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61.
Jason K. Iles Raminta Zmuidinaite Christoph Sadee Anna Gardiner Jonathan Lacey Stephen Harding Gregg Wallis Roshani Patel Debra Roblett Jonathan Heeney Helen Baxendale Ray Kruse Iles 《International journal of molecular sciences》2022,23(11)
The involvement of immunoglobulin (Ig) G3 in the humoral immune response to SARS-CoV-2 infection has been implicated in the pathogenesis of acute respiratory distress syndrome (ARDS) in COVID-19. The exact molecular mechanism is unknown, but it is thought to involve this IgG subtype’s differential ability to fix, complement and stimulate cytokine release. We examined the binding of convalescent patient antibodies to immobilized nucleocapsids and spike proteins by matrix-assisted laser desorption/ionization–time of flight (MALDI-ToF) mass spectrometry. IgG3 was a major immunoglobulin found in all samples. Differential analysis of the spectral signatures found for the nucleocapsid versus the spike protein demonstrated that the predominant humoral immune response to the nucleocapsid was IgG3, whilst for the spike protein it was IgG1. However, the spike protein displayed a strong affinity for IgG3 itself, as it would bind from control plasma samples, as well as from those previously infected with SARS-CoV-2, similar to the way protein G binds IgG1. Furthermore, detailed spectral analysis indicated that a mass shift consistent with hyper-glycosylation or glycation was a characteristic of the IgG3 captured by the spike protein. 相似文献
62.
Jorge Lopez-Tello Maria Angeles Jimenez-Martinez Esteban Salazar-Petres Ritik Patel Amy L. George Richard G. Kay Amanda N. Sferruzzi-Perri 《International journal of molecular sciences》2022,23(11)
Pregnancy is characterized by adaptations in the function of several maternal body systems that ensure the development of the fetus whilst maintaining health of the mother. The renal system is responsible for water and electrolyte balance, as well as waste removal. Thus, it is imperative that structural and functional changes occur in the kidney during pregnancy. However, our knowledge of the precise morphological and molecular mechanisms occurring in the kidney during pregnancy is still very limited. Here, we investigated the changes occurring in the mouse kidney during pregnancy by performing an integrated analysis involving histology, gene and protein expression assays, mass spectrometry profiling and bioinformatics. Data from non-pregnant and pregnant mice were used to identify critical signalling pathways mediating changes in the maternal kidneys. We observed an expansion of renal medulla due to proliferation and infiltration of interstitial cellular constituents, as well as alterations in the activity of key cellular signalling pathways (e.g., AKT, AMPK and MAPKs) and genes involved in cell growth/metabolism (e.g., Cdc6, Foxm1 and Rb1) in the kidneys during pregnancy. We also generated plasma and urine proteomic profiles, identifying unique proteins in pregnancy. These proteins could be used to monitor and study potential mechanisms of renal adaptations during pregnancy and disease. 相似文献
63.
A study on the thermal analysis of amylose and some of its derivatives viz., amylose-palmitate, -succinate and sodium carboxymethyl amylose (NaCMA) has been carried out. The TG and DTA data have been analyzed using the methods of Chatterjee and Reich. The Chatterjee method adequately explained the initial part of the decomposition, whereas overall activation energy has been obtained by Reich's method. The results indicated that except with amylose succinate the esterification increased the thermal stability of all the amylosic derivatives compared to amylose. NaCMA showed relatively low thermal stability. The following order of stability was noted: amylose palmitate > amylose acetate > amylose propionate > amylose > NaCMA > amylose-succinate. 相似文献
64.
The performance of active queue management (AQM) is measured in terms of throughput, delay, queue size, and loss rate. We have carried out the optimized performance measure of throughput for AQM scheme random early detection (RED) using full factorial design (FDD) technique that is a new approach of performance analysis particularly for congestion control algorithms. We have considered the input factors, viz, buffer size, maximum threshold, and the number of file transfer protocol (FTP) sources for the evaluation of RED that can be used for other AQM schemes, viz, adaptive RED, three‐section RED (TRED), and adaptive queue management with random dropping (AQMRD). The effect of each input factor as well as their interactions are evaluated using factorial design technique that results to obtain the nonlinear equation for performance measure in terms of input factors buffer size, maximum threshold, and the number of FTP sources. Finally, we show the contour plots for variation of performance measure throughput (steady state) from minimum to maximum values with respect to the different setting of input parameters. 相似文献
65.
