全文获取类型
收费全文 | 1110篇 |
免费 | 85篇 |
国内免费 | 3篇 |
专业分类
电工技术 | 5篇 |
化学工业 | 464篇 |
金属工艺 | 12篇 |
机械仪表 | 18篇 |
建筑科学 | 38篇 |
矿业工程 | 1篇 |
能源动力 | 34篇 |
轻工业 | 130篇 |
水利工程 | 7篇 |
石油天然气 | 3篇 |
武器工业 | 1篇 |
无线电 | 57篇 |
一般工业技术 | 179篇 |
冶金工业 | 128篇 |
原子能技术 | 7篇 |
自动化技术 | 114篇 |
出版年
2023年 | 19篇 |
2022年 | 105篇 |
2021年 | 101篇 |
2020年 | 25篇 |
2019年 | 36篇 |
2018年 | 37篇 |
2017年 | 43篇 |
2016年 | 57篇 |
2015年 | 34篇 |
2014年 | 49篇 |
2013年 | 86篇 |
2012年 | 62篇 |
2011年 | 66篇 |
2010年 | 58篇 |
2009年 | 50篇 |
2008年 | 48篇 |
2007年 | 33篇 |
2006年 | 31篇 |
2005年 | 29篇 |
2004年 | 17篇 |
2003年 | 18篇 |
2002年 | 9篇 |
2001年 | 8篇 |
2000年 | 12篇 |
1999年 | 9篇 |
1998年 | 40篇 |
1997年 | 34篇 |
1996年 | 25篇 |
1995年 | 11篇 |
1994年 | 10篇 |
1993年 | 9篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 5篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1970年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有1198条查询结果,搜索用时 31 毫秒
31.
Dania Esposito Andrea Margonelli Emanuela Pace Maria Teresa Giardi Cecilia Faraloni Giuseppe Torzillo Alba Zanini 《Microgravity science and technology》2006,18(3-4):215-218
Photosynthetic microorganisms are expected to be useful to maintain an oxygenic atmosphere and to provide biomass for astronauts in the International Space Station as well as in future long-term space flights. However, fluxes of complex ionizing radiation of various intensities and energies make space an extreme environment for the microorganisms, affecting their photosynthetic efficiency. To automatically monitor the photosynthetic Photosystem II (PSII) activity of microorganisms under space conditions an optical biosensor, which utilizes chlorophyll fluorescence as biological transduction system, was built; the PSII activity was monitored by the biosensor during balloon flights at stratospheric altitudes of about 40 km. The effect of space stress on quantum yield of PSII varied among the tested species depending on the growth light conditions at which they were exposed during the flights. 相似文献
32.
In this paper, we propose a novel architecture for computer-aided engineering (CAE) of rectangular aperture arrays that takes advantage of semantic grid computing technologies. This allows the implementation of the CAE environment in a service-oriented framework, where CAE applications are built up at run time, by exploiting remote services through the grid. The identification and localization of remote services and their orchestration is simplified by semantic facilities, centered around a CAE ontology. This provides a structured, conceptual representation of hardware and software resources, including CAE services, which is understandable both by humans and software agents. The implemented prototype demonstrates how semantic grids satisfy the strong need for cooperation tools in the CAE of microwave circuits and antennas, and represents an effective pathway towards the automatic generation of design tools, attained by integrating electromagnetic software available through the Web. 相似文献
33.
34.
This paper presents a market-enabling framework where users, content providers and network operators can interact in the seamless, transparent sale and delivery of a wide range of services. The framework allows for dynamic creation, configuration and delivery of services with quality assurance, via automated management of Service Level Agreements (SLAs). We propose an approach relying on a systemic treatment of business, service and network layer issues, which translates into a layered architecture where components belonging to different levels interact on the basis of a mediation paradigm. We use mediation as a generic term for a combination of negotiation, brokerage and state notification, jointly concurring to the realization of the entire life-cycle of a service. 相似文献
35.
Alexandra C. Green Lena M. Lidfors Sabrina Lomax Livio Favaro Cameron E.F. Clark 《Journal of dairy science》2021,104(1):826-838
Mammalian vocalizations can encode contextual information in both the spectrographic components of their individual vocal units and in their temporal organization. Here we observed 23 Holstein-Friesian dairy cows immediately after birth during interactions with their calf and when their calf was separated to the other side of a fence line. We investigated whether the vocalizations emitted in these postpartum contexts would vary temporally. We also described the maternal and stress behaviors preceding and following postpartum vocal production using kinematic diagrams and characterized call sequence structure. The kinematic diagrams highlight the disruption of maternal responses caused by calf separation and show that behavioral and vocal patterns varied according to the cows' emotional states and proximity to the calf in both contexts. During calf interactions, cows mainly produced closed-mouth calls simultaneous to licking their calf, whereas an escalation of stress responses was observed during calf separation, with the cows approaching the fence line, becoming alert to the calf, and emitting more mixed and open-mouth calls. Call sequences were similarly structured across contexts, mostly containing repetitions of a single call type, with a mean interval of 0.57 s between calls and a greater cumulative vocalization duration, attributed to an increased number of vocal units per sequence. Overall, calf separation was associated with a greater proportion of calls emitted as a sequence (inverse of single isolated calls), a shorter interval between separate call sequences, and a greater number of vocal units per sequence, compared with calf interactions. These temporal vocal features varied predictably with the high stress expression from cows during calf separation and may represent temporal modulations of emotional expression. Despite the noisy farm soundscape, empirical call type and temporal vocal features were easy to measure; thus, findings could be applied to future cattle studies wishing to analyze vocalizations for on-farm welfare assessments. 相似文献
36.
