Phylogenetic analysis of Rhodococcus erythropolis based on the variation of ribosomal proteins as observed by matrix-assisted laser desorption ionization-mass spectrometry without using genome information

Rhodococcus erythropolis strains characterized as antibiotic producers can be classified into three groups according to their antibiotic spectrum and growth compatibility. Due to their high genotypic similarity, the taxonomic relationship of these strains has not been elucidated. In this study, ribosomal protein profiling using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) was employed to classify twenty-one strains of R. erythropolis (15 antibiotic producers and 6 non-antibiotic producers). In the first step in this method, a total of 30 intense peaks observed for purified ribosomal subunit proteins of the type strain (R. erythropolis JCM 3201^T) were selected as the reference peaks. The mass spectra observed for the cell lysates of each sample strain were then checked as to whether peaks were observed at the same masses of the reference peaks. The results of peak matching were processed by cluster analysis, generating a dendrogram. Four major clusters of the R. erythropolis strains corresponded to three antibiotic groups and the non-antibiotic group. Furthermore, the topology of the dendrogram was highly comparable with the phylogenetic tree based on DNA gyrase subunit B gene (gyrB) sequencing. These results indicate that our proposed ribosomal protein profiling method using MALDI-MS is a potentially reliable and sufficiently high-throughput technique for the taxonomic analysis of closely related bacterial strains without using DNA sequence information.     

Numerical analysis of characteristics of microactuators driven by liquid crystals

For the purpose of developing liquid crystalline microactuators, the transient behaviors of a nematic liquid crystal between two parallel plates have been computed for various parameters such as applied voltage, the gap between the plates, and the twist and tilt angles at the plates. The Leslie-Ericksen theory has been selected as a constitutive equation. The twist angle has an effect on the induced velocity profiles; for example, the induced flow is planar at the twist angle of 0°, while the flow has an out-of-plane component when the twist angle is not 0°. Transient behaviors of shear stress acting on the plates, the flowrate, and the maximum values of the velocity, and the tilt angle between the plates have been reported. In addition, we have investigated the effects of the applied voltage, the gap between the plates, and the tilt angle at the plates on the above-mentioned values. We can develop microactuators with arbitrary characteristics by suitably controlling the applied voltage, the size of the actuators, and the director anchoring conditions.     

Fabrication of submicron alumina ceramics by pulse electric current sintering using M2+ (M = Mg,Ca, Ni)-doped alumina nanopowders

Dense submicron-grained alumina ceramics were fabricated by pulse electric current sintering (PECS) using M²⁺(M: Mg, Ca, Ni)-doped alumina nanopowders at 1250 °C under a uniaxial pressure of 80 MPa. The M²⁺-doped alumina nanopowders (0–0.10 mass%) were prepared through a new sol–gel route using high-purity polyhydroxoaluminum (PHA) and MCl₂ solutions as starting materials. The composite gels obtained were calcined at 900 °C and ground by planetary ball milling. The powders were re-calcined at 900 °C to increase the content of α-alumina particles, which act as seeding for low-temperature densification. Densification and microstructural development depend on the M²⁺ dopant species. Dense alumina ceramics (relative density ≥99.0%) thus obtained had a uniform microstructure composed of fine grains, where the average grain size developed for non-doped, Ni-doped, Mg-doped and Ca-doped samples was 0.67, 0.67, 0.47 and 0.30 μm, respectively, showing that Ca-doping is the most promising method for tailoring of nanocrystalline alumina ceramics.     

Functional metal-porphyrazine derivatives and their polymers, 10. Secondary fuel cells based on oxygen reduction on platinum electrode modified by polymers and metal-phthalocyanines

Secondary fuel cells based on oxygen reduction of platinum electrode modified by polymers and metal-phthalocyanine (Mt = Fe(III), Co(II), Ni(II), and Cu(II)) were studied. The discharge curves for the platinum electrode modified by poly(2-vinylpyridine) (or polystyrene) and Co-phthalocyanine in 30% KOH aqueous solution, for a 30 min charge at 500 μA, followed by a 100 μA discharge showed a stable plateau at about ?0.24 V SCE (Saturated Calomel Electrode). The open circuit voltage (vs. Zn) of the cell was 1.2 V, and the discharge capacity was of 46 A · h/kg. For this battery there was no significant decay in its characteristics after more than 30 charge-discharge cycles. In Mt-phthalocyanines, the values decreased in the order of Co(II) > Fe(III) > > Cu(II) > Ni(II). From a cyclic voltammogram for the electrode modified by the polymer and Co-Pc, the cathodic reactions were discussed.     

