Automation in the pharmaceutical analysis laboratory: a centralized/decentralized approach

It has been over 10 years since robots have appeared in the pharmaceutical analysis laboratory. In the early days, it was common for one selected individual to be responsible for the programming, usage and maintenance of the robots(s). However, the increasing use of robotics has prompted the formation of robotics ‘laboratories’ and/or ‘groups’. This is especially true when multiple robotic systems and applications are involved.Over the past several years at ISLAR, many champions of robotics have given presentations on the setup and usage of robotics within their organizations. These managers have described both the ‘centralized’ and ‘decentralized’ approaches to the implementation of robotics. In the centralized system, a single group is charged with all aspects of the robotic project, including justification, purchase, validation, use and maintenance. Under such an arrangement, samples are usually given to the robotics group for analysis. In contrast, a totally decentralized approach to robotics would have units interspersed throughout the organization, with each individual group responsible for their respective unit(s), in much the same way as liquid chromatographs are considered.At Hoffmann-La Roche, aspects of both the centralized and decentralized approaches to robotics are used which make our combined system the ‘best of both worlds’. This paper describes the Roche philosophy towards robotics and highlights the advantages to the system used.     

Enhancing self-scheduling algorithms via synchronization and weighting

Existing dynamic self-scheduling algorithms, used to schedule independent tasks on heterogeneous clusters, cannot handle tasks with dependencies because they lack the support for internode communication. To compensate for this deficiency we introduce a synchronization mechanism that provides inter-processor communication, thus, enabling self-scheduling algorithms to handle efficiently nested loops with dependencies. We also present a weighting mechanism that significantly improves the performance of dynamic self-scheduling algorithms. These algorithms divide the total number of tasks into chunks and assign them to processors. The weighting mechanism adapts the chunk sizes to the computing power and current run-queue state of the processors. The synchronization and weighting mechanisms are orthogonal, in the sense that they can simultaneously be applied to loops with dependencies. Thus, they broaden the application spectrum of dynamic self-scheduling algorithms and improve their performance. Extensive testing confirms the efficiency of the synchronization and weighting mechanisms and the significant improvement of the synchronized–weighted versions of the algorithms over the synchronized-only versions.     

<Emphasis Type="Italic">HumanPT</Emphasis>: An Open-source,HumanPT Architecture-based,Robotic Application for Low Cost Robotic Tasks

In this paper, first HumanPT architecture for low cost robotic applications is presented. HumanPT architecture differs than other architectures because it is implemented on existing robotic systems (robot  robotic controller) and exploits the minimum communication facilities for real-time control that these systems provide. It is based on well-known communication methods like serial communication (USB, RS232, IEEE-1394) and windows sockets (server–client model) and permits an important number of different type of components like actuators, sensors and particularly vision systems to be connected in a robotic system. The operating system (OS) used is Microsoft Windows, the most widely spread OS. The proposed architecture exploits features of this OS that is not a real-time one, to ensure – in case that the robotic system provide such a facility – control and real time communication with the robotic system controller and to integrate by means of sensors and actuators an important number of robotic tasks and procedures. As implementation of this architecture, HumanPT robotic application and experimental results concerning its performance and its implementation in real tasks are provided. HumanPT robotic application, developed in Visual C++, is an integrated, but simultaneously an open-source software that can be adapted in different types of robotic systems. An important number of robotic tasks or procedures including sensors and particularly vision systems can be generated and executed. Small enterprises by means of the proposed architecture and the open source software can be automated at low cost enhancing in this way their production.     

