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排序方式: 共有313条查询结果,搜索用时 17 毫秒
301.
Extraction of functional ingredients from spinach (Spinacia oleracea L.) using liquid solvent and supercritical CO2 extraction 下载免费PDF全文
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Rosalba Siracusa Francesco Monaco Ramona DAmico Tiziana Genovese Marika Cordaro Livia Interdonato Enrico Gugliandolo Alessio Filippo Peritore Rosalia Crupi Salvatore Cuzzocrea Daniela Impellizzeri Roberta Fusco Rosanna Di Paola 《International journal of molecular sciences》2021,22(13)
Treating postoperative (PO) pain is a clinical challenge. Inadequate PO pain management can lead to worse outcomes, for example chronic post-surgical pain. Therefore, acquiring new information on the PO pain mechanism would increase the therapeutic options available. In this paper, we evaluated the role of a natural substance, epigallocatechin-3-gallate (EGCG), on pain and neuroinflammation induced by a surgical procedure in an animal model of PO pain. We performed an incision of the hind paw and EGCG was administered for five days. Mechanical allodynia, thermal hyperalgesia, and motor dysfunction were assessed 24 h, and three and five days after surgery. At the same time points, animals were sacrificed, and sera and lumbar spinal cord tissues were harvested for molecular analysis. EGCG administration significantly alleviated hyperalgesia and allodynia, and reduced motor disfunction. From the molecular point of view, EGCG reduced the activation of the WNT pathway, reducing WNT3a, cysteine-rich domain frizzled (FZ)1 and FZ8 expressions, and both cytosolic and nuclear β-catenin expression, and the noncanonical β-catenin–independent signaling pathways, reducing the activation of the NMDA receptor subtype NR2B (pNR2B), pPKC and cAMP response element-binding protein (pCREB) expressions at all time points. Additionally, EGCG reduced spinal astrocytes and microglia activation, cytokines overexpression and nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) pathway, downregulating inducible nitric oxide synthase (iNOS) activation, cyclooxygenase 2 (COX-2) expression, and prostaglandin E2 (PGE2) levels. Thus, EGCG administration managing the WNT/β-catenin signaling pathways modulates PO pain related neurochemical and inflammatory alterations. 相似文献
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Tiziana Genovese Marika Cordaro Rosalba Siracusa Daniela Impellizzeri Sebastiano Caudullo Emanuela Raffone Francesco Macrí Livia Interdonato Enrico Gugliandolo Claudia Interlandi Rosalia Crupi Ramona DAmico Roberta Fusco Salvatore Cuzzocrea Rosanna Di Paola 《International journal of molecular sciences》2022,23(10)
Endometriosis is usually associated with inflammation and chronic pelvic pain. This paper focuses the attention on the anti-inflammatory, anti-oxidant and analgesic effects of cannabidiol (CBD) and on its potential role in endometriosis. We employed an in vivo model of endometriosis and administered CBD daily by gavage. CBD administration strongly reduced lesions diameter, volume and area. In particular, it was able to modify lesion morphology, reducing epithelial glands and stroma. CBD showed anti-oxidant effects reducing lipid peroxidation, the expression of Nox-1 and Nox-4 enzymes. CBD restored the oxidative equilibrium of the endogenous cellular defense as showed by the SOD activity and the GSH levels in the lesions. CBD also showed important antifibrotic effects as showed by the Masson trichrome staining and by downregulated expression of MMP-9, iNOS and TGF-β. CBD was able to reduce inflammation both in the harvested lesions, as showed by the increased Ikb-α and reduced COX2 cytosolic expressions and reduced NFkB nuclear localization, and in the peritoneal fluids as showed by the decreased TNF-α, PGE2 and IL-1α levels. CBD has important analgesic effects as showed by the reduced mast cells recruitment in the spinal cord and the reduced release of neuro-sensitizing and pro-inflammatory mediators. In conclusion, the collected data showed that CBD has an effective and coordinated effects in endometriosis suppression. 相似文献
305.
Anna Signorile Consiglia Pacelli Luigi Leonardo Palese Arcangela Santeramo Emilio Roca Tiziana Cocco Domenico De Rasmo 《International journal of molecular sciences》2022,23(17)
The oxidative phosphorylation (OXPHOS) system couples the transfer of electrons to oxygen with pumping of protons across the inner mitochondrial membrane, ensuring the ATP production. Evidence suggests that respiratory chain complexes may also assemble into supramolecular structures, called supercomplexes (SCs). The SCs appear to increase the efficiency/capacity of OXPHOS and reduce the reactive oxygen species (ROS) production, especially that which is produced by complex I. Studies suggest a mutual regulation between complex I and SCs, while SCs organization is important for complex I assembly/stability, complex I is involved in the supercomplex formation. Complex I is a pacemaker of the OXPHOS system, and it has been shown that the PKA-dependent phosphorylation of some of its subunits increases the activity of the complex, reducing the ROS production. In this work, using in ex vivo and in vitro models, we show that the activation of cAMP/PKA cascade resulted in an increase in SCs formation associated with an enhanced capacity of electron flux and ATP production rate. This is also associated with the phosphorylation of the NDUFS4 subunit of complex I. This aspect highlights the key role of complex I in cellular energy production. 相似文献
306.
