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71.
I Kohno H Iwasaki M Okutani Y Mochizuki S Sano Y Satoh T Ishihara H Ishii S Mukaiyama H Ijiri S Komori K Tamura 《Canadian Metallurgical Quarterly》1997,14(1):71-84
Cells of the central nervous system (CNS) normally do not express detectable levels of major histocompatibility complex (MHC) Class I antigens. However, MHC Class I expression can be induced after virus infection. We tested the hypothesis that virus-induced Class I expression is mediated by lymphocytes or cytokines using lymphocyte- and cytokine-deficient mice. We used Theiler's murine encephalomyelitis virus (TMEV), which induces CNS demyelination that maps genetically to the D region of MHC Class I and is associated with high levels of Class I products. TMEV infection of severe combined immunodeficiency (SCID) and recombination activation gene-1-deficient mice, which lack B and T lymphocytes, resulted in equivalent H-2D and H-2K expression in brain and spinal cord, according to analysis of the area and intensity of immunoperoxidase staining. Class I antigens were demonstrated as early as 6 hours after infection, and they were more widely distributed than viral RNA, indicating that expression was induced indirectly via a soluble factor. To determine whether cytokines induced the expression, we infected mice lacking receptors for interferon-alpha/beta (IFN-alpha/beta R (-/-)), interferon-gamma (IFN-gamma R(-/-)), and tumor necrosis factor-alpha (TNFRp55(-/-)). TMEV-infected IFN-gamma R(-/-) and TN-FRp55(-/-) mice expressed Class I antigens in the CNS, whereas IFN-alpha/beta R(-/-) mice did not, establishing that IFN-alpha/beta mediated the expression. In contrast to the equivalent expression in SCID mice, we observed greater area and higher intensity of H-2D versus H-2K antigens in infected SCID mice reconstituted with normal spleen cells. Collectively, the data indicate that after TMEV infection, early generalized MHC Class I expression is mediated by IFN-alpha/beta independently of lymphocytes, but the differential regulation of H-2D over H-2K may be controlled by B and/or T lymphocytes. 相似文献
72.
Tran Thi Ngoc Lan Rokuro Nishimura Yukihiro Satoh Masayuki Yokoi 《Corrosion Science》2005,47(4):1023-1038
The atmospheric corrosion of marble was evaluated in terms of SO2 concentration as air pollution and climatic factors such as rainfall, relative humidity, temperature and so on under the field exposure. Marble of calcite type (CaCO3) was exposed to outdoor atmospheric environment with and without a rain shelter at four test sites in the southern part of Vietnam for 3-month, 1- and 2-year periods from July 2001 to September 2003. The thickness loss of marble was investigated gravimetrically. X-ray diffraction and X-ray fluorescent methods were applied to study corrosion products on marble. The corrosion product of marble was only gypsum (CaSO4 · 2H2O) and was washed out by rain under the unsheltered exposure condition. It was found that the most substantial factors influencing the corrosion of marble were rainfall, SO2 concentration in the air and relative humidity. Based on the results obtained, we estimated the dose-response functions for the atmospheric corrosion of marble in the southern part of Vietnam. 相似文献
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75.
采用无偏振片反射式LCD及超低功耗驱动技术制造移动显示器件 总被引:1,自引:1,他引:0
Kiyoshi Minoura Yasushi Asaoka Eiji Satoh Takashi Satoh Ichiro Ihara Sayuri Fujiwara Akio Miyata Yasuhisa Itoh Seijiro Gyoten Noboru Matsuda Yasushi Kubota 代永平 《现代显示》2010,(7):5-9,35
我们通过对材料和制备条件的精细化处理,生产出一种在低刷新频率下无闪烁和图像迟滞现象的反射式显示器。该显示器的功耗非常低,且可在宽温度范围内工作,是未来移动显示器件的潜力平台。 相似文献
76.
77.
