Interaction of kunjin virus with octyl-D-glucoside extracted Vero cell plasma membrane

Initial experiments using whole cells have shown that there were specific and saturable interactions between kunjin (KUN) virus and receptor molecules on the Vero cell surfaces. Solubilisation of Vero cell plasma membranes with octyl-D-glucoside (OG) yielded an extract which also interacted specifically with KUN virus. This was proven using electron microscopy. When the virus-OG-extract complex was exposed onto Vero cell monolayers, no KUN virus was observed to enter into the whole cells. This would imply that there was virus-receptor interaction with the OG-extract leaving no free virus to attach to the whole cells. The attachment kinetics of KUN virus was studied further using the Scatchard analysis which indicated the involvement of more than one interactive macromolecule in the attachment event.     

Effect of oligosaccharides on rejection and reperfusion injury after lung transplantation

PURPOSE: We developed two models that are modifications of our original poly(2-hydroxyethyl methacrylate) (PHEMA) core-and-skirt keratoprosthesis. In these keratoprostheses, the mechanical strength of the skirt has been considerably increased with divinyl glycol (DVG) as a cross-linking agent during polymerization. In one (KPro I), methyl methacrylate (MMA) was added as comonomer to increase cell adhesion, and in the other (KPro II), HEMA was polymerized with DVG without comonomer. The aim of this study was to evaluate the process of healing and biocolonization and to ascertain whether KPro I demonstrates better ingrowth than the mechanically stronger KPro II, after implantation in rabbit eyes. METHODS: Ten rabbits were used for each model and studied at five predetermined end points up to 26 weeks. The device was implanted as a full-thickness keratoprosthesis covered with a conjunctival flap. RESULTS: Neither prosthesis demonstrated extrusion or retroprosthetic membrane formation. There was no significant difference between the two types of prosthesis with respect to tissue ingrowth and surrounding tissue melting. Histologically, inflammation was not severe, but calcification was seen in most specimens. Evidence of biodegradation of the prosthesis also was seen. CONCLUSION: In our original keratoprosthesis, fibrovascular invasion had occurred into the prosthetic skirt, but wound dehiscence and low mechanical strength resulted in an unfavorable outcome. In this series, the mechanical properties were improved, and KPro II was stronger than KPro I. Therefore KPro II would be the preferred polymer combination for surgical manipulation. However, biodegradation and calcification require further investigation into the degree and significance of these adverse reactions.     

Analysis of the thermal behaviour of a low cost,single-family,more sustainable house in Porto Alegre,Brazil

Efforts have been made in Brazil to improve the thermal performance of low-cost buildings. Since 1997 studies on low-cost housing have been developed by Núcleo Orientado para a Inovação da Edificação, the construction sector of the Engineering College of the Rio Grande do Sul University, Brazil. In 2000 a prototype of a low-cost house was built on the university campus. This prototype has undergone several evaluations in an attempt to assess its thermal and environmental performance, like: energy consumption, rainwater harvesting, use of sustainably managed wood for window frames and doors, besides a detailed accounting of overall costs. The thermal performance of the house was monitored, by recording outdoor and indoor air temperatures and relative humidity, over the period of whole year. A brief appraisal of such results, as well as the annual heating and cooling degree-hours are presented. Daily swings in outdoor are a common climatic event in Porto Alegre and the building response to daily temperature swings, greater than 10 K, and hot and cold spells are presented. The results show that the thermal performance of the prototype is fairly satisfactory, considering the limitations of a low-cost house, when artificial heating and cooling is not affordable.