Multiple emulsion-based systems carrying insulin: development and characterization

An insulin delivery system based on liquid surfactant membranes has been developed. The formulation was based on a w/o/w emulsion where an organic membrane separated two aqueous phases and the internal aqueous phase contained insulin. Sesame and cotton seed oils were used as organic membranes. In order to facilitate the transportation of glucose across the organic membrane various additives such as calcium stearate, lecithin, cholesterol, hexamine, stearic acid and glyceryl tristearate were used. The additives were found to be successful carriers for the transportation of glucose to the internal aqueous phase. Similarly, viscosity enhancers, e.g. cetostearyl alcohol, in the organic phase enhanced the immobilization of insulin. Various parameters affecting the stability of the emulsions were established. The developed system was characterized for insulin activity and insulin efflux profile.     

Hemostatic risk factors of coronary artery disease in the Chinese

Induction of the mitochondrial permeability transition in vitro is well-characterized and widely implicated in the mechanism of oxidant-induced cell death. Despite an abundance of in vitro evidence, implication of mitochondrial dysfunction in the mechanism of chemical toxicity in vivo awaits demonstration of the induction of the mitochondrial permeability transition in tissues from intoxicated animals. Menadione (2-methyl-1,4-naphthoquinone), an agent known to induce the permeability transition in isolated liver mitochondrial in vitro, was administered as a single bolus to adult male rats, and hepatic mitochondria were isolated 24 h later. Mitochondria from menadione-treated rats exhibited an increased sensitivity to calcium-induced inhibition of state 3 respiration and loss of respiratory control, as well as a greater sensitivity to calcium-induced calcium release that was inhibited by cyclosporine A. Associated with this was the depolarization of membrane potential and swelling of mitochondria from menadione-treated animals, but not control animals. Both the calcium-dependent depolarization and swelling of mitochondria from menadione-treated rats were inhibited by adding either cyclosporine A or ruthenium red. The results are consistent with the induction of the mitochondrial permeability transition and provide the first evidence for the manifestation of an increased sensitivity to this response as a result of chemical exposure in vivo.     

FAST

We propose Frontier Allocation Synchronized by Token passing (FAST), a distributed algorithm for online terrain coverage using multiple mobile robots, ensuring mutually exclusive selection of frontier cells. Many existing approaches cover the terrain in an irregular fashion, without considering the usability of the already covered region. For instance, in the task of floor cleaning in an office building, these approaches do not guarantee the cleanliness of large unbroken areas until a majority of the task is complete. FAST on the other hand, incrementally traverses the terrain generating structured trajectories for each robot. Following a structured trajectory for coverage path planning is proven to be a very powerful approach in literature. This renders large portions of the terrain usable even before the completion of the coverage task. The novel map representation techniques used in FAST render it scalable to large terrains, without affecting the volume of communication among robots. Moreover, the distributed nature of FAST allows incorporation of fault-tolerance mechanisms. Empirical investigations on maps of varied complexities and sizes both in simulation and on an experimental test-bed demonstrate that the proposed algorithm performs better than some of the benchmark approaches in terms of coverage completion time and less redundant coverage.