Tiziana Schioppa Hoang Oanh Nguyen Laura Tiberio Francesca Sozio Carolina Gaudenzi Mauro Passari Annalisa Del Prete Daniela Bosisio Valentina Salvi 《International journal of molecular sciences》2022,23(17)
Histone deacetylase inhibitors (HDIs) are promising drugs for the treatment of inflammatory diseases. However, their therapeutical exploitation is slowed down by severe adverse manifestations that can hardly be foreseen, mainly due to incomplete knowledge of how HDIs impact the delicate balance of inflammatory mediators. In this work, we characterized the effects of the HDI trichostatin A (TSA) on the expression of TNFAIP3, which is a crucial inhibitor of the classical NF-kB pathway and an LPS-induced negative feedback regulator. The accumulation of TNFAIP3 mRNA after LPS stimulation showed biphasic behavior, with one wave within the first hour of stimulation and a second wave several hours later, which were both reduced by TSA. By using inhibition and knockdown approaches, we identified two temporally and mechanistically distinct modes of action. The first wave of TNAIP3 accumulation was directly blunted by the histone deacetylase (HDAC) blockade. By contrast, the second wave was decreased mainly because of the lack of endogenous TNF-α induction, which, in turn, depended on the intact HDAC activity. In both cases, class I HDACs appeared to play a nonredundant role, with HDAC3 required, but not sufficient, for TNF-α and TNFAIP3 induction. In addition to TNFAIP3, TNF-α is known to induce many response genes that orchestrate the inflammatory cascade. Thus, suppression of TNF-α may represent a general mechanism through which HDIs regulate a selected set of target genes. 相似文献
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Andrea Stefani Tommaso Salzillo Patrizia Romana Mussini Tiziana Benincori Massimo Innocenti Luca Pasquali Andrew C. Jones Suryakant Mishra Claudio Fontanesi 《Advanced functional materials》2024,34(2):2308948
In this paper it is experimentally demonstrated that the electron-spin/molecular-handedness interaction plays a fundamental role in the chiral recognition process. This conclusion is inferred comparing current versus potential (I–V) curves recorded using chiral electrode surfaces, which are obtained via chemisorption of an enantiopure thiophene derivative: 3,3′-bibenzothiophene core functionalized with 2,2′-bithiophene wings (BT2T4). The chiral recognition capability of these chiral-electrodes is probed via cyclic voltammetry measurements, where, Ag nanoparticles (AgNPs) capped with enantiopure BT2T4 (BT2T4@AgNP) are used as the chiral redox probe. Then, the interface handedness is explored by recording spin-polarized I–V curves in spin-dependent electrochemistry (SDE) and magnetic-conductive atomic force microscopy (mc-AFM) experiments. The quality of the interfaces is thoroughly cross-checked using X-ray photoemission spectroscopy, Raman, electrodesorption measurements, which further substantiate the metal(electrode)-sulfur(thiophene) central role in the chemisorption process. Spin-polarization values of about 15% and 30% are obtained in the case of SDE and mc-AFM experiments, respectively. 相似文献
309.
There are two common approaches for the detection of Genetically Modified Organisms (GMOs): DNA based methods, mainly founded on the Polymerase Chain Reaction (PCR), which detect genetically modified DNA sequences, and protein based methods, relying on immune assays (e. g. Enzyme‐Linked Immunosorbent Assay, ELISA). The latter detect and measure levels of proteins expressed by transgenic genes. Official standard tests, yet to come, will be based on these two methodologies. DNA based tests are now preferred for their sensitivity and their capability to detect a wider range of constructs. Protein‐based immune assay tests, although less sensitive, are quicker and require less lab skills. Collaborative study results put in evidence that PCR tests are generally well suited for detecting the presence of GMOs on a qualitative basis (yes/no). Difficulties arise when GMOs have to be quantitatively identified in food ingredients. Real‐Time, or kinetic PCR, points out quantification and interpretation limits when quantification has to be done on a very small total DNA amount. An essential requirement for PCR‐based techniques is the knowledge of the GMO‐specific DNA sequence target. Many labs find it difficult to keep up with the rate at which life science companies are creating new GMOs and the finding of adequate reference standards to be used as positive analytical controls. 相似文献
310.