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61.
M Tomohiro Y Aida M Inomata Y Ito A Mizuno S Sakuma 《Canadian Metallurgical Quarterly》1997,41(3):121-129
The purpose of this study is to examine if microencapsulated PC12 cells may provide long term effects to the hemiparkinsonian rats. A modified technique was used to encapsulate PC12 cells into gelled microspheres. We found that the PC12 cells can survive in the modified microcapsules in vitro. Most of the PC12 cells formed cluster 3 weeks after incubation. The PC12 cell-loaded microcapsules were also examined in vitro. Adult Sprague-Dawley rats, anesthetized with chloral hydrate, were injected unilaterally with 6-hydroxydopamine into the medial forebrain bundle. The effectiveness of this lesion was tested by measuring apomorphine or methamphetamine-induced rotation one month after lesioning. The unilaterally lesioned rats were transplanted with microencapsulated PC12 cells. Results showed that apomorphine and methamphetamine-induced rotations were greatly suppressed after transplantation. One year after the grafting, the animals were anesthetized with urethane for the voltammetric study. Low dose of KCl was directly injected into the grafted striatum through pressure microejection. We found that KCl-induced DA release, as measured by voltammetric techniques, was regenerated in the striatum. The animals were later sacrificed for histological examination. We found that capsules were present in the lesioned striatum one year after grafting. Most of the capsules contained no PC12 cell. However, some capsules were filled entirely with PC12 cells. Taken together, our data suggested that PC12 cells can survive in the capsule in vitro and may provide long-term dopaminergic effects to the hemiparkinsonian rats. 相似文献
62.
Projections from each layer of the entorhinal cortex (EC) of the cat were traced to the dentate gyrus (DG), Ammon's horn (CA), prosubiculum (ProSb), subiculum (Sb), presubiculum (PreSb) and parasubiculum (ParaSb); the anterograde or retrograde labeling method was used after stereotaxic injection of wheat germ agglutinin-horseradish peroxidase, cholera toxin B subunit, or Phaseolus vulgaris leucoagglutinin. On the side ipsilateral to the tracer-injection, layer II of the EC projected most abundantly to the outer half of the molecular layer (ML) of the DG, less abundantly to the almost entire thickness of the stratum lacunosum-moleculare (SLM) of CA2-3, moderately to the almost entire thickness of the SLM of CA1, and less to the outer part of the ML of the ProSb and Sb. Layer III projected abundantly to the almost entire thickness of the SLM of CA1 and outer part of the ML of the ProSb and Sb, and sparsely to the SLM of CA2-3. Layer IV projected sparsely to the pyramidal cell layer of the ProSb and Sb; Layer IV of the medial part (toward the ParaSb) of the EC projected further to the ML of the DG. Layer VI projected sparsely to the outer part of the ML of the DG, almost entire thickness of the SLM of CA1-3, and outer part of the ML of the ProSb and Sb. More temporal parts of the hippocampal region received the projections from progressively more medial and more rostral parts of layers II and III, and from progressively more rostral parts of layers IV and VI. The ML of the PreSb and ParaSb received projections from all layers of the medial part of the ipsilateral EC. The SLM of CA1 and ML of the ProSb, Sb and ParaSb received projections from layer II and/or III of the contralateral medial entorhinal area. 相似文献
63.
Yukio Mizuno Yasuichi Mitsuyama Atsushi Minoda Masayuki Nagao Masamitsu Kosaki 《Electrical Engineering in Japan》1996,116(2):1-9
The authors have been developing extruded polymer insulated superconducting power cables. Dielectric loss in electrical insulation cannot be ignored in superconducting cables since conductor loss in the cables is minimal. Studies so far show that ethylene-propylene rubber (EPR) is suitable as an electrical insulating material in the extruded polymer insulated superconducting cable design because it demonstrates excellent mechanical and relatively good electrical qualities at a cryogenic temperature. Widely used EPR includes some kinds of additives; however, their effect on tan δ of EPR at cryogenic temperature remains unknown. The effect of additives such as crosslinking agent and fillers on tan δ of EPR was examined at temperatures of between 4.2 K and 300 K. Thickness dependence of tan δ was also measured using EPR films of different thickness and an extruded EPR insulated cable sample. The results show that additives increase tan δ of relatively thin films of EPR even in the cryogenic temperature region; however, they do not have an intolerable dielectric loss in comparison with conductor loss and heat inflow of the superconducting cable. The remedy to tan δ increase due to the polymer contraction relative to shielding wires has been established. 相似文献
64.
