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991.
This paper presents a real options model where multiple options are evaluated simultaneously so that the effect of the individual options on each other is accounted for. We apply this model to the electricity sector, where we analyze three typical technologies based on fossil fuel, fossil fuel with carbon capture and renewable energy, respectively. In this way, we can analyze the transition from CO2-intensive to CO2-neutral electricity production in the face of rising and uncertain CO2 prices. In addition, such a modelling approach enables us to estimate precisely the expected value of (perfect) information, i.e. the willingness of investors and producers to pay for information about the correct CO2 price path. As can be expected, the expected value of information rises with increasing CO2 price uncertainty. In addition, the larger the price uncertainty, the larger are the cumulative CO2 emissions over the coming century. The reason for this is that the transition to less CO2-intensive technologies is increasingly postponed with rising CO2 price uncertainty. By testing different price processes (geometric Brownian motion versus jump processes with different jump frequencies), we can also make useful recommendations concerning the importance of policy predictability. We find that it is better to have climate change policies that are stable over a certain length of time and change abruptly than less abrupt but more frequently changing policies. Less frequent fluctuations reduce the expected value of information and result in smaller cumulative CO2 emissions. 相似文献
992.
Farbod Sedaghat-Hamedani Sabine Rebs Ibrahim El-Battrawy Safak Chasan Tobias Krause Jan Haas Rujia Zhong Zhenxing Liao Qiang Xu Xiaobo Zhou Ibrahim Akin Edgar Zitron Norbert Frey Katrin Streckfuss-Bmeke Elham Kayvanpour 《International journal of molecular sciences》2021,22(23)
Introduction: Familial dilated cardiomyopathy (DCM) is clinically variable and has been associated with mutations in more than 50 genes. Rapid improvements in DNA sequencing have led to the identification of diverse rare variants with unknown significance (VUS), which underlines the importance of functional analyses. In this study, by investigating human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs), we evaluated the pathogenicity of the p.C335R sodium voltage-gated channel alpha subunit 5 (SCN5a) variant in a large family with familial DCM and conduction disease. Methods: A four-generation family with autosomal dominant familial DCM was investigated. Next-generation sequencing (NGS) was performed in all 16 family members. Clinical deep phenotyping, including endomyocardial biopsy, was performed. Skin biopsies from two patients and one healthy family member were used to generate human-induced pluripotent stem cells (iPSCs), which were then differentiated into cardiomyocytes. Patch-clamp analysis with Xenopus oocytes and iPSC-CMs were performed. Results: A SCN5a variant (c.1003T>C; p.C335R) could be detected in all family members with DCM or conduction disease. A novel truncating TTN variant (p.Ser24998LysfsTer28) could also be identified in two family members with DCM. Family members with the SCN5a variant (p.C335R) showed significantly longer PQ and QRS intervals and lower left ventricular ejection fractions (LV-EF). All four patients who received CRT-D were non-responders. Electrophysiological analysis with Xenopus oocytes showed a loss of function in SCN5a p.C335R. Na+ channel currents were also reduced in iPSC-CMs from DCM patients. Furthermore, iPSC-CM with compound heterozygosity (SCN5a p.C335R and TTNtv) showed significant dysregulation of sarcomere structures, which may be contributed to the severity of the disease and earlier onset of DCM. Conclusion: The SCN5a p.C335R variant is causing a loss of function of peak INa in patients with DCM and cardiac conduction disease. The co-existence of genetic variants in channels and structural genes (e.g., SCN5a p.C335R and TTNtv) increases the severity of the DCM phenotype. 相似文献
993.
Kvin Mairot Vasily Smirnov Batrice Bocquet Gilles Labesse Carl Arndt Sabine Defoort-Dhellemmes Xavier Zanlonghi Dalil Hamroun Danile Denis Marie-Christine Picot Thierry David Olivier Grunewald Mako Pgart Hlna Huguet Anne-Franoise Roux Vasiliki Kalatzis Claire-Marie Dhaenens Isabelle Meunier 《International journal of molecular sciences》2021,22(23)
Pathogenic variants in CRB1 lead to diverse recessive retinal disorders from severe Leber congenital amaurosis to isolated macular dystrophy. Until recently, no clear phenotype-genotype correlation and no appropriate mouse models existed. Herein, we reappraise the phenotype-genotype correlation of 50 patients with regards to the recently identified CRB1 isoforms: a canonical long isoform A localized in Müller cells (12 exons) and a short isoform B predominant in photoreceptors (7 exons). Twenty-eight patients with early onset retinal dystrophy (EORD) consistently had a severe Müller impairment, with variable impact on the photoreceptors, regardless of isoform B expression. Among them, two patients expressing wild type isoform B carried one variant in exon 12, which specifically damaged intracellular protein interactions in Müller cells. Thirteen retinitis pigmentosa patients had mainly missense variants in laminin G-like domains and expressed at least 50% of isoform A. Eight patients with the c.498_506del variant had macular dystrophy. In one family homozygous for the c.1562C>T variant, the brother had EORD and the sister macular dystrophy. In contrast with the mouse model, these data highlight the key role of Müller cells in the severity of CRB1-related dystrophies in humans, which should be taken into consideration for future clinical trials. 相似文献
994.
Wolfgang E. Huhn Frank Hollmann Sabine Hild Thomas Kriewall Bernhard Rieger 《大分子材料与工程》2000,283(1):115-119
The present paper reports on a novel family of ethene/CO‐propene/CO‐block terpolymers, with propene/CO contents up to 75 mol‐%. These materials are not accessible by conventional copolymerization protocols, due to the high rate of polymerization of E/CO relative to P/CO. Therefore, a new polymerization procedure was developed that comprises a discontinuous addition of ethene/CO to an active propene/CO copolymerization reaction (“pulse‐feed‐polymerization”). This strategy affords the formation of block‐copolymers with precise control over the crystallizable ECO segments, leading to excellent thermoplastic elastomers. Synthesis, structural characterization and some of the mechanical properties of this new materials are discussed. 相似文献
995.