Aniello Esposito Martin Frey Andreas Schenk 《Journal of Computational Electronics》2009,8(3-4):336-348
In this work, the particular and combined influence of nonparabolicity and phonon scattering on the device characteristics of a triple-gate silicon nanowire is investigated. In addition, different approximations of the retarded self-energy for electron-phonon scattering are analyzed in terms of the electrostatics, current and computational cost. 相似文献
37.
Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing 下载免费PDF全文
Chlapanidas Theodora Perteghella Sara Faragò Silvio Boschi Alessandra Tripodo Giuseppe Vigani Barbara Crivelli Barbara Renzi Sabrina Dotti Silvia Preda Stefania Marazzi Mario Torre Maria Luisa Ferrari Maura 《应用聚合物科学杂志》2016,133(5)
In this work platelet lysate (PL) and adipose‐derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico‐chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 42942. 相似文献
38.
Lauralie Mangeot-Peter Sylvain Legay Jean-Francois Hausman Sergio Esposito Gea Guerriero 《International journal of molecular sciences》2016,17(9)
Gene expression profiling via quantitative real-time PCR is a robust technique widely used in the life sciences to compare gene expression patterns in, e.g., different tissues, growth conditions, or after specific treatments. In the field of plant science, real-time PCR is the gold standard to study the dynamics of gene expression and is used to validate the results generated with high throughput techniques, e.g., RNA-Seq. An accurate relative quantification of gene expression relies on the identification of appropriate reference genes, that need to be determined for each experimental set-up used and plant tissue studied. Here, we identify suitable reference genes for expression profiling in stems of textile hemp (Cannabis sativa L.), whose tissues (isolated bast fibres and core) are characterized by remarkable differences in cell wall composition. We additionally validate the reference genes by analysing the expression of putative candidates involved in the non-oxidative phase of the pentose phosphate pathway and in the first step of the shikimate pathway. The goal is to describe the possible regulation pattern of some genes involved in the provision of the precursors needed for lignin biosynthesis in the different hemp stem tissues. The results here shown are useful to design future studies focused on gene expression analyses in hemp. 相似文献
39.
Roberta Gentilini Sabrina Bozzini Fabiola Munarin Paola Petrini Livia Visai Maria Cristina Tanzi 《应用聚合物科学杂志》2014,131(2)
Extraction, purification, and gel preparation of Aloe Vera pectin and the evaluation of the biocompatibility of the pectin gels were studied, considering as end use as implantable materials for regenerative medicine. A. Vera was chosen as source of pectin, as this pectin was described to possess high molecular weight and a low degree of esterification. As the properties of pectins are strictly dependent upon the extraction methods in combination with the natural source, the extraction method was modified in order to optimize the yield of the final product, its purity, the duration of the process and the selection of non‐toxic chemical reagents. Changing the experimental conditions resulted in four different extraction processes and products with different physical and chemical characteristics. The optimal extraction resulted to be the process: with enzimatic deactivation by microwave and the use of sodium citrate as chelating agent the molecular weight of the pectin extracted was estimated to be 118 kDa and the 2.93% esterification degree. Cytocompatibility of pectin gels, prepared by ionotropic gelation, showing an improved cell adhesion if compared to commercial pectin. The results suggest that the extracted A. Vera pectins possess interesting properties to be exploited for the production of mechanically stable gels by ionotropic gelation and high rhamnose content matrices for application in regenerative medicine. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 39760. 相似文献
40.
Mario Lhr Wolfgang Hrtig Almut Schulze Matthias Kroiß Silviu Sbiera Constantin Lapa Bianca Mages Sabrina Strobel Jennifer Elisabeth Hundt Simone Bohnert Stefan Kircher Sudha Janaki-Raman Camelia-Maria Monoranu 《International journal of molecular sciences》2022,23(7)
Targeting molecular alterations as an effective treatment for isocitrate dehydrogenase-wildtype glioblastoma (GBM) patients has not yet been established. Sterol-O-Acyl Transferase 1 (SOAT1), a key enzyme in the conversion of endoplasmic reticulum cholesterol to esters for storage in lipid droplets (LD), serves as a target for the orphan drug mitotane to treat adrenocortical carcinoma. Inhibition of SOAT1 also suppresses GBM growth. Here, we refined SOAT1-expression in GBM and IDH-mutant astrocytoma, CNS WHO grade 4 (HGA), and assessed the distribution of LD in these tumors. Twenty-seven GBM and three HGA specimens were evaluated by multiple GFAP, Iba1, IDH1 R132H, and SOAT1 immunofluorescence labeling as well as Oil Red O staining. To a small extent SOAT1 was expressed by tumor cells in both tumor entities. In contrast, strong expression was observed in glioma-associated macrophages. Triple immunofluorescence labeling revealed, for the first time, evidence for SOAT1 colocalization with Iba1 and IDH1 R132H, respectively. Furthermore, a notable difference in the amount of LD between GBM and HGA was observed. Therefore, SOAT1 suppression might be a therapeutic option to target GBM and HGA growth and invasiveness. In addition, the high expression in cells related to neuroinflammation could be beneficial for a concomitant suppression of protumoral microglia/macrophages. 相似文献