The investigation of a single pit/defect evolution due to localized corrosion that is commonly observed in a wide range of aluminum alloys is reported. Electrochemical experiments, microscopy and computations were used to predict stresses around a single pit/defect. It was observed that the pit/defect profile changing its shape from slightly conical to more hemispherical shape with increasing corrosion time. Also, stress distribution and levels vary non-linearly around a single pit/defect. The results also indicate that the evolution of these pits/defects may lead to nucleation of a crack over time, which can be predicted from these stresses. 相似文献
66.
The Density of States and the Transport Effective Mass in a Highly Oriented Semiconducting Polymer: Electronic Delocalization in 1D 下载免费PDF全文
67.
Identification of lead compounds with higher molecular weight and lower aqueous solubility has become increasingly prevalent with the advent of high throughput screening. Poor aqueous solubility of these lipophilic compounds can drastically affect the dissolution rate and subsequently the drug absorbed in the systemic circulation, imposing a significant burden of time and money during drug development process. Various pre-formulation and formulation strategies have been applied in the past that can improve the aqueous solubility of lipophilic compounds by manipulating either the crystal lattice properties or the activity coefficient of a solute in solution or both, if possible. However, despite various strategies available in the armor of formulation scientist, solubility issue still remains an overriding problem in the drug development process. It is perhaps due to the insufficient conceptual understanding of solubility and dissolution phenomenon that hinders the judgment in selecting suitable strategy for improving aqueous solubility and/or dissolution rate. This article, therefore, focuses on (i) revisiting the theoretical and mathematical concepts associated with solubility and dissolution, (ii) their application in making rationale decision for selecting suitable pre-formulation and formulation strategies and (iii) the relevant research performed in this field in past decade. 相似文献
68.
Parinita Agarwal Mitali Dabi Mamali Das Khantika Patel Pradeep K. Agarwal 《The International journal of environmental studies》2013,70(4):624-630
Jatropha curcas is an important non feed crop, increasingly important as a biofuel crop. It is hardy and resistant to different stress conditions in the field. In the wastelands of Gujarat (India), it is being grown for land reclamation and for socio-economic benefits. The long coastline in this state also promotes the growth of a large number of halophytes. Exploiting the genetic resource of Jatropha and halophytes for drought and salt-induced gene is an important area of research. For the isolation of genes and to study the molecular mechanism a good qualitative and quantitative RNA is a prerequisite. Jatropha leaves have latex, and therefore isolating RNA using guanidine thiocyanate or cetyltrimethylammonium bromide did not yield desirable quality of RNA. This paper reports a very simple and economical protocol for the isolation of good quality RNA from Jatropha and a few halophytes. The sodium dodecyl sulphate was used as a detergent for lysis of plant cells in the extraction buffer along with bentonite, which inhibits the ribonuclease’s activity. The addition of water saturated phenol in mortar-pestle, during grinding, facilitated better homogenisation of the tissues. Absolute RNA precipitation was obtained with the help of 2-butoxyethanol. Further this RNA was used successfully in preparation of complementary DNA and subsequently used for gene isolation. 相似文献
69.
Popat Y. Orlandi M. Gupta S. Bazzanella N. Pillai S. Patel M. K. Miotello A. Patel N. 《Catalysis Letters》2022,152(2):438-451
Catalysis Letters - Mixed transition-metals oxide electrocatalysts have shown huge potential for electrochemical water oxidation due to their earth abundance, low cost and excellent... 相似文献
70.
Devi Neena Patel Sanjay K. S. Kumar Pradeep Singh Archana Thakur Nandita Lata Jeevan Pandey Deepak Thakur Vikram Chand Duni 《Catalysis Letters》2022,152(4):944-953
Catalysis Letters - In this study, Rhodococcus pyridinivorans cells containing hyperactive acyltransferase was immobilized on various macromolecules based-polymeric matrices and used to improve... 相似文献