Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior

Connexin43 (Cx43), the main gap junction and hemichannel forming protein in the urinary bladder, participates in the regulation of bladder motor and sensory functions and has been reported as an important modulator of day–night variations in functional bladder capacity. However, because Cx43 is expressed throughout the bladder, the actual role played by the detrusor and the urothelial Cx43 is still unknown. For this purpose, we generated urothelium-specific Cx43 knockout (uCx43KO) mice using Cre-LoxP system. We evaluated the day–night micturition pattern and the urothelial Cx43 hemichannel function of the uCx43KO mice by measuring luminal ATP release after bladder distention. In wild-type (WT) mice, distention-induced ATP release was elevated, and functional bladder capacity was decreased in the animals’ active phase (nighttime) when Cx43 expression was also high compared to levels measured in the sleep phase (daytime). These day–night differences in urothelial ATP release and functional bladder capacity were attenuated in uCx43KO mice that, in the active phase, displayed lower ATP release and higher functional bladder capacity than WT mice. These findings indicate that urothelial Cx43 mediated ATP signaling and coordination of urothelial activity are essential for proper perception and regulation of responses to bladder distension in the animals’ awake, active phase.     

A Blend of Ethanol and (−)-α-Pinene were Highly Attractive to Native Siricid Woodwasps (Siricidae,Siricinae) Infesting Conifers of the Sierra Nevada and the Allegheny Mountains

Woodwasps in Sirex and related genera are well-represented in North American conifer forests, but the chemical ecology of native woodwasps is limited to a few studies demonstrating their attraction to volatile host tree compounds, primarily monoterpene hydrocarbons and monoterpene alcohols. Thus, we systematically investigated woodwasp-host chemical interactions in California’s Sierra Nevada and West Virginia’s Allegheny Mountains. We first tested common conifer monoterpene hydrocarbons and found that (?)-α-pinene, (+)-3-carene, and (?)-β-pinene were the three most attractive compounds. Based on these results and those of earlier studies, we further tested three monoterpene hydrocarbons and four monoterpene alcohols along with ethanol in California: monoterpene hydrocarbons caught 72.3% of all woodwasps. Among monoterpene hydrocarbons, (+)-3-carene was the most attractive followed by (?)-β-pinene and (?)-α-pinene. Among alcohols, ethanol was the most attractive, catching 41.4% of woodwasps trapped. Subsequent tests were done with fewer selected compounds, including ethanol, 3-carene, and ethanol plus (?)-α-pinene in both Sierra Nevada and Allegheny Mountains. In both locations, ethanol plus (?)-α-pinene caught more woodwasps than other treatments. We discussed the implications of these results for understanding the chemical ecology of native woodwasps and invasive Sirex noctilio in North America. In California, 749 woodwasps were caught, representing five species: Sirex areolatus Cresson, Sirex behrensii Cresson, Sirex cyaneus Fabricius, Sirex longicauda Middlekauff, and Urocerus californicus Norton. In West Virginia 411 woodwasps were caught representing four species: Sirex edwardsii Brullé, Tremex columba Linnaeus, Sirex nigricornis F., and Urocerus cressoni Norton.     

Urban,suburban or rural? Understanding preferences for the residential environment

ABSTRACT

There are two ongoing trends that lead to changing preferences for the built environment. One concerns a demographic transition into more but smaller, and older, households. The other concerns greater possibilities to satisfy residential preferences due to rising incomes and technological advances. The current study explores the preference for the type of residential environment and the underlying motivations. The smaller municipality is most preferred (36%), followed by the city edge (32%), a rural area (13%), the city centre (11%) and no preference (7%). The city centre is preferred because of amenities, ambiance, liveliness and activities. The city edge is preferred because of peace and quiet and easy access to the dwelling. Social contact was rarely mentioned. In contrast, respondents who preferred a smaller municipality frequently mentioned social contact. Furthermore, feeling safe/secure and wellbeing were important items. Finally, respondents with a rural preference mentioned freedom and peace and quiet.     