TM Mizuno H Bergen T Funabashi SP Kleopoulos YG Zhong WA Bauman CV Mobbs 《Canadian Metallurgical Quarterly》1996,93(8):3434-3438
Mutations in the obese (ob) gene lead to obesity. This gene has been recently cloned, but the factors regulating its expression have not been elucidated. To address the regulation of the ob gene with regard to body weight and nutritional factors, Northern blot analysis was used to assess ob mRNA in adipose tissue from mice [lean, obese due to diet, or genetically (yellow agouti) obese] under different nutritional conditions. ob mRNA was elevated in both forms of obesity, compared to lean controls, correlated with elevations in plasma insulin and body weight, but not plasma glucose. In lean C57BL/6J mice, but not in mice with diet-induced obesity, ob mRNA decreased after a 48-hr fast. Similarly, in lean C57BL/6J controls, but not in obese yellow mice, i.p. glucose injection significantly increased ob mRNA. For up to 30 min after glucose injection, ob mRNA in lean mice significantly correlated with plasma glucose, but not with plasma insulin. In a separate study with only lean mice, ob mRNA was inhibited >90% by fasting, and elevated approximately 2-fold 30 min after i.p. injection of either glucose or insulin. These results suggest that in lean animals glucose and insulin enhance ob gene expression. In contrast to our results in lean mice, in obese animals ob mRNA is elevated and relatively insensitive to nutritional state, possibly due to chronic exposure to elevated plasma insulin and/or glucose. 相似文献
65.
S. Nakagawa M. Kuno Y. Naito T. Nozawa T. Momma J. Mizuno T. Tsukamoto S. Motohashi M. Niwa 《Nuclear Engineering and Design》1998,179(2):145-156
Forced vibration tests were carried out at the Hamaoka (BWR) Unit 4 R/B (reactor building) in Japan in April and May of 1992. Fundamental dynamic characteristics of the R/B were obtained, including its interaction with the adjacent T/B (turbine building) and the soil–structure interaction. Results for the preceding R/Bs are compared, and probable causes for fluctuations in the resonance curve around the 1st peak are discussed. Furthermore, simulation analyses of the fundamental dynamic characteristics of the soil–structure system were conducted, using a basic lumped-mass soil–structure model (lattice model), and strong correlation with the measured data was obtained. Other detailed simulation models were employed to investigate the effects of simultaneously induced vertical response and response of the adjacent turbine building on the lateral response of the reactor building. 相似文献
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69.
Fluorescent molecular thermometers based on polymers showing a temperature-induced phase transition and labeled with polarity-sensitive fluorescent benzofurazans are the most sensitive known. Here we show a simple and effective method for modulating the sensitive temperature ranges of fluorescent molecular thermometers based on such temperature-responsive polymers. 4-N-(2-acryloyloxyethyl)-N-methylamino-7-N,N-dimethylaminosulfonyl-2,1,3-benzoxadiazole was adopted as a polarity-sensitive fluorescent benzofurazan, and nine copolymers of two kinds of acrylamide derivative (N-n-propylacrylamide, N-isopropylacrylamide, and/or N-isopropylmethacrylamide) with a small amount of DBD-AE were obtained. The fluorescence intensities of these copolymers in aqueous solution sharply increased with increasing temperature over a small range (6-7 degrees C). In contrast, these fluorescent molecular thermometers differed from one another in the sensitive temperature range (between 20 and 49 degrees C). Moreover, the sensitive temperature ranges were well related to the acrylamide ratios in feed. In addition, the responses from these fluorescent molecular thermometers to the change in temperature were reversible and exactly repeatable during 10 cycles of heating and cooling (relative standard deviation of the fluorescence intensity, 0.44-1.0%). 相似文献
70.
Takeshi?TateyamaEmail author Seiichi?Kawata Toshiki?Oguchi 《Artificial Life and Robotics》2004,7(4):193-197
We described a new preteaching method for re-inforcement learning using a self-organizing map (SOM). The purpose is to increase
the learning rate using a small amount of teaching data generated by a human expert. In our proposed method, the SOM is used
to generate the initial teaching data for the reinforcement learning agent from a small amount of teaching data. The reinforcement
learning function of the agent is initialized by using the teaching data generated by the SOM in order to increase the probability
of selecting the optimal actions it estimates. Because the agent can get high rewards from the start of reinforcement learning,
it is expected that the learning rate will increase. The results of a mobile robot simulation showed that the learning rate
had increased even though the human expert had showed only a small amount of teaching data.
This work was presented in part at the 7th International Symposium on Artificial Life and Robotics, Oita, Japan, January 16–18,
2002 相似文献