Maxwell’s methodology is combined with the boundary element method (BEM) for evaluating the two-dimensional effective elastic properties of composite, porous, and microcracked isotropic materials with periodic or random structure. The approach is based on the idea that the effective properties of the material can be deduced from the effects that a cluster of fibers, pores, or cracks embedded in an infinite matrix has on the far-fields. The fibers, pores, or cracks can have arbitrary shapes, sizes, and elastic properties, provided that the overall behavior is isotropic, and their effects are assumed to be the same as those of an equivalent circular inhomogeneity. The key aspect of the approach is to precisely account for the interactions between all the constituents in the cluster that represent the material in question. This is done by using the complex-variables version of the BEM to solve the problem of a finite cluster of fibers, pores or cracks embedded an infinite isotropic, linearly elastic matrix. The effective properties of the material are evaluated by comparing the far-field solutions for the cluster with that of the equivalent inhomogeneity. It is shown that the model adequately captures the influence of the micro-structure of the material on its overall properties. 相似文献
Mexedrone, α-PVP and α-PHP are synthetic cathinones. They can be considered amphetamine-like substances with a stimulating effect. Actually, studies showing their impact on DNA are totally absent. Therefore, in order to fill this gap, aim of the present work was to evaluate their mutagenicity on TK6 cells. On the basis of cytotoxicity and cytostasis results, we selected the concentrations (35–100 µM) to be used in the further analysis. We used the micronucleus (MN) as indicator of genetic damage and analyzed the MNi frequency fold increase by flow cytometry. Mexedrone demonstrated its mutagenic potential contrary to the other two compounds; we then proceeded by repeating the analyzes in the presence of extrinsic metabolic activation in order to check if it was possible to totally exclude the mutagenic capacity for α-PVP and α-PHP. The results demonstrated instead the mutagenicity of their metabolites. We then evaluated reactive oxygen species (ROS) induction as a possible mechanism at the basis of the highlighted effects but the results did not show a statistically significant increase in ROS levels for any of the tested substances. Anyway, our outcomes emphasize the importance of mutagenicity evaluation for a complete assessment of the risk associated with synthetic cathinones exposure. 相似文献
A new synthetic route to functionalized single walled carbon nanotubes (SWNTs) via supramolecular interactions using a specifically designed naphthalenediimide (NDI) nanoreceptor is demonstrated. The tendency of the NDI to spontaneously form composites with carbon nanomaterials leads to fluorescent amino acid tagged SWNTs, which are dispersible in widely accessible organic solvents (CHCl3, DMSO) as well as in biocompatible cell medium (EMEM, Eagle's modified essential medium). The X‐ray crystal structure of the first iodine‐tagged and amino acid‐functionalized NDI molecule, designed especially to facilitate the high resolution transmission electron microscopy (HR TEM) imaging whilst retaining its ability to self‐assemble into a nanodimensional receptor in weakly polar solvents, is also described. A new hybrid material, NDI@SWNT, was prepared and characterized as dispersed in organic solvents and aqueous media and in the solid state by HR TEM, tapping mode atomic force microscopy (TM AFM), scanning electron microscopy (SEM), circular dichroism, Raman and fluorescence spectroscopies (steady‐state single and two‐photon techniques). Combined microscopy techniques, density functional theory (DFT) calculations using the Spanish Initiative for Electronic Simulations with Thousands of Atoms (SIESTA) program and spectroscopic measurements in solution indicate that amino acid‐functionalized NDI interacts strongly with SWNTs and forms a donor‐acceptor complex. Density functional theory (DFT) calculations predicted the geometry and the binding energies of an NDI molecule loaded onto a SWNT strand and the possibility of charge transfer interactions within the hybrid. The NDI@SWNT composite translocates into cells (e.g. FEK‐4, HeLa, MCF‐7) as an intact object and localizes in the cells' cytoplasm and partially in the nucleus. The NDI coating enhances the biocompatibility of SWNTs and mediates its intracellular localization as shown by confocal fluorescence imaging and fluorescence lifetime imaging (FLIM) techniques. The excited state fluorescence lifetime of the probes in cells versus solution phase indicates that the probes remain unaffected by the change in their chemical environment within the experimental timescale (2 h). 相似文献
Résumé Dans le présent travail nous étudions la réaction d'oxydo-réduction de l'aluminium dans l'eutectique fondu NaCl–KCl–LiCl à 723 K en se penchant plus particulièrement sur le problème de la détermination de la valeur du nombre,n, d'électrons échangés au cours de cette réaction. Deux types d'expériences sont réalisées, tout d'abord des déterminations de l'activité des ions aluminium par mesure de fém, puis des mesures de cinétique à l'aide de la méthode chronopotentiométrique sur une électrode d'argent. Nous avons constaté que l'analyse des courbes provenant de ces méthodes nécessite la connaissance des coefficients d'activité ainsi que la variation du coefficient de diffusion, en fonction de temps pour en déduire une valeur den. La prise en compte de ces paramètres au cours de l'analyse des chronopotentiogrammes obtenus, conduit à des valeurs den voisines de 3 conformément à la réaction d'oxydo-réduction de l'aluminium.
A study of the redox reaction of aluminium in a NaCl–KCl–LiCl melt at 723 K has been carried out with particular emphasis on the problem of determination of the number of electrons,n, exchanged during this reaction. Two types of experiments were performed, initially the determination of the aluminium ion activity by e.m.f. measurement and, secondly, investigation of the reaction kinetics using chronopotentiometry on a silver electrode. For analysis of the curves resulting from this method, a knowledge of the activity coefficients and of the variation of the diffusion coefficient with time is needed in order to derive a value forn. Taking into account these parameters, a value ofn approximately equal to 3 was obtained for the aluminium redox reaction.
Data collection within a real-world environment may be compromised by several factors such as data-logger malfunctions and communication errors, during which no data is collected. As a consequence, appropriate tools are required to handle the missing values when analysing and processing such data. This problem is often tackled via matrix decomposition. While it has been successfully applied in a wide range of applications, in this work we report an issue that has been neglected in literature and “degenerates” the quality of the imputations obtained by matrix decomposition in multivariate time-series (with smooth evolution). Briefly, the problem consists of the misalignment of the matrix decomposition result: the missing values imputations fall within an incorrect range of values and the transitions between observed and imputed values are not smooth. We address this problem by proposing a post-processing alignment strategy. According to our experiments, the post-processing adjustment substantially improves the accuracy of the imputations (when the misalignment occurs). Moreover, the results also suggest that the misalignment occurs mostly when dealing with a small number of time-series due to lack of generalization ability.
Model-based testing overcomes challenges in software testing by generating automated test cases from behavior models, e.g. finite state machine (FSM) and input/output transition system (IOTS). Despite the existence of methods for IOTSs, the problem of selection of test cases is an important and difficult topic. The current methods from IOTSs do not provide the same support offered by the existing theory for FSMs, as complete fault coverage. In this paper, we propose a test generation method for IOTSs based on the W method developed for FSMs. The basic idea is to generate a transition cover set and a characterization set and concatenate them to generate complete test suites for IOTSs in a bounded number of steps. The method generates test suites with complete fault coverage for a given fault domain and is targeted at a class of IOTSs, called mealy IOTS, which accepts inputs only in stable states. Results from a case study show the proposed algorithm can achieve better results than a classical method for IOTSs. 相似文献