of reconsidering the design of the databases destined to contain the user information. In particular, it is expected that the key concepts of the new database architectures will be high distribution and fast updating of information. So far, the problem of determining the most appropriate distributed database (DDB) architectures for third-generation mobile systems has not been widely dealt with in literature. This paper presents a methodology for evaluating hierarchical DDB architectures by means of an analytical model of the data querying operation. This methodology allows for structural alternatives, differing on account of the number of levels and branches in the hierarchy, to be evaluated in terms of query loads and mean response times, according to a given user mobility characterization and a given search protocol operation. By way of illustration, the paper discusses a case study, concerning a query operation arising from a location updating procedure and applied to a hierarchical tree-like DDB in which some structural alternatives are considered     

Transition metal joints for steam generators—An overview

The transition metal joint (TMJ) between an austenitic stainless steel and a chromium-molybdenum (Cr-Mo) ferritic steel used widely in steam generators of power plants has for a long time presented problems relating to premature failures in service. The direct (bimetallic) TMJ presently in use is designed for a service life of about 200,000 h; but such TMJs with iron-base weld metals have been failing in service within about one-third of their design lifetime, while their counterparts with nickel-base weld metals fail within about one-half of their design lifetime. The causes for such premature service failures of these TMJs are discussed in detail, leading to the development of improved TMJs. One of the improved TMJs with a trimetallic configuration of austenitic stainless steel/Alloy 800/Cr-Mo ferritic steel is discussed in detail, covering its development, characterisation and evaluation. Accelerated performance tests in the laboratory have indicated a four-fold improvement in the service life of the TMJ with this trimetallic configuration compared to the bimetallic configuration. The metallurgical details of these studies are also discussed in this paper.     

Computer processing of artifact and arrhythmias in heart rate variability analysis.

Analysis of heart rate variability (HRV) with Holter monitoring is often difficult due to excessive artifacts and arrhythmias. While short sudden surges are treated successfully by most methods, slow heart rate (HR) variations, nocturnal trapezoidally-shaped HR increases and special types of arrhythmias which are similar to normal HRV fluctuations may distort further time domain and spectral analysis. This paper examines the advantages and disadvantages of different methods for preprocessing of HR data. We have developed the following approach to the analysis of HRV. (1) A combination method based on the absolute difference between HR values and both the last normal HR value and an updated mean is used for removal of artifacts and arrhythmias. This method can detect both sudden surges in HR values as well as longer periods of noise combined with slow normal variations. An additional stage of wild point removal is then optionally applied. (2) Certain special problems such as large T-waves, bigeminal rhythm, slow HR variations and nocturnal trapezoidally-shaped HR increases are also identified. Although none of the algorithms can be applied successfully to all cases, the final computer analysis for preprocessing described in the present study has proved to be superior to the simplified methods which are usually used and provides more suitable data for HRV analysis.     

A typed functional extension of logic programming

A logic language is suitable for specification if it is equipped with features for data abstraction and modularization. In this paper, an effective mechanism to incorporate function and type into logic programming is presented as the means to embed data abstraction mechanism into logic programming. This incorporation is essentially based on Horn clause logic with equality and a polymorphic type system that is an extension of Mycroft and O’Keefe’s system. This paper also presents an implementation based on Warren Abstract Machine (WAM) and shows the performance, along with a comparison with WAM.     

Adaptive nonlinear cancellation for high-speed fiber-optic systems

The authors propose techniques for adaptive nonlinear cancellation of intersymbol interference (ISI) in the electrical signal at the receiver in Gb/s lightwave systems and describe several demonstrations of these techniques. Techniques for adjustable nonlinear cancellations are discussed and demonstrations of these techniques using commercially available integrated circuits (ICs) at data rates as high as 1.7 Gb/s are described. Techniques for automatic adjustment are discussed, and a demonstration of adaptive nonlinear cancellation at 450 Mb/s is described. The authors discuss how these techniques can be integrated onto the detector IC for operations at 2.5 Gb/s and higher data rates. These techniques allow a single IC detector with adaptive nonlinear cancellation to be used in long-haul and undersea lightwave systems to optimize the detector threshold and compensate for the ISI