Field Test to Restore Original Geochemical Conditions in a Flooded Mine Area-An Essential Milestone for the Complete Remediation of the Königstein Uranium Mine

Mine Water and the Environment - Uranium was extracted in the Königstein uranium mine by in-situ leaching with sulfuric acid until 1990. The originally anoxic conditions in the ore body became...     

Microbiological and physicochemical properties of farm bulk tank milk and antimicrobial resistance of its dominant bacteria

This study determined the antibiotic resistance of the dominant bacteria in the 85 farm BTMs according to the guidelines recommended by the epidemiological cutoff values in the EUCAST. In addition, some physicochemical and microbiological properties of farm BTMs were investigated. The milk samples were divided into two groups according to their SCC values. The milk samples with higher SCC than 400,000 cells mL⁻¹ were further examined bacteriologically, and the antibiotic resistance of isolates was determined. The average TAMB value was 6.34 log CFU/mL in farm BTM. It was found that high-SCC values did not affect other physicochemical properties of BTM samples, such as fat, protein and total solids, except for lactose content. Seventy-two strains were isolated from 45 bulk milk samples. The most prevalent bacteria were Enterococcus spp. (23.61%). The other isolates were Citrobacter spp. (12.5%), Staphylococcus spp. (12.51%), Serratia spp. (11.12%), Klebsiella spp. (9.72%), Bacillus spp. (9.72%), and Enterobacter spp. (8.33%). In antibiotic resistance analysis, 52.6% of Enterobacterales isolates showed cefoxitin resistance, and nine Enterobacterales isolates were determined as the presumptive ESBL producers. None of them was confirmed as ESBL producers. Moreover, MDR was detected in 83.3% of Enterobacter spp. isolates and all Bacillus spp. isolates. The over and inappropriate use of antibiotics in mastitis treatment may cause antibiotic-resistant microorganisms in milk. It was found that 52.7% of the isolated bacteria were MDR, which could pose a risk to public health and food safety, with the consumer's increasing interest in consuming raw milk.     

Monitoring food quality - effect of curcumin in the development of polyethylene/thermoplastic starch based smart packaging

Smart packaging relies on the one-to-one interaction of food with its packaging or its environment to monitor food quality and safety. Colorimetric pH indicators (synthetic, natural) working in a smart food packaging system are particularly striking when used with fresh foodstuffs such as fish and meat that perish quickly and require real-time freshness monitoring. In this study, curcumin (Cur) was used as a natural pH indicator to produce sustainable smart packaging material. Towards this objective, low-density polyethylene (LDPE) and thermoplastic starch (TPS) blend-based films containing Cur were prepared using a twin screw extrusion and hot-pressing processes. Besides, two different compositions of LDPE/TPS mixture (50/50 and 70/30) were used as the matrix. Thermal, mechanical, morphological properties, an affinity for water, and color change properties of LDPE/TPS/Cur films were investigated. They showed a significant color change from yellow to brown at pH: 10 at the end of the seventh day, especially in the 50 LDPE/50 TPS mixture. 50 LDPE/50 TPS mixture with 7% curcumin content gave the highest tensile strength of 8.03 Mpa. When the same mixture was used to monitor chicken meat spoilage at 25°C, meat samples have shown color changes from light yellow to light brown due to the increased content of total volatile basic amines. As a result, it has been suggested that 50 LDPE/50 TPS mixture containing 7% Cur can be used as a smart packaging material.