The method of intraoperative intestinal dialysis in peritonitis

Psychological testing of 63 normal subjects and 84 patients with keratocone was carried out separately for men and women aged 18-64 years using 11 scores of a brief multifactorial questionnaire for personality examination (BMQP). Comparison of averaged profiles of examined men and women with keratocone showed features of sensitive and psychasthenic personalities. According to BMQP scores, the population of men with keratocone is characterized by increased levels of psychasthenia and schizophrenia, whereas women develop depression, psychasthenia, and schizophrenia.     

The treatment of intra-abdominal abscess

The experience of treatment of 18 patients with intraabdominal abscess using the regional laparostomy conduction and introducing nitazol, immobilized on polymethylsiloxane, in the abscess cavity, was summarized.     

A new PCR-primer for specific amplification of human DNA fragments selected on the basis of computer analysis of the nucleotide sequences of MER1 dispersed repeats in man

A HPLC method with coulometric detection has been established to carry out the separation of the three nitrofuran derivatives, nitrofurantoin, furazolidone and furaltadone. A Nova-Pak C18 column (150 x 3.9 mm) and a Coulochem II detector from ESA have been used. After obtaining the hydrodynamic curves of the three compounds in the porous graphite electrode a potential of -600 mV was selected as the working potential. The influence of other variables such as mobile phase composition and flow-rate were studied. The mobile phase considered as an optimum was acetonitrile-0.1 M aqueous solution of sodium perchlorate (28:72), with 0.5% glacial acetic acid. The oxygen of the mobile phase was removed with a vacuum system on-line and a nitrogen stream was used to remove the oxygen of the samples. The calibration graphs and the detection limits were established. The method proposed was used, with good results, for the determination of the three compounds in milk.     

Changes in the reactivity of the sensorimotor cortex resulting from the treatment of patients with depressive disorders

Changes in the mu-rhythm power in response to sound (non-specific reactivity, NR) and related with the actual and imaginary movement with the right and left hand in response to sound (specific reactivity, SR) were studied in 38 right-handed patients with subdepressive psychogenic (PD) and endogenous (ED) disorders before and after successful complex treatment (pharmacotherapy and acupuncture) in comparison with 23 healthy subjects. The successful treatment was found to be accompanied by a certain extent of normalization of the disturbed SR which was different in PD and ED. The more complete recovery of the normal reactions in PD was mainly accounted for by the relative decrease in the NR and increase in the SR. The ED treatment predominantly modified the SR. Substantial reorganization of interhemispheric relations during movements in the PD patients resulted in disappearance of the abnormal ipsilateral reactions and transition to the contralateral reactions of the sensorimotor cortex.     

Thyrotropin-releasing activity of histone H2A, H2B and peptide MB35

Histones possess multiple hormone-like activities. We studied the specificity and signal transduction pathways involved in the thyrotrophin (TSH)-releasing activity of histones H2A, H2B and peptide MB35, a H2A fragment, using perifused and incubated dispersed rat pituitary cells and measuring TSH release by a specific R1A. Histones released TSH in a dose- and time-dependent fashion while peptide MB35 behaved as a weaker secretagogue. Trifluoperazine and EGTA blocked histone-stimulated TSH release while forskolin and other cAMP enhancers did not. We conclude that the TSH-releasing activity of histones H2A and H2B is mediated by calcium- and diacylglycerol-associated pathways.     

Effect of metal cations on the copper induced peroxidation of the low density lipoproteins

The effect of metal cations on copper-catalyzed lipid peroxidation (LPO) of low density lipoproteins (LDL) was examined. The presence of metal cations in the incubation media containing LDL (0.8 mg protein/ml) and CuSO4 (0-80 microM) influenced on LPO of LDL as evident by the measurement of TBARS. With the concentrations of CuSO4 less than 10 microM, the metal cations caused an increase in LDL peroxidation. Zn2+ appeared to be the most effective inductor, Mn2+ was less effective, and the influence of Ca2+ and Mg2+ was insignificant. With greater CuSO4 concentrations Mg2+ showed no effect on TBARS formation in LDL while the addition of other nontransition metal cations to the incubation mixture led to the inhibition of LDL peroxidation. The capacity for inhibition decreased in the row Mn2+ > Zn2+ > Ca2+ > Mg2+. The possible mechanism explaining these results may be in the competition of metal ions for copper binding sites on LDL. Our results allow to suggest the existence of two types of copper binding sites on LDL, tight-binding sites which are non-effective in LPO and effective weak-binding sites.     

Effect of splenin and its fractions on the functional state of the adrenal cortex

Effect of splenin (1 ml) and its fractions (0.5 ml) on adrenocortical function was studied after a single intramuscular injection of the preparations into rats. Splenin and its fractions containing water-soluble substances reduced the content of ascorbic acid in the rat adrenals thereby indicating the increased synthesis of adrenocortical hormones. Adenosine-5-monophosphate is one of the basic biological substances contained by splenin and its water-soluble fractions. It produces a stimulant effect on the synthesis of adrenocortical hormones.