Evaluation Techniques for Generalized Path Pattern Queries on XML Data

Finding the occurrences of structural patterns in XML data is a key operation in XML query processing. Existing algorithms for this operation focus almost exclusively on path patterns or tree patterns. Current applications of XML require querying of data whose structure is complex or is not fully known to the user, or integrating XML data sources with different structures. These applications have motivated recently the introduction of query languages that allow a partial specification of path patterns in a query. In this paper, we consider partial path queries, a generalization of path pattern queries, and we focus on their efficient evaluation under the indexed streaming evaluation model. Our approach explicitly deals with repeated labels (that is, multiple occurrences of the same label in a query). We show that partial path queries can be represented as rooted dags for which a topological ordering of the nodes exists. We present three algorithms for the efficient evaluation of these queries. The first one exploits a structural summary of data to generate a set of path patterns that together are equivalent to a partial path query. To evaluate these path patterns, we extend a previous algorithm for path-pattern queries so that it can work on path patterns with repeated labels. The second one extracts a spanning tree from the query dag, uses a stack-based algorithm to find the matches of the root-to-leaf paths in the tree, and merge-joins the matches to compute the answer. Finally, the third one exploits multiple pointers of stack entries and a topological ordering of the query dag to apply a stack-based holistic technique. We analyze our algorithms and perform extensive experimental evaluations. Our experimental results show that the holistic algorithm outperforms the other ones. Our approaches are the first ones to efficiently evaluate this class of queries in the indexed streaming model.     

Molecular dynamics at the receptor level of immunodominant myelin basic protein epitope 87-99 implicated in multiple sclerosis and its antagonists altered peptide ligands: triggering of immune response

This work reports molecular dynamics studies at the receptor level of the immunodominant myelin basic protein (MBP) epitope 87-99 implicated in multiple sclerosis, and its antagonists altered peptide ligands (APLs), namely [Arg91, Ala96] MBP87-99 and [Ala91,96] MBP87-99. The interaction of each peptide ligand with the receptor human leukocyte antigen HLA-DR2b was studied, starting from X-ray structure with pdb code: 1ymm. This is the first such study of APL-HLA-DR2b complexes, and hence the first attempt to gain a better understanding of the molecular recognition mechanisms that underlie TCR antagonism by these APLs. The amino acids His88 and Phe89 serve as T-cell receptor (TCR) anchors in the formation of the trimolecular complex TCR-peptide-HLA-DR2b, where the TCR binds in a diagonal, off-centered mode to the peptide-HLA complex. The present findings indicate that these two amino acids have a different orientation in the APLs [Arg91, Ala96] MBP87-99 and [Ala91,96] MBP87-99: His88 and Phe89 remain buried in HLA grooves and are not available for interaction with the TCR. We propose that this different topology could provide a possible mechanism of action for TCR antagonism.     

Teachers' perceptions of the barriers to technology integration and practices with technology under situated professional development

This case study examines 18 elementary school teachers' perceptions of the barriers to technology integration (access, vision, professional development, time, and beliefs) and instructional practices with technology after two years of situated professional development. Months after transitioning from mentoring to teacher-led communities of practice, teachers continued to report positive perceptions of several barriers and were observed engaging in desirable instructional practices. Interviews suggest that the situated professional development activities helped create an environment that supported teachers' decisions to integrate technology. Implications for teacher professional development and technology integration are discussed in conjunction with the strengths and limitations of the study.     

Social class, child age, and parental socialization values.

Upper-, middle-, and lower-class parents were asked to explain a proverb which had both "growth" and "stability" interpretations to their 11- and 16-yr-old sons. The growth interpretation was overwhelmingly endorsed by most parents. With 16-yr-olds, lower-middle-class parents continued to endorse the growth interpretation; most upper-class parents endorsed the stability interpretation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Mental Health of Lesbian, Gay, Bisexual, and Heterosexual Siblings: Effects of Gender, Sexual Orientation, and Family.

Self-identified lesbian, gay male, and bisexual (LGB) individuals were recruited via convenience sampling, and they in turn recruited their siblings (79% heterosexual, 19% LGB). The resulting sample of 533 heterosexual, 558 lesbian or gay male, and 163 bisexual participants was compared on mental health variables and their use of mental health services. Multilevel modeling analyses revealed that sexual orientation predicted suicidal ideation, suicide attempts, self-injurious behavior, use of psychotherapy, and use of psychiatric medications over and above the effects of family adjustment. Sexual orientation was unrelated to current psychological distress, psychiatric hospitalizations, and self-esteem. This is the 1st study to model family effects on the mental health of LGB participants and their siblings. (PsycINFO Database Record (c) 2010 APA, all rights reserved)