Gestalt and phenomenal transparency

Phenomenal transparency is commonly studied by using a stimulus configuration introduced by Metelli: a bipartite patch, divided into equal left and right halves is overlaid with a smaller, concentric bipartite patch, divided along the same line. Observers are instructed to report either a transparent patch over an opaque bipartite field or a mosaic of four opaque patches. We show theoretically and empirically that these are only two of five generic perceptual categories, namely, transparent patch, transparent annulus (hole), mosaic, partial transparency, and multiple transparency (ambiguous) cases. Thus Gestalt factors complicate the interpretation "phenomenal transparency." We propose a framework that avoids this complication. There is excellent agreement between predictions and results.     

Comparison of the anthropometric and biochemical variables between children and their parents

The objective of this study was to compare the anthropometry, body composition and biochemical profile between eutrophic and obese children, as well as to relate the children's nutritional state and body composition to those of their respective parents. A control-case study was carried out with 50 eutrophic and 50 obese children paired according to gender, age, socioeconomic condition, and selected from the nutritional evaluation of 2 074 children aged 6 to 8 years, attending public and private urban schools in Vi?osa, Minas Gerais, Brazil. The measurements evaluated were weight, height, circumferences (waist and hip) and thickness skinfolds (triceps and subscapular). The serum levels of glucose, total-cholesterol, HDL, LDL, triglycerides and hemoglobin in the children were evaluated. The obese children presented a larger body size and higher percentage for total body fat as well as its accumulation in the central region, and higher serum levels for triglycerides (p < 0.05). For all biochemical parameters, except for hemoglobin and HDL, there occurred a positive correlation with the located fat in the central region as well as with the total body fat in children (p < 0.05). The obese mothers showing high percentage of body fat and its accumulation in the central region rather tend to have children with these characteristics (p < 0.05). The highest number of the close relatives who are obese and present dyslipidemia is highlighted in the obese children (p < 0.05). This study evidences the differences concerning to the distribution of the body fat and lipid profile among eutrophic and obese children, as well as the strong influence of the maternal obesity upon child's obesity.     

Effect of Dietary Cholesterol and Fat on Cell Cholesterol Transfer to Postprandial Plasma in Hyperlipidemic Men

Postprandial chylomicrons are potent ultimate acceptors of cell membrane cholesterol and are believed to accelerate reverse cholesterol transport (RCT). We compared the effects of meals rich in polyunsaturated fat (PUFA) and either high (605 mg) or low (151 mg) in cholesterol and a meal rich in dairy fat (DF) in the form of cream on net in vitro transport of red blood cell (RBC) membrane cholesterol to 4 and 6 h postprandial plasma in eight normotriglyceridemic (NTG-H) and eight hypertriglyceridemic (HTG-H) men with mild to moderate hypercholesterolemia. In HTG-H men, cell cholesterol accumulation in 6-h postprandial plasma was significantly (P = 0.02) less after the PUFA-HC meal compared with the other meals. The significant (P < 0.001) increase in cell plus endogenous cholesterol accumulation in the triglyceride-rich lipoprotein (TRL) fraction of 4 h postprandial plasma incubated with RBC was significantly (P = 0.007) higher after the PUFA-HC meal compared with DF meal in HTG-H men. In NTG-H men, cholesterol accumulation in plasma and plasma lipoproteins in the presence and absence of RBC was not significantly affected by the type of meal ingested. These data suggest that addition of large amounts of cholesterol to a PUFA meal may impair diffusion-mediated transport of cell membrane cholesterol to postprandial plasma and that replacing DF with PUFA in a meal increases postprandial lipemia and may potentially increase cholesterol accumulation in atherogenic postprandial TRL in HTG-H men.     

Development of Hydrophobic Starch

The aim of this work was a systematic investigation of the preparation conditions for stable aqueous dispersions of hydrophobic starch. Samples of benzylated starch were therefore synthesised and then converted into hydrophobic dispersions by fragmentation. In the study, the type of starch and process parameters such as the degree of hydrophobic substitution and fragmentation method were varied. It was shown that the type of derivatisation process (i.e. heterogeneous in a slurry or homogeneous in an autoclave) had a crucial influence on the dispersion properties. Dilution from homogeneous solution and steam‐jet cooking were explored as fragmentation methods for the starch derivatives. The resulting dispersions were comprehensively characterised in relation to the molecular properties of the soluble fraction and sediment. Significant differences in dispersion properties were caused by the type of starch used (i.e. potato or maize) and the derivatisation process, for a similar degree of benzylation.