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81.
82.
A polymerase chain reaction (PCR)-based assay was developed for detection of insertion sequence 900 (IS900) of Mycobacterium avium subsp. paratuberculosis in raw milk. This IS900 PCR assay included DNA extraction and PCR assay using commercially available kits. The DNA extraction and PCR assay were optimized to detect the IS900 sequence directly from raw milk. The IS900 PCR assay was evaluated by inoculating raw bulk milk and Middlebrook's 7H9 broth with 0 to 10(8) cfu/ml of each of four American Type Culture Collection strains of M. paratuberculosis. Under experimental conditions, both milk culture on Herrold's egg yolk medium slants, and IS900 PCR could detect 10 to 100 cfu/ml of M. paratuberculosis. Detection of M. paratuberculosis by IS900 PCR was consistent (24/24 PCR assays) when about 100 cfu/ml were present, whereas detection was variable (12/24 PCR assays) at concentrations as low as 10 cfu/ml. Based on the findings of the experimental study, IS900 PCR was further evaluated with pooled quarter milk samples from 211 cows from five herds with known history of Johne's disease. Out of 211 animals examined, nine (4%) and 69 (33%) were positive for M. paratuberculosis by milk culture and IS900 PCR from milk, respectively. A total of 20 bulk tank milk sample aliquots (one sample, four aliquots from each herd) were also examined, of which 10 (50%) were positive for M. paratuberculosis by IS900 PCR. By contrast, only one out of 20 (5%) bulk tank milk sample aliquots was positive by culture. The IS900 PCR amplified product of 229-bp obtained on testing of quarter milk and bulk tank milk samples was confirmed to be the IS900 of M. paratuberculosis by DNA sequence analysis. The results of this study suggest that M. paratuberculosis can be detected directly from quarter milk and bulk tank milk by IS900 PCR. 相似文献
83.
Prof. Dr. Hans Ulrich Buhl Dr. Bernd Heinrich Prof. Dr. Peter Loos Prof. Dr. Ulrich Frank Visiting Prof. Daniel L. Moody PhD Prof. Jeffrey Parsons PhD Prof. Dr. Michael Rosemann Prof. Dr. Elmar J. Sinz Prof. Ron Weber PhD Achim Kindler Dr. Prof. Dr. Claus Rautenstrauch Dipl.-Wirt.-Inf. Peter Fettke 《WIRTSCHAFTSINFORMATIK》2005,47(2):152-161
84.
E. Rogers P. M. Zaris J. Wood H. Pillai 《Multidimensional Systems and Signal Processing》2006,17(2-3):107-118
In this paper the behavorial approach is applied to discrete linear repetitive processes, which are class of 2D systems of
both systems theoretic and applications interest. The main results are on poles and zeros for these processes, which have
exponential trajectory interpretations.
Published online: April 2006 相似文献
85.
HU Guoming WAN Hui ZHANG Youlin BAO Wujun 《武汉理工大学学报(材料科学英文版)》2006,21(2):154-157
1Introduction Themechanicalbehaviorofcellularmaterialsde pendsontheirrelativedensity,cellgeometryandthema terialpropertiesofthesolidfromwhichtheyaremade.Theelasticmoduliofcellularmaterialscanbederived fromananalysisofthedeformationmechanismofthecell members.Thesimplegeometryofanidealizedunitcell allowsthederivationofrelationsbetweenmicro structural featuresandeffectivemechanicalproperties.Manytheoreticalmodelshavebeendevelopedtopre dicttheelasticmoduliofcellularmaterials[14].AbdEl Sayedetal[… 相似文献
86.
A. Redfearn BSc. PhD. M. May. BSc MSc D. Roberts BSc PhD 《Water and Environment Journal》2004,18(3):139-143
The paper presents an ecological risk-assessment methodology which (a) addresses surface-water exposures to aquatic mammals and birds, and (b) accounts for long-term accumulation and biomagnification of pollutants. The methodology has been adapted from those developed in the USA for compliance with US Environmental Protection Agency requirements, and combines predicted or measured surface-water concentration data with information on the natural history of wildlife species and chemical-specific bioaccumulation factors, to determine whether estimated exposures would exceed toxicological criteria. Whilst the methodology is applied to metals in leachate discharges from landfill sites, the general approach is applicable to other industrial sites. The approach could provide the basis for a shift towards more numerical risk-based ecological assessments in a wide variety of contexts. 相似文献
87.
88.
An interesting mechanical property of SiC/Al composites is that the tensile yield stress is less than the compressive yield
stress, even though the apparent modulus in tension is greater than that in compression. An investigation was undertaken to
determine if the Bauschinger effect (BE) in a SiC/Al composite is asymmetrical. It was found that the BE is indeed asymmetrical
in the case of the composite and the magnitude of the BE increases with total forward strain. These results can be explained
in terms of the changes in “back stress” caused by the changes in the residual stress and the work hardening during forward
strain. 相似文献
89.
We present a novel easy-to-align external cavity configuration for efficiently obtaining a near-diffraction limited single-lobed beam from gain-guided laser diode arrays. The external cavity has an external mirror (EM) in the image plane of the array. Two bulk-optics experimental setups are evaluated and a compact GRIN lens design is discussed. There are several advantages for the present geometry, including orders of magnitude lower misalignment-sensitivity and superior mode discrimination. Both experimental setups deliver near-diffraction limited single-lobed beams (of width ~0.8° at the rated output power of 100 mW of the array) in the entire current range. The light-current (L-I) characteristic is linear and kink-free and one of the setups shows higher efficiency than the free-running array. The broad-area laser (BAL) mode theory is discussed, introducing radiation losses to the analysis. Several aspects of amplification in laser arrays are theoretically explained, such as why the optimum angle of the far-field lobe is always greater than the tenth mode angle (for a uniform 10-stripe array). It is shown that the gain of the amplifying medium broadens the far-field profile. The width of the far-field lobe increases with gain and decreases with the lobe angle. An analytical expression for the broadened far-field profile of BAL modes is presented. The theory compares very well with the experiments 相似文献
90.
Affective computing 总被引:1,自引:0,他引:1
C. L. Lisetti PhD 《Pattern Analysis & Applications》1998,1(1):71-73