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71.
Purification and characterization of a troponin C-like phosphodiesterase activator from bovine thyroid 总被引:1,自引:0,他引:1
A troponin C-like phosphodiesterase activator from bovine thyroid has been purified to homogeneity. The overall purification was about 9,800-fold with a yield of 8%. Bovine thyroid activator protein is identical in biologic properties to that isolated from bovine brain. They have the same specific activity regarding stimulation of bovine brain cyclic nucleotide phosphodiesterase. Both proteins form a Ca2+-dependent complex with heart muscle troponin I which is stable in 6M urea-polyacrylamide gel and which is similar, but not identical, to the troponin C-troponin I complex. The physiochemical properties of bovine thyroid activator protein are identical with those of bovine brain and other phosphodiesterase activator proteins and are similar to heart muscle and skeletal muscle troponin C as follows: (A) they bind 3-4 exchangeable calcium ions/mol with dissociation constants between 10(-5) and 10(-6) M, (B) they are highly acidic with a high content of aspartic and glutamic acids and isoelectric points of approximately 4.1, (C) these proteins have an unusual ultraviolet absorption spectrum with six discrete maxima between 250 and 284 nm which are characteristic of phenylalanine and tyrosine, and (D) these proteins have a low content of cysteine, histidine, tyrosine and proline. The tryptic peptide maps of bovine thyroid and brain activator protein are very similar. However, despite a very similar amino acid composition, the peptide map of bovine heart muscle troponin C is significantly different from that of the other two proteins. The molecular weight of thyroid and brain activator protein is 16,500, while that of heart troponin C is 18,500. Thyroid and brain activator protein, as well as heart troponin C, appear to undergo significant Ca2+-dependent conformational changes, as measured by the difference in the circular dichroism spectrum and electrophoretic mobility observed in the presence and absence of calcium ion. 相似文献
72.
Listeria monocytogenes is a common postprocess contaminant on ready-to-eat foods including premade ready-to-eat sandwiches. One popular type of sandwich product is the tortilla wrap, which contains sliced luncheon meats and cheeses rolled within a flour tortilla. This study determined the radiation resistance of L. monocytogenes surface inoculated onto two types of commercially available wheat flour tortillas, processed cheese slices, and deli turkey meat. The D10-values for L. monocytogenes (the radiation dose required to inactivate 1 log of the pathogen) were 0.27 kGy when inoculated onto two flour tortilla types, 0.28 and 0.30 kGy when inoculated onto two types of sliced processed cheeses, and 0.58 and 0.65 kGy when inoculated onto two types of sliced deli turkey meat. When two types of tortilla wraps were assembled from the individual components and L. monocytogenes was inoculated into the interfaces between the individual components, the D10-values were 0.27 to 0.37 kGy in the tortilla and cheese interfaces, 0.33 to 0.41 kGy in the cheese and turkey interfaces, and 0.25 to 0.33 kGy in the turkey and tortilla interfaces. The ability of ionizing radiation to reduce pathogen levels on the complex tortilla, cheese, and luncheon meat product was limited by the higher radiation resistance of L. monocytogenes when inoculated onto the ready-to-eat turkey-meat component. 相似文献
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IC Chi 《Canadian Metallurgical Quarterly》1996,12(2):77-99
Recent epidemiologic studies have suggested that tubal sterilization (TS) may lead to an increased incidence of subsequent hysterectomy but a decreased risk of ovarian cancer. This review evaluates the nature and magnitude of these two relationships, which should be of great concern and interest to women, clinicians and the administrators of family planning programs. The positive relationship between TS and subsequent hysterectomy is more likely to be of a motivational rather than biological nature, and a considerable number of unnecessary hysterectomies after TS could be avoided by changing the attitudes of physicians and women. The inverse relationship between TS and ovarian cancer appears causal, although the exact biological mechanisms remain to be clarified. Theoretically, this non-contraceptive beneficial effect of TS could be used as a primary preventive measure to curb the incidence of the highly fatal ovarian cancer. However, a number of medical, ethical, and economic questions attending use of a generally irreversible contraceptive procedure as a preventive measure must first be answered. The issue of whether TS is associated with any long-term sequelae, and, if so, whether the association is of a cause-and-effect nature or a by-product of time passage and aging of the woman, should be addressed by well-designed studies. 相似文献
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This paper examines the stability of geotextile-reinforced slopes when subjected to a vertical load applied to a strip footing positioned close to the slope crest. Vertical spacing between geotextile reinforcement was varied while maintaining a constant slope angle, load position, soil density and geotextile type. Small-scale physical tests were conducted using a large beam centrifuge to simulate field prototype conditions. After the model was accelerated to 40g, a load was applied to the strip footing until slope failure occurred. Digital image analysis was performed, using photographs taken in-flight, to obtain slope displacements and strain distribution along the reinforcement layers at different loading pressures during the test and at failure. Stability analysis was also conducted and compared with centrifuge model test results. The vertical spacing between reinforcement layers has a significant impact on the stability of a reinforced slope when subjected to a vertical load. Less vertical distance between reinforcement layers allows the slope to tolerate much greater loads than layers spaced further apart. Distributions of peak strains in reinforcement layers due to the strip footing placed on the surface of the reinforced slope were found to extend up to mid-height of the slope and thereafter they were found to be negligible. Stability analysis of the centrifuge models was found to be consistent with the observed performance of geotextile-reinforced slopes subjected to loading applied to a strip footing near the crest. 相似文献
80.
Alexander E.Braun 《集成电路应用》2008,(8):25-25
Charles Lieber博士领导哈佛大学的一个研究小组开发了一种约300nm厚的共轴硅纳米线,可以制作用来驱动小型电路和纳米机器的光伏(PV)电池。 相似文献