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41.
High Precision,Electrochemical Detection of Reversible Binding of Recombinant Proteins on Wide Bandgap GaN Electrodes Functionalized with Biomembrane Models 下载免费PDF全文
Nataliya Frenkel Jens Wallys Sara Lippert Jörg Teubert Stefan Kaufmann Aparna Das Eva Monroy Martin Eickhoff Motomu Tanaka 《Advanced functional materials》2014,24(31):4927-4934
We report a novel hybrid charge sensor realized by the deposition of phospholipid monolayers on highly doped n‐GaN electrodes. To detect the binding of recombinant proteins with histidine‐tags, lipid vesicles containing chelator lipids were deposited on GaN electrodes pre‐coated with octadecyltrimethoxysilane monolayers. Owing to its optical transparency, GaN allows the confirmation of the fluidity of supported membranes by fluorescence recovery after photo‐bleaching (FRAP). The electrolyte‐(organic) insulator‐semiconductor (EIS) setup enables one to transduce variations in the surface charge density ΔQ into a change in the interface capacitance ΔC p and, thus, the flat‐band potential ΔU FB. The obtained results demonstrate that the membrane‐based charge sensor can reach a high sensitivity to detect reversible changes in the surface charge density on the membranes by the formation of chelator complexes, docking of eGFP with histidine tags, and cancellation by EDTA. The achievable resolution of ΔQ ≥ 0.1 μC/cm2 is better than that obtained for membrane‐functionalized p‐GaAs, 0.9 μC/cm2, and for ITO coated with a polymer supported lipid monolayer, 2.2 μC/cm2. Moreover, we examined the potential application of optically active InGaN/GaN quantum dot structures, for the detection of changes in the surface potential from the photoluminescence signals measured at room temperature. 相似文献
42.
Sourabh Ghosh Sara T. Parker Xianyan Wang David L. Kaplan Jennifer A. Lewis 《Advanced functional materials》2008,18(13):1883-1889
Three–dimensional, microperiodic scaffolds of regenerated silk fibroin have been fabricated for tissue engineering by direct ink writing. The ink, which consisted of silk fibroin solution from the Bombyx mori silkworm, was deposited in a layer‐by‐layer fashion through a fine nozzle to produce a 3D array of silk fibers of diameter 5 µm. The extruded fibers crystallized when deposited into a methanol‐rich reservoir, retaining a pore structure necessary for media transport. The rheological properties of the silk fibroin solutions were investigated and the crystallized silk fibers were characterized for structure and mechanical properties by infrared spectroscopy and nanoindentation, respectively. The scaffolds supported human bone marrow‐derived mesenchymal stem cell (hMSC) adhesion, and growth. Cells cultured under chondrogenic conditions on these scaffolds supported enhanced chondrogenic differentiation based on increased glucosaminoglycan production compared to standard pellet culture. Our results suggest that 3D silk fibroin scaffolds may find potential application as tissue engineering constructs due to the precise control of their scaffold architecture and their biocompatibility. 相似文献
43.
To reduce the number of radio frequency (RF) chains in multiple input multiple output (MIMO) systems, generalized spatial modulation (GSM) techniques have been proposed in the literature. In this paper, we propose a zero‐forcing (ZF)‐based detector, which performs an initial pruning of the search tree that will be considered as the initial condition in a sphere decoding (SD) algorithm. The proposed method significantly reduces the computational complexity of GSM systems while achieving a near maximum likelihood (ML) performance. We analyze the performance of the proposed method and provide an analytic performance difference between the proposed method and the ML detector. Simulation results show that the performance of the proposed method is very close to that of the ML detector, while achieving a significant computational complexity reduction in comparison with the conventional SD method, in terms of the number of visited nodes. We also present some simulations to assess the accuracy of our theoretical results. 相似文献
44.
A double-gate (DG) fin field effect transistor (FinFET) is discussed as new label-free ion and biological sensor. Simulations as function of channel doping, geometrical dimensions, operation point and materials investigated the device response to an external potential difference which provides a body threshold voltage modulation. The simulation results presented in this work clearly state the key features for an ultrasensitive FET based sensor: an enhancement low doped and partially gated transistor operating in weak-moderate inversion regime. The optimized sensitivity, obtained when the width of the fin is equal to the gate height (wNW ∼ hg), reaches a value of 85% for an extraction current, Id, of 0.1 μA. These results pave the way for the fabrication process of an innovative CMOS compatible sensing system. 相似文献
45.
João Serra Sara Sequeira Ismael Domingos Ana Paracana Ermelinda Maçôas Luís V. Melo Bernardo J. Pires Susana Cardoso Diana C. Leitao Helena Alves 《Advanced functional materials》2021,31(47):2105638
Light detection technologies are of interest due to their applications in energy conversion and optical communications. Single-crystal organic semiconductors, such as rubrene, present high detectivities and charge carrier mobility, making them attractive for light-sensing applications. Growth of high crystallinity organic crystals is achieved using vapor processes, forming crystals of arbitrary shapes and orientations and requiring posterior patterning processes. However, patterning the organic semiconductors using industry-standard microfabrication techniques is not straightforward, as these often cause irreversible damage to the crystals. Here the fabrication of patterned micrometric rubrene photosensors is demonstrated through a combination of photolithography and Reactive Ion Etching steps. Protective layers during microfabrication minimize degradation of optoelectronic properties of the organic single crystals during fabrication. Crystals undergoing the patterning process presented a survival rate of 39%. Photoresponse values of up to 41 mA W−1 are obtained under illumination at 500 nm. This opens a route for the industrial-scale fabrication process of high-performance optoelectronic devices based on organic crystals semiconductors. 相似文献
46.
