Development and Application of Multiple‐Probe Scanning Probe Microscopes

In the research of advanced materials based on nanoscience and nanotechnology, it is often desirable to measure nanoscale local electrical conductivity at a designated position of a given sample. For this purpose, multiple‐probe scanning probe microscopes (MP‐SPMs), in which two, three or four scanning tunneling microscope (STM) or atomic force microscope (AFM) probes are operated independently, have been developed. Each probe in an MP‐SPM is used not only for observing high‐resolution STM or AFM images but also for forming an electrical contact enabling nanoscale local electrical conductivity measurement. The world's first double‐probe STM (DP‐STM) developed by the authors, which was subsequently modified to a triple‐probe STM (TP‐STM), has been used to measure the conductivities of one‐dimensional metal nanowires and carbon nanotubes and also two‐dimensional molecular films. A quadruple‐probe STM (QP‐STM) has also been developed and used to measure the conductivity of two‐dimensional molecular films without the ambiguity of contact resistance between the probe and sample. Moreover, a quadruple‐probe AFM (QP‐AFM) with four conductive tuning‐fork‐type self‐detection force sensing probes has been developed to measure the conductivity of a nanostructure on an insulating substrate. A general‐purpose computer software to control four probes at the same time has also been developed and used in the operation of the QP‐AFM. These developments and applications of MP‐SPMs are reviewed in this paper.     

Identification of a Molecular Target of a Novel Fungal Metabolite,Pyrrolizilactone, by Phenotypic Profiling Systems

In the course of screening our microbial metabolite fraction library, we identified a novel pyrrolizidinone compound, pyrrolizilactone. In this study, we report the identification and characterization of a molecular target for pyrrolizilactone by using two phenotypic profiling systems. Cell morphology‐based profiling analysis using an imaging cytometer (MorphoBase) classified pyrrolizilactone as a proteasome inhibitor. Consistently, proteome‐based profiling analysis using 2D difference gel electrophoresis (DIGE; ChemProteoBase) also demonstrated that pyrrolizilactone is associated with proteasome inhibition. On the basis of these predictions, we determined that pyrrolizilactone is a novel type of proteasome inhibitor inhibiting the trypsin‐like activity of the proteasome.     

A study on modeling methodology of oligopolistic virtual market and its application into resource allocation problem

Recently, manufacturing systems have become large scale and complex. The social scientific approach is one of the promising methods to facilitate effective operation for the resource allocation problems in manufacturing systems, since the approach takes an analogy of social systems which are optimal and robust in nature. In this paper, the authors first propose a modeling methodology for an oligopolistic virtual market, and develop an oligopolistic virtual market as an experimental model. Then they clarify several important characteristics of the oligopolistic virtual market by computer simulation experiments, and try to apply the proposed method into a utility computing operational problem to validate its effectiveness into resource allocation problems. © 2008 Wiley Periodicals, Inc. Electr Eng Jpn, 164(1): 77–85, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20628     

A solid electrolyte nanometer switch

We have investigated solid electrolyte switches that utilize electrochemical reactions (deposition and dissolution) of metallic ions. The switch turns off or on when a metallic bridge electrochemically forms or dissolves in the solid electrolyte. Each state is nonvolatile and the switching is repeatable up to 10⁵ cycles. The promising application is a programmable switch in a field programmable logic because of its small size (<30 nm) and low ON resistance (<100Ω). This paper discusses the electrical characteristics, operation principle, and applications of the solid electrolyte switch. © 2008 Wiley Periodicals, Inc. Electr Eng Jpn, 165(1): 68–73, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20542     

Quantification of the number of EP3 receptors on a living CHO cell surface by the AFM

The distribution of EP3 receptors on a living cell surface was quantitatively studied by atomic force microscopy (AFM). Green fluorescent protein (GFP) was introduced to the extracellular region of the EP3 receptor on a CHO cell. A microbead was used as a probe to ensure certain contact area, whose surface was coated with anti-GFP antibody. The interactions between the antibodies and GFP molecules on the cell surface were recorded to observe the distribution of the receptors. The result indicated that EP3 receptors were distributed on the CHO cell surface not uniformly but in small patches coincident with immunohistochemical observation. Repeated measurements on the same area of cell surface gave confirmation that it was unlikely that the receptors were extracted from the cell membrane during the experiments. The measurement of single molecular interaction between GFP and the anti-GFP antibody was succeeded on the cell surface using compression-free force spectroscopy. The value of separation work required to break a single molecular pair was estimated to be about 1.5 x 10(-18)J. The number of EP3 receptor on the CHO cell surface was estimated using this value to be about 1 x 10(4) under the assumption that the area of the cell surface was about 5,000 microm(2). These results indicated that the number of receptors on a living cell surface could be quantified through the force measurement by the AFM.     