Characteristic Property of Low Bitterness in Protein Hydrolysates by a Novel Soybean Protease D3

ABSTRACT:  Enzymatic hydrolysis is 1 means of improving the functional properties of food protein; however, in most cases, bitter peptides are generated by such treatment, and the resulting product is therefore not acceptable as a food ingredient. We have already reported a novel cysteine protease, D3, purified from germinating soybean cotyledons. Because of its substrate specificities, most hydrophobic amino acid residues in the hydrolysate are presumed not to be located at the peptide termini. It was therefore expected that protein hydrolysate by protease D3 would taste less bitter than other enzymatic hydrolysates. The objective of this study was to demonstrate the low bitterness of protein hydrolysates by protease D3. For that purpose, soy protein and casein hydrolysates were prepared with treatment of protease D3, subtilisin, pepsin, trypsin, and thermolysin, respectively. The bitterness of these hydrolysates was evaluated by measuring points of subjective equality (PSE). The PSE value demonstrated that the protein hydrolysates by protease D3 were significantly less bitter than the other enzymatic hydrolysates, indicating that the products had a taste mild enough to be acceptable as a less-bitter peptide food ingredient. These results suggested that a prominent feature of protease D3 was its capacity to produce less-bitter peptides. Therefore, it is thought that protease D3 could be applied to produce protein hydrolysates for use as ingredients in a variety of food products.     

Immunohistochemical study of calpain-mediated alpha-crystallin proteolysis in the UPL rat hereditary cataract

The purpose of this study is to examine if microencapsulated PC12 cells may provide long term effects to the hemiparkinsonian rats. A modified technique was used to encapsulate PC12 cells into gelled microspheres. We found that the PC12 cells can survive in the modified microcapsules in vitro. Most of the PC12 cells formed cluster 3 weeks after incubation. The PC12 cell-loaded microcapsules were also examined in vitro. Adult Sprague-Dawley rats, anesthetized with chloral hydrate, were injected unilaterally with 6-hydroxydopamine into the medial forebrain bundle. The effectiveness of this lesion was tested by measuring apomorphine or methamphetamine-induced rotation one month after lesioning. The unilaterally lesioned rats were transplanted with microencapsulated PC12 cells. Results showed that apomorphine and methamphetamine-induced rotations were greatly suppressed after transplantation. One year after the grafting, the animals were anesthetized with urethane for the voltammetric study. Low dose of KCl was directly injected into the grafted striatum through pressure microejection. We found that KCl-induced DA release, as measured by voltammetric techniques, was regenerated in the striatum. The animals were later sacrificed for histological examination. We found that capsules were present in the lesioned striatum one year after grafting. Most of the capsules contained no PC12 cell. However, some capsules were filled entirely with PC12 cells. Taken together, our data suggested that PC12 cells can survive in the capsule in vitro and may provide long-term dopaminergic effects to the hemiparkinsonian rats.     

Mode analysis of spreading of partially coherent beams propagating through atmospheric turbulence

The spreading of partially coherent beams propagating through atmospheric turbulence is studied by use of the coherent-mode representation of the beams. Specifically, we consider partially coherent Gaussian Schell-model beams entering the atmosphere, and we examine the spreading of each coherent mode, represented by a Hermite-Gaussian function, on propagation. We find that in atmospheric turbulence the relative spreading of higher-order modes is smaller than that of lower-order modes, whereas the relative spreading of all order modes is the same as in free space. This modal behavior successfully explains why under certain circumstances partially coherent beams are less affected by atmospheric turbulence than are fully spatially coherent laser beams.     

Mechanical behavior of a single polymer chain in a non-solvent

Mechanical properties of a single polystyrene chain in mixtures of dioxane and methanol were measured with AFM. The effect of the solubility of the surrounding liquid on mechanical behavior of a polymer chain was examined. In good and Θ solvents, the force-extension curves exhibit a freely jointed chain (FJC)-like trend with good reproducibility. In a non-solvent, the profile of force-extension curve was dependent on the extension speed: an FJC-like nature emerged at the lower speed of 200 nm/s while saw-toothed curves were obtained at the higher speed of 2000 nm/s. The shape of saw-toothed curves varied from measurement to measurement. A force relaxation was also observed in the non-solvent under a fixed extension distance after 2000 nm/s extension. The mechanical behavior in non-solvents suggests that inhomogeneous deformation of a PS chain occurs due to reduction of the chain mobility.     

Improvement of anode oxidation reaction of a fuel cell using ammonium formate with Pt‐Ir catalysis

We have attempted to develop high‐performance and safe fuel cells by using ammonium formate as a solid (powder) fuel. This solid fuel has the potential of safer transportability than liquid fuels such as methanol from the viewpoint of toxicity and flammability. In order to make use of some of the advantages of ammonium formate, we investigated the oxidation characteristics of ammonium formate with respect to a Pt electrode. Cyclic voltammograms indicate that ammonium formate has high oxidation activity with respect to a Pt electrode. We have also found that the oxidation of ammonium formate can be improved by the addition of Ir to Pt catalysts. It is highly likely that ammonium formate will be useful as a solid (powder) fuel for polymer electrolyte membrane (PEM) fuel cells. This new fuel will promote the development of safe fuel cells for PEM. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 174(4): 45–50, 2011; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.21043