With the increasing demands for mobile wireless sensor networks in recent years, designing an energy‐efficient clustering and routing protocol has become very important. This paper provides an analytical model to evaluate the power consumption of a mobile sensor node. Based on this, a clustering algorithm is designed to optimize the energy efficiency during cluster head formation. A genetic algorithm technique is employed to find the near‐optimal threshold for residual energy below which a node has to give up its role of being the cluster head. This clustering algorithm along with a hybrid routing concept is applied as the near‐optimal energy‐efficient routing technique to increase the overall efficiency of the network. Compared to the mobile low energy adaptive clustering hierarchy protocol, the simulation studies reveal that the energy‐efficient routing technique produces a longer network lifetime and achieves better energy efficiency. 相似文献
47.
Francesco Merola Simonetta Grilli Sara Coppola Veronica Vespini Sergio De Nicola Pasquale Maddalena Cosimo Carfagna Pietro Ferraro 《Advanced functional materials》2012,22(15):3267-3272
Investigation on the behavior of nematic liquid crystals on functionalized polar dielectric crystal substrates is accomplished. Very interesting effects can be observed in maneuvering liquid crystal droplets on the substrate surface, driven by electric fields generated by pyroelectric effect. Reversible drops fragmentation and self‐assembling in different configurations can be achieved. The dynamics of the observed phenomena is studied and the repeatability of the process is full assessed. 相似文献
48.
Scientometrics - There has been a generalization in the use of two publication practices by scientific journals during the past decade: (1) ‘article in press’ or early view, which... 相似文献
49.
High Salinity Relaying to Reduce Vibrio parahaemolyticus and Vibrio vulnificus in Chesapeake Bay Oysters (Crassostrea virginica) 下载免费PDF全文
Salina Parveen Michael Jahncke Sara Elmahdi Helen Crocker John Bowers Chanelle White Stephanie Gray Amanda C. Morris Kathy Brohawn 《Journal of food science》2017,82(2):484-491
Cases of Vibrio infections in the United States have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (Crassostrea virginica). Information is needed on how to reduce numbers of Vibrio parahaemolyticus and Vibrio vulnificus in bi‐valve molluscan shellfish (for example, oysters). The purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (RASs) as methods to reduce the abundance of V. parahaemolyticus and V. vulnificus in oysters. For relaying field trials, oysters were collected from approved harvest waters, temperature abused outside under a tarp for 4 h, and then transferred to high (29 to 33 ppt.) and moderate (12 to 19 ppt.) salinities. For RAS treatment trial, oysters were transferred to 32 to 34 ppt. salinity at 15 °C. After 7, 14, 21, and in some instances 28 d, oysters were collected and analyzed for V. parahaemolyticus and V. vulnificus levels using multiplex real‐time PCR. Initial levels of V. parahaemolyticus and V. vulnificus ranged from 3.70 to 5.64 log10 MPN/g, and were reduced by 2 to 5 logs after 21 to 28 d in high salinity water (29 to 34 ppt.). Oyster mortalities averaged 4% or less, and did not exceed 7%. Relaying of oysters to high salinity field sites or transfer to high salinity RAS tanks was more effective in reducing V. vulnificus compared with V. parahaemolyticus. These results suggest that high salinity relaying of oysters is more effective in reducing V. vulnificus than V. parahaemolyticus in the oyster species used in this study. 相似文献
50.
Sara Sadiq Muhammad Imran Muhammad Nadeem Hassan Mazhar Iqbal Yusuf Zafar Fauzia Yusuf Hafeez 《LWT》2014
Antimicrobial behavior of lactic acid bacteria (LAB) has been explored since many years to assess their ability to produce bacteriocin, a natural preservative, to increase the shelf life of food. This study aims to characterize bacteriocin producing strains of lactic acid bacteria isolated from acidic to slightly acidic raw vegetables including tomato, bell pepper and green chili and to investigate their potential to inhibit food related bacteria. Among twenty nine LAB screened for antimicrobial activity, three exhibited antagonism against closely related bacterial isolates which was influenced by varying temperature and pH. They were identified up to strain level as Lactococcus lactis subsp. lactis TI-4, L. lactis subsp. lactis CE-2 and L. lactis subsp. lactis PI-2 based on 16S rRNA gene sequence. Their spectrum of inhibition was observed against food associated strains of Bacillus subtilis and Staphylococcus aureus. Moreover, L. lactis subsp. lactis PI-2 selected on the basis of higher antimicrobial activity was further evaluated for bacteriocin production which was detected as nisin A and nisin Z. These findings suggest the possible use of L. lactis strains of vegetable origin as protective cultures in slightly acidic as well as slightly alkaline food by the bio-preservative action of bacteriocins. 相似文献