Analysis of power flow in a power converter

Power converters have been widely used in many industrial applications. On the ac side of such converters, the current is distorted because of nonlinearity of the power devices even when the source voltage is sinusoidal. This implies that the power converter is regarded as a harmonic-current source. The harmonic current is one of the most important factors in the analysis of converters. This paper analyzes power flow of a power converter that behaves synchronously with the line frequency. Power-flow analysis in a three-phase diode converter with a resistance on the ac side is presented. The analysis is compared with conventional analysis with the assumption that the ratio of the resistance on the ac side to that on the dc side is very small. The proposed power flow concept enables electrical engineers and students to discuss rationally the power flow of a converter. © 1997 Scripta Technica, Inc. Electr Eng Jpn, 120(1): 70–76, 1997     

Design of Ceramic Materials for Chemical Sensors: SmFeO3 Thick Films Sensitive to NO2

Ultrafine SmFeO₃ powders were prepared by the thermal decomposition at 700°C of the corresponding hexacyanocomplex, Sm[Fe(CN)₆]·4H₂O. These powders were used for the preparation of pastes which were deposited as thick films on alumina substrates with comb-type Au electrodes. The films were fired at different temperatures in the 800-1000°C range. The content of α-terpineol, a component of the organic vehicle, was varied in the range 0.0046-4 wt%. The microstructure, the chemical composition at the surface, the electrical conductivity, and the NO₂ sensing properties of the films were investigated. The content of α-terpineol strongly influenced the electrical conductivity and its activation energy. A significant reduction in the NO₂ response was observed for the films containing smaller amounts of α-terpineol, together with an increase in conductivity. On the other hand, the largest NO₂ response was observed for the films fired at 1000°C when 4 wt% of α-terpineol was used. Such increase in conductivity is attributed to a different oxygen surface layer on the SmFeO₃ surface, which is induced by the decomposition reaction of α-terpineol during sintering. The materials processing parameters are thus of primary concern for the NO₂ sensing properties of the SmFeO₃ thick films. The correlations found between activation energy, NO₂ sensitivity, and materials characteristics (influenced by the preparation parameters) are reported. These correlations can be used to design the gas-sensing properties of SmFeO₃ thick films for the optimization of their sensing characteristics.     

Influence of diet on fatty acids of three subtropical fish, subfamily caesioninae (Caesio diagramma and C. tile) and family siganidae (Siganus canaliculatus)

The total lipid and fatty acid compositions of tissues and the stomach contents of three subtropical marine fish species, subfamily Caesioninae, Caesio diagramma and C. tile, and family Siganidae Siganus canaliculatus, were investigated to clarify the differences between these species. Triacylglycerols (TAG) were the dominant depot lipids of the three species, whereas wax esters were found as a minor component. In particular, muscle lipids were found to contain mainly glycerol derivatives such as TAG and phospholipids. The major fatty acids identified in the three species were 16∶0, 18∶0, 18∶1n−9, and 22∶6n−3 (docosahexaenoic acid, DHA). In addition, noticeable levels of 16∶1n−7, 18∶1n−7, 20∶4n−6 (arachidonic acid, AA), and 20∶5n−3 (eicosapentaenoic acid) were found. DHA was the most abundant polyunsaturated fatty acid (PUFA) in the muscle and viscera lipids of the three species. The high DHA levels in the lipids of all the organs were found to be higher than those of the lipid extracted from the stomach contents of the three fishes. In addition, the specimens of S. canaliculatus contained significantly higher levels of AA in its tissues than did the other two species. A high AA content is unusual since such high levels of n−6 PUFA are rarely found in higher marine organisms. These levels may be due to its characteristic feeding pattern, because S. canaliculatus prefer and mainly feed on seaweed, which often contains high amounts of n−6 PUFA, such as linoleic acid (18∶2n−6) and AA.