全文获取类型
收费全文 | 186篇 |
免费 | 5篇 |
专业分类
化学工业 | 58篇 |
金属工艺 | 2篇 |
机械仪表 | 3篇 |
建筑科学 | 7篇 |
矿业工程 | 1篇 |
能源动力 | 5篇 |
轻工业 | 57篇 |
水利工程 | 3篇 |
无线电 | 4篇 |
一般工业技术 | 28篇 |
冶金工业 | 9篇 |
自动化技术 | 14篇 |
出版年
2024年 | 1篇 |
2022年 | 7篇 |
2021年 | 10篇 |
2020年 | 5篇 |
2019年 | 11篇 |
2018年 | 6篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 12篇 |
2012年 | 17篇 |
2011年 | 18篇 |
2010年 | 11篇 |
2009年 | 15篇 |
2008年 | 7篇 |
2007年 | 10篇 |
2006年 | 7篇 |
2005年 | 7篇 |
2004年 | 4篇 |
2003年 | 7篇 |
2002年 | 2篇 |
2001年 | 2篇 |
1999年 | 1篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 2篇 |
排序方式: 共有191条查询结果,搜索用时 46 毫秒
101.
Carla Cofio Soledad Perez‐Amodio Carlos E. Semino Elisabeth Engel Miguel A. Mateos‐Timoneda 《大分子材料与工程》2019,304(11)
The introduction of 3D bioprinting to fabricate living constructs with tailored architecture has provided a new paradigm for biofabrication, with the potential to overcome several drawbacks of conventional scaffold‐based tissue regeneration strategies. Hydrogel‐based materials are suitable candidates regarding cell biocompatibility but often display poor mechanical properties. Self‐assembling peptides are a promising source of biomaterials to be used as 3D scaffolds based on their similarity to extracellular matrices (structurally and mechanically). In this study, an advanced bioink for biofabrication is presented based on the optimization of a RAD16‐I‐based biomaterial. The strategy followed to build 3D predefined structures by 3D printing is based on an enhancement of bioink viscosity by adding methylcellulose (MC) to a RAD16‐I solution. The resultant constructs display high shape fidelity and stability and embedded human mesenchymal stem cells present high viability after 7 days of culture. Moreover, cells are also able to differentiate to the adipogenic lineage, suggesting the suitability of this novel biomaterial for soft tissue engineering applications. 相似文献
102.
Igor Hernández Luis Javier R. Barrón Mailo Virto Francisco J. Pérez-Elortondo Cristian Flanagan Urko Rozas Ana Isabel Nájera Marta Albisu M. Soledad Vicente Mertxe de Renobales 《Food chemistry》2009
In this paper, we describe the effect of the addition of pregastric lipase on the composition and sensory properties of Idiazabal cheese. Free fatty acids (FFA), partial glycerides, free amino acids (FAA), gross composition and sensory characteristics were determined at different ripening times in cheeses manufactured with three different amounts of commercial animal lipase or with lipase-containing artisanal lamb rennet paste. The addition of lipase increased the content of total FFA, particularly of short-chain FFA, and that of total partial glycerides in cheeses. Unexpectedly, lipase utilization significantly affected total FAA concentration, which decreased in cheeses elaborated with high lipase amount. In general, Val, Glu and Leu were the major FAA, and their concentrations depended, mainly, on ripening time. Lipase addition had significant influence on the sensory characteristics of the cheeses, increasing scores for most of the flavour and odour attributes of the cheese. Principal component analysis (PCA) was done including dry matter, FFA, FAA, partial glycerides and odour and flavour attributes of the cheeses. It indicated that aroma and flavour parameters of Idiazabal cheese and the content of short-chain FFA and diglycerides were highly correlated to first principal component (PC1), while texture parameters, compositional variables and FAA were correlated to the second principal component (PC2). 相似文献
103.
María Elena López Rafael Barea Luis Miguel Bergasa María Soledad Escudero 《Journal of Intelligent and Robotic Systems》2004,40(3):233-265
One of the applications of service robots with a greater social impact is the assistance to elderly or disabled people. In these applications, assistant robots must robustly navigate in structured indoor environments such as hospitals, nursing homes or houses, heading from room to room to carry out different nursing or service tasks. Among the main requirements of these robotic aids, one that will determine its future commercial feasibility, is the easy installation of the robot in new working domains without long, tedious or complex configuration steps. This paper describes the navigation system of the assistant robot called SIRA, developed in the Electronics Department of the University of Alcalá, focusing on the learning module, specially designed to make the installation of the robot easier and faster in new environments. To cope with robustness and reliability requirements, the navigation system uses probabilistic reasoning (POMDPs) to globally localize the robot and to direct its goal-oriented actions. The proposed learning module fast learns the Markov model of a new environment by means of an exploration stage that takes advantage of human–robot interfaces (basically speech) and user–robot cooperation to accelerate model acquisition. The proposed learning method, based on a modification of the EM algorithm, is able to robustly explore new environments with a low number of corridor traversals, as shown in some experiments carried out with SIRA. 相似文献
104.
Rojo J Díaz V de la Fuente JM Segura I Barrientos AG Riese HH Bernad A Penadés S 《Chembiochem : a European journal of chemical biology》2004,5(3):291-297
Gold glyconanoparticles (GNPs) have been prepared as new multivalent tools that mimic glycosphingolipids on the cell surface. GNPs are highly soluble under physiological conditions, stable against enzymatic degradation and nontoxic. Thereby GNPs open up a novel promising multivalent platform for biological applications. It has recently been demonstrated that specific tumor-associated carbohydrate antigens (glycosphingolipids and glycoproteins) are involved in the initial step of tumor spreading. A mouse melanoma model was selected to test glyconanoparticles as possible inhibitors of experimental lung metastasis. A carbohydrate-carbohydrate interaction is proposed as the first recognition step for this process. Glyconanoparticles presenting lactose (lacto-GNPs) have been used successfully to significantly reduce the progression of experimental metastasis. This result shows for the first time a clear biological effect of lacto-GNPs, demonstrating the potential application of this glyconanotechnology in biological processes. 相似文献
105.
