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221.
Combined column generation and constructive heuristic for a proportionate flexible flow shop scheduling 总被引:1,自引:1,他引:0
Yueh-Min Huang Der-Fang Shiau 《The International Journal of Advanced Manufacturing Technology》2008,38(7-8):691-704
In a proportionate flow shop problem, jobs have to be processed through a fixed sequence of machines, and processing time for each job is equal on all machines. Such a problem has seldom been tackled. Proportionate flexible flow shop (PFFS) scheduling problems combine the properties of proportionate flow shop scheduling problems and parallel machine scheduling problems. This study presents a combined approach based on column generation (CG) for a PFFS problem with the criterion to minimize the objective of the total weighted completion time (TWCT). Minimizing TWCT in a PFFS problem significantly differs from the parallel-identical-machine scheduling problem, an optimal schedule in which jobs on each machine are in the weighted shortest processing time (WSPT) order. This combined approach adopts a CG approach to effectively handle job assignments to machines, and a constructive heuristic to obtain an optimal sequence for a single machine. Experimental results show the effectiveness of the combined approach in obtaining excellent quality solutions in a reasonable time, especially for large-scale problems. 相似文献
222.
The toxicity and species of dried dressed fish fillets were investigated. Three of seven dried dressed fish fillets were found to be toxic, with their levels of toxicity ranging from 9 to 18 mouse units per g. The toxin preparation was further purified and identified as tetrodotoxin and anhydrotetrodotoxin by high-performance liquid chromatography and gas chromatography-mass spectroscopy. The species of these fillets could not be distinguished from Lagocephalus lunaris by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis or restriction site and sequence analysis of a polymerase chain reaction amplicon of the cytochrome b gene. These fish fillets were identified as L. lunaris on the basis of their protein patterns and gene characteristics. Furthermore, the toxic samples contained low levels of tetrodotoxin and its derivative. 相似文献
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224.
During development of CGP56901, a monoclonal antibody (MAb) specific for a unique epitope on human IgE, the protein A-purified IgG from one of the candidate production cell lines, showed an additional minor heavy chain (H-chain) band with a molecular weight slightly lower than that of the principal H-chain band on SDS-PAGE. The N-terminal amino acid sequence of this minor H-chain species indicated that at least the first 30 amino acids were identical to those of the antibody light-chain (L-chain) variable domain. More detailed studies using peptide mapping and amino acid sequencing analysis confirmed a crossover event between the V genes of the antibody. The position is between Arg108 of the L chain and Ala124 of the H chain. This crossover resulted in a variant H chain, which had 16 fewer amino acid residues than the normal CGP56901 H chain. These results show that peptide mapping is a useful "first-line" analytical tool in the characterization of the quality of the monoclonal antibody. 相似文献