全文获取类型
收费全文 | 39442篇 |
免费 | 4062篇 |
国内免费 | 2014篇 |
专业分类
电工技术 | 2377篇 |
技术理论 | 3篇 |
综合类 | 2673篇 |
化学工业 | 6741篇 |
金属工艺 | 2133篇 |
机械仪表 | 2384篇 |
建筑科学 | 3006篇 |
矿业工程 | 1264篇 |
能源动力 | 1159篇 |
轻工业 | 3876篇 |
水利工程 | 847篇 |
石油天然气 | 1907篇 |
武器工业 | 296篇 |
无线电 | 4529篇 |
一般工业技术 | 4779篇 |
冶金工业 | 1900篇 |
原子能技术 | 400篇 |
自动化技术 | 5244篇 |
出版年
2024年 | 201篇 |
2023年 | 779篇 |
2022年 | 1352篇 |
2021年 | 2055篇 |
2020年 | 1557篇 |
2019年 | 1257篇 |
2018年 | 1362篇 |
2017年 | 1521篇 |
2016年 | 1385篇 |
2015年 | 1839篇 |
2014年 | 2302篇 |
2013年 | 2792篇 |
2012年 | 2812篇 |
2011年 | 3000篇 |
2010年 | 2606篇 |
2009年 | 2249篇 |
2008年 | 2264篇 |
2007年 | 2060篇 |
2006年 | 1931篇 |
2005年 | 1590篇 |
2004年 | 1066篇 |
2003年 | 958篇 |
2002年 | 975篇 |
2001年 | 838篇 |
2000年 | 695篇 |
1999年 | 711篇 |
1998年 | 544篇 |
1997年 | 476篇 |
1996年 | 425篇 |
1995年 | 364篇 |
1994年 | 340篇 |
1993年 | 263篇 |
1992年 | 190篇 |
1991年 | 142篇 |
1990年 | 90篇 |
1989年 | 98篇 |
1988年 | 91篇 |
1987年 | 66篇 |
1986年 | 45篇 |
1985年 | 30篇 |
1984年 | 17篇 |
1983年 | 14篇 |
1982年 | 18篇 |
1981年 | 18篇 |
1980年 | 18篇 |
1979年 | 21篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1976年 | 9篇 |
1975年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 234 毫秒
31.
对赤红球菌的组氨酸激酶基因进行密码子优化,将优化后的组氨酸激酶基因(rhks)构建重组表达质粒pGEX-4T-2-rhks。将此质粒导入到大肠杆菌BL21(DE3)中进行异源表达。在25 ℃和1 mmol/L异丙基-β-D-硫代吡喃半乳糖苷诱导条件下,组氨酸激酶融合蛋白(GST-RHK)获得成功表达,并具有催化活性。经谷胱甘肽琼脂糖亲和层析纯化,获得电泳纯的GST-RHK,其中纯化倍数为3.1,得率为19.5%。该蛋白大小约为72.75 kDa,Km、Vmax和Kcat值分别为20.92 μmol/L、0.17 μmol/(L·min)和1.4 min-1。野生型赤红球菌、组氨酸激酶基因增强株sdrhkE和组氨酸激酶基因敲减株sdrhkD在分别含有苯酚、甲苯、氯苯、异辛烷4 种有机溶剂的培养基中培养,菌株sdrhkD的生长情况都优于野生型赤红球菌,菌株sdrhkE的生长情况都低于野生型赤红球菌。本研究为进一步揭示赤红球菌SD3中组氨酸激酶涉及的信号转导途径与赤红球菌有机溶剂耐受性的关联机制提供一定参考依据。 相似文献
32.
针对基于容积脉搏波(PPG)提取运动心率时,传统心率提取算法由于运动噪声干扰使测量结果误差大、实时性不好的问题,提出一种抗运动干扰的实时心率提取方法。该方法通过实时小波去噪,同时结合三轴加速度信号(ACC)对运动进行分类训练,计算各运动状态心率增益,对实时心率值进行补偿。实验结果表明,通过与同时采集的ECG信号计算出的实时心率进行对比,绝对误差率仅为1.2%左右。相比传统心率提取算法,该算法具有抗干扰性强,实时准确的特点。 相似文献
33.
Xiao-Guang Yue Guang Zhang Qu Wu Fei Li Xian-Feng Chen Gao-Feng Ren Mei Li 《稀有金属(英文版)》2015,(2):125-132
In order to predict the wearing of stellite alloys,the related methods of rare metals data processing were discussed. The method of opposite degree(OD) algorithm was put forward to predict the wearing of stellite alloys.OD algorithm is based on prior numerical data, posterior numerical data and the opposite degree between numerical forecast data. To compare the performance of predicted results based on different algorithms, the back propagation(BP) and radial basis function(RBF) neural network methods were introduced. Predicted results show that the relative error of OD algorithm is smaller than those of BP and RBF neural network methods. OD algorithm is an effective method to predict the wearing of stellite alloys and it can be applied in practice. 相似文献
34.
综采工作面在推进过程中经常会受到断层的影响,严重制约了矿井的安全生产及经济效益。以寺河煤矿西井区W2303工作面采用留顶煤、割底矸等手段过逆断层群为工程实例,分析了综采工作面安全、快速过逆断层群技术的应用效果,对于类似矿山也有一定的借鉴价值。 相似文献
35.