T Satoh Y Takahashi N Oshida A Shimizu H Shinoda M Watanabe T Samejima 《Canadian Metallurgical Quarterly》1999,38(5):1531-1536
Factors contributing to the thermostability of inorganic pyrophosphatase (PPase) were investigated by examining chimeric PPases from Escherichia coli and Thermus thermophilus (Tth). Two chimeric PPase genes, T1-135E (residues 1-135 from the N terminus are comprised of Tth PPase and residues 136-173 are derived from the C terminus of E. coli PPase) and T1-149E [residues 1-149 from the N terminus are from Tth PPase and the rest (150-175) are from E. coli PPase], were constructed by random chimeragenesis. After the genes were overexpressed in the E. coli BL21(DE3) strain and the expression products were purified, we compared the characteristics of these chimeric PPases with those of the parental PPases. We found that the two chimeras had higher activity than either parent PPase at the optimum temperature. We also examined thermal stability in terms of CD spectra, fluorescence spectra, and thermal changes in enzyme activity. The results revealed that the thermal stability of T1-149E is similar to that of Tth PPase, but T1-135E is much more stable. This suggests that the four residues that are different between T1-135E and T1-149E may be critical for thermostability between the two chimeras. By comparing the three-dimensional structures of Tth and E. coli PPases, we deduced that the following two factors may contribute to differences in thermostability. (1) Two residues (Thr138 and Ala141 in the Tth PPase and His140 and Asp143 in the E. coli PPase) in the vicinity of the trimer-trimer interface were different. (2) The Ala144-Lys145 loop in the Tth PPase was deleted in the E. coli PPase and also in the T1-135E chimera. Therefore, we conclude that T1-135E was thermostabilized by these two factors, and also, the Tth PPase moiety may contribute to the structural integrity of the chimeric enzymes. 相似文献
78.
Rhizobitoxine is synthesized by the legume symbiont Bradyrhizobium elkanii and the plant pathogen Burkholderia andropogonis. Rhizobitoxine competitively inhibited 1-aminocyclopropane-1-carboxylate (ACC) synthase bLE-ACS2 from the tomato, a key enzyme in the pathway of ethylene biosynthesis. Based on this inhibition of ACC synthase, we have developed a new assay for rhizobitoxine. 相似文献
79.
Toshiyuki Satoh 《International journal of control》2013,86(5):449-456
A new principle of control system design called matching was recently introduced by Zakian. The principle requires the designer to choose a controller which makes a system match its environment. Characterization of matched controllers by means of Youla parameterization is discussed in this paper. Sufficient conditions for matching are given in the form of inequality constraints on the norm of the free parameters in the parametrizations based on nominal matched controllers. 相似文献
80.
K Nakata H Gotoh J Watanabe T Uetake I Komuro K Yuasa S Watanabe R Ieki H Sakamaki H Akiyama S Kudoh M Naitoh H Satoh K Shimada 《Canadian Metallurgical Quarterly》1999,93(2):667-673
After allogeneic bone marrow transplantation (allo-BMT), recipient alveolar macrophages (AM) are gradually replaced by AM of the donor origin. An influx of mononuclear phagocytes of donor origin to the lung is responsible for the repopulation, but the detailed kinetics remain unclear. We therefore studied 24 BMT recipients who underwent bronchoalveolar lavage (BAL) from 24 to 83 days after BMT. AM cell number, size, morphology, proliferating ability, and genotype of AM were measured. Before day 50, the number and size of AM in BAL fluid were similar to those of normal nonsmokers. However, after day 50, the mean number of AM increased threefold and the mean cell size decreased due to the increase of small AM. These small cells are presumably of donor origin based on DNA fingerprinting analysis and based on fluorescence in situ hybridization for the Y chromosome in a sex-mismatched case. Immunohistochemistry and cell cycle analysis demonstrated that the increase in AM number coincided with a remarkable increase of AM expressing proliferating cell nuclear antigen, suggesting that small AM are proliferating. This is the first report representing that augmented proliferation of donor AM in situ may contribute to the reconstitution of AM population after BMT. 相似文献