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郭惠玲 《武汉理工大学学报(材料科学英文版)》2018,33(3):559-565
A facile approach has been developed to synthesize Fe3O4@PAM (polyacrylamide) nanoparticles (NPs) with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe3O4 NPs aqueous suspension. Nitrilotriacetic acid (NTA) was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni2+ ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins (His-tagged GFP). The high magnetic property of Fe3O4@PAM@NTA-Ni2+ allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity (42 μg protein/mg of NPs). The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale. 相似文献
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刘苏 《西安建筑科技大学学报(自然科学版)》1990,(3)
本文是应用仿射对应原理,以椭圆曲线上4个点及其中心,求作椭圆的方法。以及用此方法解决某些一般形式的二次曲面中的一些几何作图问题。 相似文献
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高原冻土区地质条件复杂,冻土地基的差异性冻胀和融沉变形威胁着工程的长期稳定性和服役性能。为满足工程基础的工后沉降与沉降差要求,同时减少对下部冻土的扰动,以多年冻融区过渡段某富水场地为例,开展地基处理现场试验,对比块石抛填和块石抛填+换填两种地基处理施工方案。结果表明,针对包括淤泥质泥岩、粉砂质土、中粗砂、冻融区过渡带四种工程地质条件,采用块石抛填方案均出现翻浆、冒泥等现象,无法碾压达成地基处理要求,而采用块石抛填+换填处理方案,经8~10遍碾压后,压实度均能达到要求。 相似文献
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Suárez-Orozco Carola; Gaytán Francisco X.; Bang Hee Jin; Pakes Juliana; O'Connor Erin; Rhodes Jean 《Canadian Metallurgical Quarterly》2010,46(3):602
Immigration to the United States presents both challenges and opportunities that affect students' academic achievement. Using a 5-year longitudinal, mixed-methods approach, we identified varying academic trajectories of newcomer immigrant students from Central America, China, the Dominican Republic, Haiti, and Mexico. Latent class growth curve analysis revealed that although some newcomer students performed at high or improving levels over time, others showed diminishing performance. Multinomial logistic regressions identified significant group differences in academic trajectories, particularly between the high-achieving youth and the other groups. In keeping with ecological–developmental and stage–environment fit theories, School Characteristics (school segregation rate, school poverty rate, and student perceptions of school violence), Family Characteristics (maternal education, parental employment, and household structure), and Individual Characteristics (academic English proficiency, academic engagement, psychological symptoms, gender, and 2 age-related risk factors, number of school transitions and being overaged for grade placement) were associated with different trajectories of academic performance. A series of case studies triangulate many of the quantitative findings as well as illuminate patterns that were not detected in the quantitative data. Thus, the mixed-methods approach sheds light on the cumulative developmental challenges that immigrant students face as they adjust to their new educational settings. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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The distribution of neutrons produced by the reaction of 50MeV/u ^12C-ion on a thick Cu target are studied.The neutrons are measured with threshold activation detectors.Al.F,C,Al and In activation samples were used to measure neutrons with energy greater than 7,11,20,50MeV and thermal neutrons,respectively,The fluence rate,energy and angular distributions of neutrons,total neutron yield of 12C-ion and the emission rate in the forward direction of neutrons over 11 and 20MeV were obtained. 相似文献
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