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101.
Younis A Krifucks O Fleminger G Heller ED Gollop N Saran A Leitner G 《The Journal of dairy research》2005,72(2):188-194
The involvement of Staphylococcus aureus exosecretions in bovine udder infection (Younis et al. 2003) suggests that four different monomer protein bands appearing between 36 and 31 kDa, are associated with the severity of the cow's infection response. Three out of these four bands have been identified by means of protein sequencing. Band B, with a MW of 35 kDa was identified as Panton-Valentaine leucocidin LukF'-PV chain- Staph. aureus; band C, with a MW of 32 kDa was identified as leucocidin chain LukM precursor- Staph. aureus; and band D was found to be similar, but not identical, to phosphatidylinositol-specific phospholipase-C-X. Bands B and C were purified by gel filtration using FPLC. The ability of these proteins to induce udder inflammation in vivo, and proliferation response in vitro and cytokine secretion were tested for both the crude exosecretions and purified bands. Three cows were inoculated intracisternally, with three quarters receiving either 0.007-0.008 mg (as total proteins) of Staph. aureus FR2449/1 bacterial exosecretion, pooled fraction 39-41 (bands B and C), or culture broth medium. The fourth quarter was left free as a control. Quarters that received fraction 39-41 of Staph. aureus FR2449/1, exhibited induced inflammation, which was indicated by increased somatic cell count and enhanced NAGase activity that was significantly higher than that of the original Staph. aureus FR2449/1 bacterial exosecretion. Proliferation tests of bovine blood lymphocytes in vitro showed that the pooled fraction 39-41 stimulated bovine proliferation of mononuclear cells much more than the original Staph. aureus FR2449/1 bacterial exosecretion. Secretion of TNF-alpha, IL-1beta, IL-6 and IL-8 was in accordance with the contents of LukF'-PV and LukM precursor in the exosecretions. The results suggest that LukM/ LukF' induce inflammation into the udder by a mechanism similar to that of LPS or by a unique mechanism(s) which requires further investigation. 相似文献
102.
Some numerical results related to the critical behavior of inelastic structures subjected to dynamic step loading are given. The underlying theory of dynamic quasi-bifurcations is adapted to the analysis of elastic-plastic solids. Two examples, a 2D truss and a plane stress column-like structure, are discussed at length. 相似文献
103.
Effects of the Consumption of Milk Biofortified with Selenium,Vitamin E,and Different Fatty Acid Profile on Immune Response in the Elderly 下载免费PDF全文
Karina Pfrimer Eduardo Ferriolli Paula Lumy Takeuchi Márcia SV Salles Arlindo Saran‐Netto Marcus A Zanetti Luiz C Roma‐Junior Camila Bitu Moreno Braga Fernanda A Domenici Yara ML Valim Adriana BP Paschoalato Larissa F Marchi Ana ECS Azzolini Eduardo A Donadi Edson Martinez Helio Vannucchi 《Molecular nutrition & food research》2018,62(4)
104.