Qun Ren Haijun Su Jun Zhang Haifang Liu Weidan Ma Guangrao Fan Di Zhao Min Guo Lin Liu Hengzhi Fu 《Ceramics International》2019,45(6):6632-6638
Directionally solidified microstructures of Al2O3-Er3Al5O12 eutectic and off-eutectic in situ composite ceramics were explored under abrupt-change pulling rate conditions. Corresponding temperature distributions and interface locations were studied. In eutectic composition, fluctuation of eutectic spacing occurred when the pulling rate increased abruptly. A gradually increase or abrupt increase in eutectic spacing was observed when the pulling rate decreased abruptly. In hypoeutectic and hypereutectic compositions, formation of the primary phases were suppressed when the pulling rate increased abruptly from 10?µm/s to 100?µm/s, while primary phases precipitated when the pulling rate decreased abruptly from 100?µm/s to 10?µm/s. The interface altitude decreased after the pulling rate increased abruptly, but increased after the pulling rate decreased abruptly. The liquid composition restriction (around the eutectic composition) at the eutectic interface plays an important role in the suppression of the primary dendrite and coupled eutectic oxides can be obtained in off-eutectic compositions even under higher solidification rate conditions. 相似文献
36.
Li Lei Limin Li Shasha Wang Gaoyang Zhao Yoshifumi Oshima Yang Ren Lei Zhao Lihua Jin Yao Wang Chengshan Li Pingxiang Zhang 《Journal of the European Ceramic Society》2018,38(14):4694-4700
Highly (100)-oriented Ce1-x(Y0.2Zr0.8)xOδ (CYZO) films were prepared on biaxially textured NiW substrates by a chemical solution deposition approach using metal inorganic salts as starting materials. It has been found that both the preferential orientation and surface roughness of CYZO films decrease gradually with increasing of the doping percentage of Y3+ and Zr4+ ions. The epitaxial growth relationship of (220)CYZO//(200)NiW and [00?l]CYZO//[001]NiW was demonstrated by XRD texture measurement as well as atomic resolution STEM observation. XRD, Raman and XPS spectra results indicate that Y3+ and Zr4+ ions were indeed introduced into CeO2 lattice to substitute Ce4+ ions and form cubic fluorite CYZO solid solution. Moreover, CeO2 buffer layer can be endowed a strong enough capability to prevent element diffusion through co-doping of yttrium and zirconium, provided that an optimal doping ratio of them is adopted. This will provide a new approach to fabricating strong-barrier single buffer layer for coated conductor. 相似文献
37.
Wax deposit properties are a significant concern in pipeline pigging during waxy crude oil transportation. In the present work, the impacts of flow conditions and oil properties on the wax precipitation characteristics of wax deposits are investigated. A flow loop apparatus was developed to conduct wax deposition experiments using four crude oils collected from different field pipes. The differential scanning calorimetry (DSC) technique was employed to observe the wax precipitation characteristics of crude oil and wax deposit. The results show that the wax content and the wax appearance temperature (WAT) of the deposits increase with shear stress and radial temperature gradient, and decrease with radial wax molecule concentration gradient near the pipe wall. The DSC tests on the wax deposits revealed that the deposit wax content is strongly correlated to the oil wax content. Furthermore, an empirical correlation was developed to predict the wax content and the WAT of the wax deposit. Verification of the empirical correlation using the different oils indicated that the average relative error of the wax content prediction and average absolute error of WAT prediction were 13.2% and 3.6°C, respectively. 相似文献
38.
39.
Jianmou Lu Yanli Huang Shunyan Ren 《International Journal of Control, Automation and Systems》2020,18(5):1250-1263
This paper investigates the general decay synchronization and general decay H∞ synchronization problems of multi-weighted coupled reaction-diffusion 相似文献
40.
Ningning Fu Jiaxing Li Ming Wang Lili Ren Youqing Luo 《International journal of molecular sciences》2020,21(22)
An obligate mutualistic relationship exists between the fungus Amylostereum areolatum and woodwasp Sirex noctilio. The fungus digests lignin in the host pine, providing essential nutrients for the growing woodwasp larvae. However, the functional properties of this symbiosis are poorly described. In this study, we identified, cloned, and characterized 14 laccase genes from A. areolatum. These genes encoded proteins of 508 to 529 amino acids and contained three typical copper-oxidase domains, necessary to confer laccase activity. Besides, we performed molecular docking and dynamics simulation of the laccase proteins in complex with lignin compounds (monomers, dimers, trimers, and tetramers). AaLac2, AaLac3, AaLac6, AaLac8, and AaLac10 were found that had low binding energies with all lignin model compounds tested and three of them could maintain stability when binding to these compounds. Among these complexes, amino acid residues ALA, GLN, LEU, PHE, PRO, and SER were commonly present. Our study reveals the molecular basis of A. areolatum laccases interacting with lignin, which is essential for understanding how the fungus provides nutrients to S. noctilio. These findings might also provide guidance for the control of S. noctilio by informing the design of enzyme mutants that could reduce the efficiency of lignin degradation. 相似文献