Magnetic nanoparticles have been used in therapeutic and diagnostic approaches in biomedicine for many years. For these applications, it is very important to investigate the nanoparticle-cell interactions. In this study we report a simple method for the preparation of gold-iron nanoparticles protected and functionalized with biologically relevant saccharides (maltose, lactose, and glucose). The nanoparticles were subsequently tested in vitro with a human fibroblast cell line to determine biocompatibility, and the cell-particle interactions, using fluorescence and scanning electron microscopies. Different cellular responses were obtained for each type of glyconanoparticle, demonstrating that the cells can recognize the saccharides on the nanoparticles. 相似文献
106.
Gene therapy, as therapeutic treatment to genetic or acquired diseases, is attracting much interest in the research community, leading to noteworthy developments over the past two decades. Although this field is still dominated by viral vectors, nonviral vectors have recently received an ever increasing attention in order to overcome the safety problems of their viral counterpart. This review presents the biological aspects involved in the gene delivery process and explores the recent developments and achievements of nonviral gene carriers. 相似文献
107.
Soledad Garcia Ferrari Harry Smith Edwar Calderon 《International Planning Studies》2018,23(4):355-375
After decades of internal conflict, Colombia is experiencing economic growth and urbanization. It remains, however, one of the most socially unequal countries in Latin America. Medellín, acclaimed the most innovative city, implemented large-scale transport infrastructures to link socially excluded areas to the city; new educational and cultural facilities; new public spaces and housing projects, rooted in the Barcelona model. This so-called ‘social urbanism’ has shifted local perceptions, though its socio-economic impact has been questioned. This paper focuses on the less analysed transformations in planning policy and management through two instruments: the Land Use Plan (Plan de Ordenamiento Territorial – POT) and the ‘Plan Parcial’. The research, based on a desktop review, interviews and site visits, examines the application of these instruments in Medellín, reflecting on how they contribute to achieving the aims of ‘social urbanism’. The paper explores the differences between ‘rhetoric’ and practice that are reflected in those between the city’s overarching plan (POT) and the implementation of ‘planes parciales’, focusing on redevelopment, urban renewal and urban expansion. Such differences mirror the deficiencies in the adaptation of the ‘urban project’ Barcelona model in Medellín, and provide the basis for a call to develop ‘social urbanism’ that is genuinely more socially, territorially comprehensive and inclusive. 相似文献
108.
109.
Irina Giralt Gabriel Gallo-Oller Natalia Navarro Patricia Zarzosa Guillem Pons Ainara Magdaleno Miguel F. Segura Constantino Sbado Raquel Hladun Diego Arango Jos Snchez de Toledo Lucas Moreno Soledad Gallego Josep Roma 《International journal of molecular sciences》2021,22(23)
The Wnt/β-catenin signaling pathway plays a pivotal role during embryogenesis and its deregulation is a key mechanism in the origin and progression of several tumors. Wnt antagonists have been described as key modulators of Wnt/β-catenin signaling in cancer, with Dickkopf-1 (DKK-1) being the most studied member of the DKK family. Although the therapeutic potential of DKK-1 inhibition has been evaluated in several diseases and malignancies, little is known in pediatric tumors. Only a few works have studied the genetic inhibition and function of DKK-1 in rhabdomyosarcoma. Here, for the first time, we report the analysis of the therapeutic potential of DKK-1 pharmaceutical inhibition in rhabdomyosarcoma, the most common soft tissue sarcoma in children. We performed DKK-1 inhibition via shRNA technology and via the chemical inhibitor WAY-2626211. Its inhibition led to β-catenin activation and the modulation of focal adhesion kinase (FAK), with positive effects on in vitro expression of myogenic markers and a reduction in proliferation and invasion. In addition, WAY-262611 was able to impair survival of tumor cells in vivo. Therefore, DKK-1 could constitute a molecular target, which could lead to novel therapeutic strategies in RMS, especially in those patients with high DKK-1 expression. 相似文献
110.
Cinta Zapater Ana Rocha Gregorio Mols Alessia Mascoli Soledad Ibaez Silvia Zanuy Ana Gmez 《International journal of molecular sciences》2021,22(18)
Although anti-Müllerian hormone (AMH) has classically been correlated with the regression of Müllerian ducts in male mammals, involvement of this growth factor in other reproductive processes only recently come to light. Teleost is the only gnathostomes that lack Müllerian ducts despite having amh orthologous genes. In adult teleost gonads, Amh exerts a role in the early stages of germ cell development in both males and females. Mechanisms involving the interaction of Amh with gonadotropin- and growth factor-induced functions have been proposed, but our overall knowledge regarding Amh function in fish gonads remains modest. In this study, we report on Amh actions in the European sea bass ovary. Amh and type 2 Amh receptor (Amhr2) are present in granulosa and theca cells of both early and late-vitellogenic follicles and cannot be detected in previtellogenic ovaries. Using the Pichia pastoris system a recombinant sea bass Amh has been produced that is endogenously processed to generate a 12–15 kDa bioactive mature protein. Contrary to previous evidence in lower vertebrates, in explants of previtellogenic sea bass ovaries, mature Amh has a synergistic effect on steroidogenesis induced by the follicle-stimulating hormone (Fsh), increasing E2 and cyp19a1a levels. 相似文献