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998.
Farbod Sedaghat-Hamedani Sabine Rebs Elham Kayvanpour Chenchen Zhu Ali Amr Marion Müller Jan Haas Jingyan Wu Lars M. Steinmetz Philipp Ehlermann Katrin Streckfuss-Bmeke Norbert Frey Benjamin Meder 《International journal of molecular sciences》2022,23(20)
Dilated cardiomyopathy (DCM) is a common cause of heart failure (HF) and is of familial origin in 20–40% of cases. Genetic testing by next-generation sequencing (NGS) has yielded a definite diagnosis in many cases; however, some remain elusive. In this study, we used a combination of NGS, human-induced pluripotent-stem-cell-derived cardiomyocytes (iPSC-CMs) and nanopore long-read sequencing to identify the causal variant in a multi-generational pedigree of DCM. A four-generation family with familial DCM was investigated. Next-generation sequencing (NGS) was performed on 22 family members. Skin biopsies from two affected family members were used to generate iPSCs, which were then differentiated into iPSC-CMs. Short-read RNA sequencing was used for the evaluation of the target gene expression, and long-read RNA nanopore sequencing was used to evaluate the relevance of the splice variants. The pedigree suggested a highly penetrant, autosomal dominant mode of inheritance. The phenotype of the family was suggestive of laminopathy, but previous genetic testing using both Sanger and panel sequencing only yielded conflicting evidence for LMNA p.R644C (rs142000963), which was not fully segregated. By re-sequencing four additional affected family members, further non-coding LMNA variants could be detected: rs149339264, rs199686967, rs201379016, and rs794728589. To explore the roles of these variants, iPSC-CMs were generated. RNA sequencing showed the LMNA expression levels to be significantly lower in the iPSC-CMs of the LMNA variant carriers. We demonstrated a dysregulated sarcomeric structure and altered calcium homeostasis in the iPSC-CMs of the LMNA variant carriers. Using targeted nanopore long-read sequencing, we revealed the biological significance of the variant c.356+1G>A, which generates a novel 5′ splice site in exon 1 of the cardiac isomer of LMNA, causing a nonsense mRNA product with almost complete RNA decay and haploinsufficiency. Using novel molecular analysis and nanopore technology, we demonstrated the pathogenesis of the rs794728589 (c.356+1G>A) splice variant in LMNA. This study highlights the importance of precise diagnostics in the clinical management and workup of cardiomyopathies. 相似文献
999.
Max Schuller Monika Oberhuber Barbara Prietl Elmar Zügner Eva-Maria Prugger Christoph Magnes Alexander H. Kirsch Sabine Schmaldienst Thomas Pieber Marianne Brodmann Alexander R. Rosenkranz Philipp Eller Kathrin Eller 《International journal of molecular sciences》2022,23(22)
Coronavirus disease 2019 (COVID-19)-induced metabolic alterations have been proposed as a source for prognostic biomarkers and may harbor potential for therapeutic exploitation. However, the metabolic impact of COVID-19 in hemodialysis (HD), a setting of profound a priori alterations, remains unstudied. To evaluate potential COVID-19 biomarkers in end-stage kidney disease (CKD G5), we analyzed the plasma metabolites in different COVID-19 stages in patients with or without HD. We recruited 18 and 9 asymptomatic and mild, 11 and 11 moderate, 2 and 13 severely affected, and 10 and 6 uninfected HD and non-HD patients, respectively. Plasma samples were taken at the time of diagnosis and/or upon admission to the hospital and analyzed by targeted metabolomics and cytokine/chemokine profiling. Targeted metabolomics confirmed stage-dependent alterations of the metabolome in non-HD patients with COVID-19, which were less pronounced in HD patients. Elevated kynurenine levels and lipid dysregulation, shown by an increase in circulating free fatty acids and a decrease in lysophospholipids, could distinguish patients with moderate COVID-19 from non-infected individuals in both groups. Kynurenine and lipid alterations were also associated with ICAM-1 and IL-15 levels in HD and non-HD patients. Our findings support the kynurenine pathway and plasma lipids as universal biomarkers of moderate and severe COVID-19 independent of kidney function. 相似文献
1000.
Thomas Velten Frank Bauerfeld Herbert Schuck Sabine Scherbaum Christof Landesberger Karlheinz Bock 《Microsystem Technologies》2011,17(4):619-627
In this paper we present a new roll-to-roll embossing process allowing the replication of micro patterns with feature sizes
down to 0.5 μm. The embossing process can be run in ‘continuous mode’ as well as in ‘discontinuous mode’. Continuous hot embossing
is suitable for the continuous output of micro patterned structures. Discontinuous hot embossing has the advantage that it
is not accompanied by waste produced during the initial hot embossing phase. This is because in ‘discontinuous mode’, embossing
does not start before the foil has reached the target temperature. The foil rests between two parallel heating plates and
foil movement and embossing starts only after the part of the foil resting between the heating plates has reached a thermal
steady state. A new type of embossing master is used which is based on flexible silicon substrates. The embossing pattern
with sub-μm topographic resolution is prepared on silicon wafers by state of the art lithography and dry etching techniques.
The wafers are thinned down to a thickness of 40 μm, which guarantees the mechanical flexibility of the embossing masters.
Up to 20 individual chips with a size of 20 × 20 mm2 were assembled on a roller. Embossing experiments with COC foils showed
a good replication of the silicon master structures in the foil. The maximum depth of the embossed holes was about 70% of
the master height. 相似文献